Incidence of aneuploid spermatozoa among infertile men studied by multicolor fluorescence in situ hybridization

Lähdetie, Jaana; Saari, Nina; Ajosenpaa-Saari, Mari; Mykkänen, Juha

doi:10.1002/(sici)1096-8628(19970711)71:1<115::aid-ajmg21>3.0.co;2-o

Cited by 66 publications

(9 citation statements)

References 34 publications

(45 reference statements)

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“…Applying dual and triple colour FISH, several studies have reported a higher frequency of chromosomal aneuploidies in spermatozoa of infertile men compared with normal fertile donors, at least for some chromosomes (Moosani et al, 1995;Pang et al, 1995Pang et al, , 1999Bernardini et al, 1997;Colombero et al, 1997;Lähdethie et al, 1997;Martin et al, 1997;Rives et al, 1998;Storeng et al, 1998;Vegetti et al, 2000). In addition, the issue of whether older men are at an increased risk of producing aneuploid offspring remains unclear, although some studies have shown a slight effect of age on chromosome numbers in spermatozoa (Griffin et al, 1995;Robbins et al, 1995;Kinakin et al, 1997;Rousseaux et al, 1998;Asada et al, 2000;Bosch et al, 2001;Sloter et al, 2004;Rubes et al, 2005;Wyrobek et al, 2006) while other studies could not detect age-related effects on tested chromosomes in sperm McInnes et al, 1998;Luetjens et al, 2002;Dakouane et al, 2005a,b).…”

Section: Discussionmentioning

confidence: 99%

The effects of age on the incidence of aneuploidy rates in spermatozoa of oligoasthenozoospermic patients and its relationship with ICSI outcome

et al. 2006

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The development of intracytoplasmic sperm injection (ICSI) for treatment of infertility as a result of severe male factor has improved the chances of achieving pregnancy in many infertile couples. However, concerns have been raised regarding the safety of this technique, because natural sperm selection is bypassed. In the present study, 25 oligoasthenozoospermic patients who were divided into two groups according to age: group A, 20-34 (n = 10) and group B, 35-50 (n = 15), were included. Pooling the data of the three semen parameters that were tested (volume, concentration and progressive motility) no statistically significant difference between the two age groups was found. A total of 50 883 decondensed spermatozoa was analysed using the dual and triple colour fluorescence in situ hybridization to estimate the rates of aneuploidy for chromosomes 13, 18, 21, X and Y in the two age groups. There was a significantly higher incidence of disomy for chromosome 21 compared to the other autosomes (chromosomes 13 and 18) in both age groups. The disomy rate of XY was significantly higher in the younger subject group (0.1%) compared to the older group (0.05%, p < 0.05). Statistically significant differences in the mean number of clinical pregnancies and abortions were not observed between the two age groups. The aneuploidy rates for all the analysed chromosomes did not differ significantly, both between and within the two age groups, and as a result there seems to be no effect of male age on chromosome numbers in the spermatozoa and on the ICSI outcome.

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Section: Discussionmentioning

confidence: 99%

The effects of age on the incidence of aneuploidy rates in spermatozoa of oligoasthenozoospermic patients and its relationship with ICSI outcome

et al. 2006

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“…In normal individuals disomies are around 0.1 % for most autosomes and 0.3 % for chromosome 21 and the sex chromosomes. Increases in sperm aneuploidies in individuals showing oligoasthenoteratozoospermia (OTA) have been reported by many authors [8,10,[78][79][80][81][82][83][84][85][86][87][88][89][90][91]. Furthermore, an increase in aneuploid sperm has been detected in individuals with alterations of any of the basical semen parameters: sperm number [89,92], sperm motility [10] and sperm morphology [80,89,[93][94][95][96][97].…”

Section: Infertile Individuals With a Normal Karyotypementioning

confidence: 99%

FISH studies of chromosome abnormalities in germ cells and its relevance in reproductive counseling

et al. 2005

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Chromosome abnormalities are one of the major causes of human infertility. In infertile males, abnormal karyotypes are more frequent than in the general population. Furthermore, meiotic disorders affecting the germ cell-line have been observed in men with normal somatic karyotypes consulting for infertility. In both cases, the production of unbalanced spermatozoa has been demonstrated. Basically addressed to establish reproductive risks, fluorescence in situ hybridization (FISH) on decondensed sperm heads has become the most frequently used method to evaluate the chromosomal constitution of spermatozoa in carriers of numerical sex chromosome abnormalities, carriers of structural chromosome reorganizations and infertile males with normal karyotype. The aim of this review is to present updated figures of the information obtained through sperm FISH studies with an emphasis on its clinical significance. Furthermore, the incorporation of novel FISH-based techniques (Multiplex-FISH; Multi-FISH) in male infertility studies is also discussed.

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“…The purpose of this work was to study the potential contribution of fungicides and common lifestyle exposures (smoking and alcohol) on the frequencies of aneuploid sperm in 32 healthy farmers. Age and sperm concentration were analyzed as potential confounders, because several previous studies [Griffin et al, 1995;Lähdetie et al, 1997;Martin and Rademaker, 1987;Martin et al, 1995;Moosani et al, 1995;Robbins et al, 1995] have suggested an association between these factors and sperm aneuploidy.…”

Section: Introductionmentioning

confidence: 99%

Aneuploidy in sperm and exposure to fungicides and lifestyle factors

Härkönen

Viitanen

Larsen

et al. 1999

Environ. Mol. Mutagen.

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Fungicides include chemicals that are known aneugens. The purpose of the present study was to investigate whether occupational exposure to these and other agricultural pesticides induces aneuploidy in human sperm. The contribution of lifestyle factors (smoking and alcohol consumption) to the frequency of aneuploid sperm was evaluated as well. The effects of age and sperm concentration were analyzed as confounders. Spermatozoa from 30 healthy farmers were studied before and after exposure to fungicides, using fluorescence in situ hybridization (FISH). Ten thousand spermatozoa were scored per semen sample to determine the disomy and diploidy frequencies for chromosomes 1 and 7. Exposure to fungicides was not associated with sperm aneuploidy. Smoking was significantly associated with sperm carrying an extra chromosome 1 and with diploid sperm as well as with the aggregate frequency of aneuploid sperm. Alcohol consumption, sperm concentration, and age showed inconsistent results before and after the season of exposure to fungicides. For low‐level exposures, such as occupational exposures, the sensitivity of the sperm‐FISH method may not be sufficient. The present study supports earlier ones showing that smoking can increase aneuploidy in human sperm. Environ. Mol. Mutagen. 34:39–46, 1999 © 1999 Wiley‐Liss, Inc.

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Incidence of aneuploid spermatozoa among infertile men studied by multicolor fluorescence in situ hybridization

Cited by 66 publications

References 34 publications

The effects of age on the incidence of aneuploidy rates in spermatozoa of oligoasthenozoospermic patients and its relationship with ICSI outcome

The effects of age on the incidence of aneuploidy rates in spermatozoa of oligoasthenozoospermic patients and its relationship with ICSI outcome

FISH studies of chromosome abnormalities in germ cells and its relevance in reproductive counseling

Aneuploidy in sperm and exposure to fungicides and lifestyle factors

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