Incidence of Bovine Clinical Mastitis Caused by Escherichia coli

Manasa, None V.; Kumar, T. Venkata Sai; Rao, T. Prasada; Kumar, Kamal; Sireesha, K.

doi:10.20546/ijcmas.2019.805.142

Cited by 6 publications

(11 citation statements)

References 15 publications

(17 reference statements)

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“…Very low percent of isolation was reported by Schukken et al (2009) they isolated higher percentage of E. coli (16.2%) followed by the S. aureus (9.6 %) and Str. agalactiae (0.17%) which showed higher prevalence of gram negative organisms and same was reported recently Manasa et al (2019).…”

Section: Isolation and Identification Of Bacteria From Clinical Mastitis Milksupporting

confidence: 87%

Isolation, Molecular Identification and Multidrug Resistance Profiling of Bacteria Causing Clinical Mastitis in Cows

Vatalia

Bhanderi

Nimavat

et al. 2020

ASD

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Background: Mastitis, the inflammation of parenchyma of mammary gland is frequently considered to be costliest and complex disease prevalent in India. Mastitis is caused by pathogens like Staphylococcus spp., Streptococcus spp., Mycoplasma bovis, E. coli, Klebsiella spp., Citrobacter spp., Enterobacter spp. and Entercoccus. The treatment of mastitis in animals is carried out using antibiotics. Treatment failure in mastitis is due to increased antibiotic resistance of mastitis pathogens and also due to indiscriminate use of antibiotics without testing in vitro antibiotic sensitivity test against causal organisms. In comparison to cultural method, PCR assays takes less time for detection of bacteria from the mastitis milk samples. Present research work was carried out regarding isolation, identification and multiple drug resistance profile of clinical bovine mastitis associated pathogens using conventional as well as molecular approach. Methods: In the present study, 73 mastitis milk samples were collected from Anand and Panchmahal district of Gujarat. The milk samples were subjected for cultural isolation and DNA extraction for identification of bacteria by cultural and PCR method. Antimicrobial sensitivity pattern of the isolates were carried by disc diffusion method and isolates were categorized in multiple drug resistant. Result: In the present study, Out of 73 mastitis milk samples collected from cows 48 (65.75%) cows were positive for bacterial isolation and S. aureus was the most predominant bacterial species. PCR from the mastitis milk additionally detected bacteria in culturally negative milk samples. Most sensitive drug was gentamicin and most of the isolates (90.19%) showed the multiple drug resistance for the two to nine drugs with 0.1 to 0.6 multiple antibiotic resistance index.

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Section: Isolation and Identification Of Bacteria From Clinical Mastitis Milksupporting

confidence: 87%

Isolation, Molecular Identification and Multidrug Resistance Profiling of Bacteria Causing Clinical Mastitis in Cows

Vatalia

Bhanderi

Nimavat

et al. 2020

ASD

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“…In addition, Barley straw exhibits a high count of TBC, and flax straw displays high values for both Klebsiella spp. and coliforms Microorganisms that cause mastitis can be related to the environmental transmission of pathogens (Manasa et al, 2019). E coli and Klebsiella spp.…”

Section: Resultsmentioning

confidence: 99%

Properties of conventional and alternative bedding materials for dairy cattle

Ferraz

Leso

et al. 2020

Journal of Dairy Science

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The bedding material used in barns for dairy cows has a significant effect on animal welfare and performance. Bedding influences the duration in which animals remain lying down and, consequently, the processes of rumination and milk production. It is crucial to have a complete understanding of the properties of bedding materials and the effects of alternative bedding materials on dairy cattle. This paper aims to evaluate the physical, chemical, and biological properties of various alternative and conventional bedding materials for dairy cattle for use in compost bedded pack or freestall barn systems. We analyzed 50 samples of 17 bedding materials produced in 3 European countries. We analyzed physical properties including the water holding capacity, porosity, moisture content, bulk density, dry bulk density, and particle size. Chemical analyses were performed to determine the total N, total organic C, and C:N ratio. In the biological analyses, the Escherichia coli count, total bacteria count, coliform count, and Klebsiella spp. count were assessed. The results demonstrated how the physical properties of the bedding materials may influence the chemical and biological properties. All of the materials presented adequate chemical properties to be used as bedding material. The physical properties of the bedding materials differed widely among the materials except for the dry bulk density, which presented no difference. Moreover, the contamination of each studied microorganism was observed for each bedding material to determine which material had the lowest level of contamination. Posidonia oceanica, Miscanthus grass, and spelt husks could be considered as a potential alternative material for use as bedding material for dairy cows in both systems (i.e., composted bedded pack and freestall). This ex-periment illustrated the importance of performing thorough physical, chemical, and biological analyses before implementing a material as bedding for dairy cattle.

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“…Ninety two milk samples were collected from the udder lobe from dairy cows with subclinical mastitis, which were positive as a result of the California Mastitis Test. These samples were delivered to the laboratory under cold chain conditions (8,42,58). The samples not to be included in the analysis immediately were frozen at (-20) °C and stored.…”

Section: Materials and Samplingmentioning

confidence: 99%

“…and E. coli isolates were performed based on disc diffusion method according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) standards (6,12,18,19,26). The antibiotic discs used in this study were selected not only for which they are used in the treatment of ruminants (21,29,38,42) but also observe for ESBL production and carbapenem resistance. For this purpose, a member of penicillins, cephalosporins, carbapenems quinolones, sulphonamides, aminoglycosides, macrolides, tetracyclines were used in disc diffusion tests.…”

Section: Determination Of Antibiotic Susceptibilities Of Enterobacteriaceae Isolatesmentioning

confidence: 99%

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First detection of carbapenem resistance in Enterobacteriaceae isolates isolated from dairy cows’ mastitis infection in Türkiye

Babacan

2022

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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With this study, carbapenem resistance genes were declared for the first time in Enterobacteriaceae isolates isolated from dairy cows' mastitis infection in Turkey. In the bacteriological examination of 212 milk samples, 14 (6.60%) E. coli, three (1.41%) Klebsiella oxytoca, and two (0.94%) Klebsiella pneumonia were isolated.At least two E. coli isolates were found to be resistant to all of the antibiotics used in the antibiogram test. The highest resistance was found against cefotaxime and amoxicillin in K. oxytoca isolates. According to the results of PCR targeting blaCTX-M, blaTEM, and blaSHV genes, the blaCTX-M gene was detected in one K. oxytoca and four E. coli isolates, which were found ESBL positive. According to the results of PCR targeting carbapenem and colistin resistance genes, the IMP gene was detected in four E.coli, one K. oxytoca, and one K. pneumonia isolates. OXA-48-like gene was detected in two E. coli isolates. This two E. coli isolates were also IMP gene positive. While NDM gene was detected in two E. coli, KPC gene was detected in one E. coli isolate. One of the colistin resistance genes, mcr-1 was detected in two E.coli strains with PCR. This study showed that ESBL production and carbapenem resistance in Enterobacteriaceae family strains to become prevalent and increasing, especially among E. coli isolates.Furthermore, identification of multiple antibiotic resistance in the isolates indicated that antibiotic resistance also spread rapidly and increased.

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Incidence of Bovine Clinical Mastitis Caused by Escherichia coli

Cited by 6 publications

References 15 publications

Isolation, Molecular Identification and Multidrug Resistance Profiling of Bacteria Causing Clinical Mastitis in Cows

Isolation, Molecular Identification and Multidrug Resistance Profiling of Bacteria Causing Clinical Mastitis in Cows

Properties of conventional and alternative bedding materials for dairy cattle

First detection of carbapenem resistance in Enterobacteriaceae isolates isolated from dairy cows’ mastitis infection in Türkiye

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