SUMMARY:The aim of this study is to evaluate the prevalence of agenesis of palmaris longus muscle tendon among school children in Ogun state, Southwestern Nigeria and also, to verify the symmetry of the agenesis of the muscle in relation to sex. The presence of the palmaris longus muscle tendon was determined in 564 individuals, 285 male and 279 female, with age varying from 2 to 20 years old. Four different tests were used; Schaeffer's test, Thompson's test, Mishra's test 1 and Pushpakumar's "two-finger sign" method. The data obtained were further analyzed statistically and the agenesis of palmaris muscle was found to be 26.7% (unilateral 13.0%; bilateral 13.7%). Prevalence was higher in female (32.3%) than male (21.4%) (P>0.05). Besides, we observed higher agenesis in the left side in female individuals. High prevalence of agenesis of palmaris muscle observed in the study population is similar to other previous reports in Southwestern Nigeria.