Incidence of hemagglutination-inhibiting antibodies for bhanja virus in humans along the north-west border of yugoslavia

Punda, V.; Ropac, Darko; Vesenjak-Hirjan, J

doi:10.1016/s0176-6724(87)80170-0

Cited by 6 publications

(6 citation statements)

References 3 publications

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“…More studies indicated the presence of BHAV in central Europe [15,17,24] around the Mediterranean basin, across the middle-eastern countries to India and in subsaharan Africa [4,8,22]. However, the strains north of the Sahara are serological distinct from the subsaharan strains [14].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Bhanja virus and Palma virus, two tick-borne phleboviruses

et al. 2012

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Section: Introductionmentioning

confidence: 99%

Genetic characterization of Bhanja virus and Palma virus, two tick-borne phleboviruses

et al. 2012

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“…BHAV was isolated in India in 1954 from a tick on a paralyzed goat and causes fever and signs of central nervous system involvement in young ruminants but not in adult animals. A few cases of febrile illness in humans have been described, and serological surveys in Eastern Europe suggest that BHAV is endemic in that region and may cause undetected human infection (6,25). Two recent papers report nucleotide sequence determination of Bhanja group viruses (26,27) and show that they are related to SFTSV and HRTV.…”

mentioning

confidence: 99%

Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus

Brennan

Zhang

et al. 2015

J Virol

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Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne pathogen that was first reported in China in 2009. Phylogenetic analysis of the viral genome showed that SFTS virus represents a new lineage within the Phlebovirus genus, distinct from the existing sandfly fever and Uukuniemi virus groups, in the family Bunyaviridae. SFTS disease is characterized by gastrointestinal symptoms, chills, joint pain, myalgia, thrombocytopenia, leukocytopenia, and some hemorrhagic manifestations with a case fatality rate of about 2 to 15%. Here we report the development of reverse genetics systems to study STFSV replication and pathogenesis. We developed and optimized functional T7 polymerase-based M-and S-segment minigenome assays, which revealed errors in the published terminal sequences of the S segment of the Hubei 29 strain of SFTSV. We then generated recombinant viruses from cloned cDNAs prepared to the antigenomic RNAs both of the minimally passaged virus (HB29) and of a cell culture-adapted strain designated HB29pp. The growth properties, pattern of viral protein synthesis, and subcellular localization of viral N and NSs proteins of wild-type HB29pp (wtHB29pp) and recombinant HB29pp viruses were indistinguishable. We also show that the viruses fail to shut off host cell polypeptide production. The robust reverse genetics system described will be a valuable tool for the design of therapeutics and the development of killed and attenuated vaccines against this important emerging pathogen. IMPORTANCESFTSV and related tick-borne phleboviruses such as Heartland virus are emerging viruses shown to cause severe disease in humans in the Far East and the United States, respectively. Study of these novel pathogens would be facilitated by technology to manipulate these viruses in a laboratory setting using reverse genetics. Here, we report the generation of infectious SFTSV from cDNA clones and demonstrate that the behavior of recombinant viruses is similar to that of the wild type. This advance will allow for further dissection of the roles of each of the viral proteins in the context of virus infection, as well as help in the development of antiviral drugs and protective vaccines.T he family Bunyaviridae is one of the largest taxonomic groupings of RNA viruses, containing over 350 named isolates that are classified into five genera: Hantavirus, Nairovirus, Orthobunyavirus, Phlebovirus, and Tospovirus (1). All viruses share a genome structure that comprises three segments of negative-sense or ambisense RNA, named small (S), medium (M), and large (L). The S segment encodes the nucleocapsid (N) protein; the M segment encodes the virion glycoproteins Gn and Gc; and the L segment encodes the L protein, the viral RNA-dependent RNA polymerase. Some viruses also encode nonstructural proteins on the S and M segments. Viruses that impinge on human health, either directly by causing human disease or indirectly by causing economic losses of domestic animals or crop plants, are found in each of the five gener...

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“…The adults have a bimodal activity with peaks from March to June and September to November [ 9 ]. Although, it was observed that an important impact on the interpretation of seasonal dynamics was the methodology used for tick collecting [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of Bhanja Bandavirus in Patients with Neuroinvasive Disease of Unknown Etiology in Croatia

Vilibic-Cavlek,

Stevanovic,

Krcmar

et al. 2023

Microorganisms

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Background: Although the Bhanja bandavirus (BHAV) is widely distributed in some European countries, human infections are rarely reported. This study analyzed the prevalence of BHAV antibodies in patients with neuroinvasive diseases of unsolved etiology. Methods: A total of 254 Croatian patients who developed neurological symptoms during the four consecutive arbovirus transmission seasons (April 2017–October 2021) were tested. Cerebrospinal fluid (CSF) and urine samples were tested using RT-qPCR. In addition, CSF and serum samples were tested using a virus neutralization test. Results: BHAV RNA was not detected in any samples, while neutralizing (NT) antibodies were detected in serum samples of 53/20.8% of patients (95% CI = 16.0–26.3). In two patients, BHAV NT antibodies were detected in the CSF, indicating a recent infection. Both patients were inhabitants of rural areas in continental Croatia, and one reported a tick bite two weeks before symptoms onset. The seropositivity was high in all age groups (15.2–29.1%). The majority of seropositive patients (94.3%) resided at altitudes less than 200 m above sea level. The prevalence rates correlated positively with population density and negatively with certain climate parameters (temperature, number of hot/warm days). Conclusions: The presented results indicate that BHAV is distributed in Croatia. Further studies are needed to determine the clinical significance of this neglected arbovirus.

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Incidence of hemagglutination-inhibiting antibodies for bhanja virus in humans along the north-west border of yugoslavia

Cited by 6 publications

References 3 publications

Genetic characterization of Bhanja virus and Palma virus, two tick-borne phleboviruses

Genetic characterization of Bhanja virus and Palma virus, two tick-borne phleboviruses

Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus

Detection of Bhanja Bandavirus in Patients with Neuroinvasive Disease of Unknown Etiology in Croatia

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