Incidence of apoptosis after retinoids and insulin-like growth factor-I (IGF-I) supplementation during goat in vitro embryo production

Abstract

The addition of growth factors and vitamins enhances goat embryonic development in vitro. However, few attempts have been reported trying to identify supplementation regimens for oocyte maturation or embryo culture with additive properties. The present report was aimed to evaluate if retinoids [0.3 μM retinyl acetate (RAc) and 0.5 μM 9-cis-retinoic acid (RA)] supplementation during goat oocyte maturation and retinoids and/or 50 ng mL-1 IGF-I during embryo culture synergically enhanced embryonic development while diminishing the incidence of apoptosis. All combinations of RAc and RA treatment produced blastocysts with similar efficiencies, while IGF-I enhanced embryos yields irrespectively of retinoid addition. Moreover, retinoids and IGF-I supplementation showed similar caspase activity or DNA fragmentation indexes in blastocysts. In conclusion, supplementation with retinoids and IGF-I during goat embryo culture enhances blastocysts development without synergic reduction of apoptosis.

Antioxidant and developmental capacity of retinol on thein vitroculture of rabbit embryos

“…The in vitro production of embryos has become a traditional tool to increase the reproductive efficiency, to augment the genetic gain and to reduce generation intervals in livestock animals (Keefer et al , 2001; Peippo et al , 2001; Conceição et al , 2016). However, retardation and low development rate of embryos have been induced due to their exposure to free radicals and reactive oxygen species (ROS) that arise during in vitro culture conditions (Agarwal et al , 2003; Pasqualotto et al , 2004; Mehaisen & Saeed, 2015).…”

Retinoic acid improves maturation rate and upregulates the expression of antioxidant-related genes inin vitromatured buffalo (Bubalus bubalis) oocytes

“…In contrast, basic maturation media supplemented with FSH and 500 nM RA improved blastocyst formation of bovine embryos cultured in potassium simplex optimized medium (KSOM) [32]. In another study, the same concentration of RA improved the developmental capacity in goat embryos [33] or even with higher concentration (2 μM), it showed beneficial effects on mouse oocyte IVM [34]. One explanation of these contrary results may be the different used media with different compositions in which interaction between RA and hormones supplemented to the maturation media has been proved to affect the action mechanisms inside cells and oocytes [30].…”

Effect of cysteine, insulin-like growth factor-1 and epidermis growth factor during in vitro oocyte maturation and in vitro culture of yak-cattle crossbred embryos

“…EGF and IGF-1 are involved in regulating cell proliferation and apoptosis (Paria and Dey 1990;Wasielak and Bogacki 2007;Chen et al 2017). Positive effects on oocyte maturation and/or embryonic development have been observed if a single or different combination of Cys, EGF and IGF-1 concentrations in IVM, IVF and IVC media is optimal in cattle (Ali et al 2003;Sirisathien et al 2003;Neira et al 2010;Nabenishi et al 2012), pig (Choe et al 2010;Lott et al 2011), sheep (Shabankareh and Zandi 2010), buffalo (Pawshe et al 1998), human (Yu et al 2012), mouse (Toori et al 2014); cat (Thongkittidilok et al 2015), goat (Conceicao et al 2016;Zhou et al 2016), canine (Sato et al 2018) and yak (Pan et al 2015;Chen et al 2017).…”