A field experiment entitled “Effect of Foliar Application of Nano-Urea under Different Nitrogen Levels on Productivity and Quality of Pearl millet (Pennisetum glaucum L.)” was conducted at Research Farm, Vivekananda Global University, Jaipur (India) during Kharif season of 2021. The experiment was laid out with 9 treatment combinations comprising in a factorial randomized block design with three replications. Results showed that highest plant height, dry matter accumulation, chlorophyll content, nitrogen content, phosphorus content and potassium content in grain and straw of pearl millet was obtained with the application of 100% RDN which was significantly superior to 50% RDN. Results further showed that foliar spray of Nano-Urea (4 ml/l water) at 30 and 45 DAS significantly increased the plant height, dry matter accumulation, chlorophyll content, nitrogen content, phosphorus content and potassium content in grain and straw of pearl millet over control and foliar spray of Nano-Urea (4 ml/l water) at 30 DAS.