Incidence and enterotoxigenic profile of Bacillus cereus in meat and meat products of Uttarakhand, India

Abstract

The present investigation was undertaken to study the incidence and enterotoxigenicity of Bacillus cereus in raw meat and meat products. B. cereus was isolated from 29 (30.9 %) of the 94 samples analyzed. Recorded incidences of B. cereus from raw meat and meat products samples were 27.8 and 35 %, respectively. A high level of organism was found in cooked-meat (35 %) than raw meat samples (27.78 %) from 40 cooked-meat products and 54 raw meat samples analyzed. Screening of isolates by multiplex polymerase chain reaction revealed the overall distribution of various enterotoxin genes hblDAC complex, nheABC complex, cytK and entFM as 55.2, 89.7, 41.4 and 93 %, respectively. The level of contamination with B. cereus was moderately higher in some samples but did not exceed the level which is sufficient to induce food poisoning. A relatively higher incidence of B. cereus in meat products, with the majority of isolates harboring all the enterotoxin genes can pose a potential public health threat.

Prevalence and Characterization of Bacillus cereus in Meat and Meat Products in and around Jammu Region of Jammu and Kashmir

“…In the present study results revealed the incidence of B. cereus isolates as 29.33 per cent in meat and meat products which are in agreement with similar incidence reported by Schlegelova et al, (2003) and Tewari et al, (2013), who too reported an incidence of 28 per cent and 30.9 per cent respectively in meat samples. However, Bedi et al, (2004) and Kamat et al, (1989) reported an incidence of 63.2 per cent and 80 per cent respectively in chicken and meat products.…”

“…However, Willayat et al, (2007), who examined 200 raw mutton and cooked mutton samples found that 47 samples were positive for isolation and further results revealed that contamination of raw meat with Rather et al, (2011), who confirmed 59 (98.3 %) isolates out of 60 by targeting gyrB gene by using PCR. Similarly Tewari et al, (2013) confirmed 29 (100 %) isolates by targeting species specific gyrB gene which supports the results of the present study.…”

Molecular Characterization and Toxin Profiles of Bacillus spp. Isolated from Retail Fish and Ground Beef

“…The prevalence of the hblACD and nheABC complexes in our B. cereus isolates was lower than that obtained by Tewari et al. (). In this study, the detection rates of the hblA , hblC , and hblD genes in B. cereus were 25%, 37.5%, and 37.5%, respectively.…”

“…Among the Bacillus species, B. cereus is a main cause of foodborne emetic and diarrheal diseases due to its enterotoxins and emetic toxins. Previous studies have indicated that B. cereus from various foods commonly has the nheABC , hblACD , and cytK genes (Guinebretiere, Broussolle, & Nguyen‐The, ; Kim et al., ; Tewari et al., ; Yim et al., ). In this study, 25% of the B. cereus isolates had all three genes of HBL and NHE complexes.…”

Bacillus cereus food poisoning: international and Indian perspective

“…In another study, Tewari et al (2013) reported 30.9 % overall incidence of B. cereus in various meat and meat products, while the recorded incidences of B. cereus from raw meat and meat products samples were 27.8 and 35 %, respectively.…”