Incidence of high sperm DNA fragmentation in a targeted population of subfertile men

Vinnakota, Chitra; Cree, Lynsey M.; Peek, John C.; Morbeck, Dean E.

doi:10.1080/19396368.2019.1668077

Cited by 19 publications

(15 citation statements)

References 54 publications

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“…Studies have investigated the correlation between sperm DFI and conventional semen parameters. 34 35 36 These studies found that infertile men tend to have more sperm DNA damage than fertile men, and sperm DFI was negatively associated with sperm concentration, vitality, and normal morphology. In addition, sperm DFI was positively correlated with age, abstinence time, and unhealthy lifestyles.…”

Section: Discussionmentioning

confidence: 94%

Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer

Wang

et al. 2022

Asian Journal of Andrology

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Damage to sperm DNA was proposed to play an important role in embryonic development. Previous studies focused on outcomes after fresh embryo transfer, whereas this study investigated the influence of sperm DNA fragmentation index (DFI) on laboratory and clinical outcomes after frozen embryo transfer (FET). This retrospective study examined 381 couples using cleavage-stage FET. Sperm used for intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) underwent density gradient centrifugation and swim up processing. Sperm DFI had a negative correlation with sperm motility ( r = −0.640, P < 0.01), sperm concentration ( r = −0.289, P < 0.01), and fertilization rate of IVF cycles ( r = −0.247, P < 0.01). Sperm DFI examined before and after density gradient centrifugation/swim up processing was markedly decreased after processing (17.1% vs 2.4%, P < 0.01; 65 randomly picked couples). Sperm progressive motility was significantly reduced in high DFI group compared with low DFI group for both IVF and ICSI (IVF: 46.9% ± 12.4% vs 38.5% ± 12.6%, respectively; ICSI: 37.6% ± 14.1% vs 22.3% ± 17.8%, respectively; both P < 0.01). The fertilization rate was significantly lower in high (≥25%) DFI group compared with low (<25%) DFI group using IVF (73.3% ± 23.9% vs 53.2% ± 33.6%, respectively; P < 0.01) but was equivalent in high and low DFI groups using ICSI. Embryonic development and clinical outcomes after FET were equivalent for low and high DFI groups using ICSI or IVF. In this study, sperm DFI did not provide sufficient information regarding embryo development or clinical outcomes for infertile couples using FET.

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Section: Discussionmentioning

confidence: 94%

Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer

Wang

et al. 2022

Asian Journal of Andrology

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“…A significant, although relatively low, correlation was found in the percentage of normal sperm heads, which is likely to be more age related than the condition of other sperm parts. Vinnakota et al 33 examined a large group of subfertile patients and sperm donors and found that men with a high DFI were older and exhibited a lower percentage of motile sperm than men with a normal DFI. On the strength of their results, they recommended the testing of chromatin integrity in patients over age 45 with sperm motility <40%.…”

Section: Discussionmentioning

confidence: 99%

The effects of age onDNAfragmentation, the condensation of chromatin and conventional semen parameters in healthy nonsmoking men exposed to traffic air pollution

Rubeš

Sipek

Kopecka

et al. 2021

Health Science Reports

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Background Numerous studies have investigated age‐based declines in semen traits, but the impact of paternal age on semen parameter values remains inconclusive. Objectives The aim of this study was to detect an impact of age on semen quality was studied in healthy nonsmoking men exposed to traffic air pollution. Methods Semen samples from 150 Prague City policemen aged 23 to 63 years were examined for standard semen parameters, sperm DNA fragmentation and high DNA stainability. Results A significant positive correlation was found between age and %DFI ( r = .359, P < .001), and negative correlations were found between age and sperm vitality ( r = −.247, P < .001), the % acrosome‐intact sperm ( r = −.202, P = .013) and the % normal sperm heads ( r = −.204, P = .012). A weak but significant negative correlation was found for high DNA stainability (% HDS) vs age ( r = −.161, P = .050). No significant correlation was detected between male age and the other investigated semen quality parameters. At ages of 23 to 30, 31 to 40, 41 to 50, and 51 to 63 years, the mean %DFI values were 12.7 ± 7.18, 14.7 ± 7.42, 19.6 ± 11.25, and 34.2 ± 15.08, respectively. Conclusion Our study shows a strong relationship ( P < .001) between the age of men and sperm DNA fragmentation in an occupational cohort at risk of exposure to heavy traffic‐related air pollution in a large city center.

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“…However, DFI is not only affected by age, but also by indulgences such as cigarettes (18) and alcohol (19), lifestyle habits such as sleep and exercise, and intake of supplements (20). Thus, various aspects of aging, multiple stress factors, and negative factors affecting the DFI require further investigations (21). In terms of the link with IVF outcomes, it has been reported that, although there is no difference in the fertilization rate and embryonic development rate between high DFI and low DFI (22), a high DFI tends to be associated with higher rates of miscarriage, resulting in a low live birth rate per transplantation (23,24).…”

Section: Discussionmentioning

confidence: 99%

Correlation Between Semen Examination Based on the WHO Criteria and Sperm DNA Fragmentation Test

Okubo

Onda

Hayashi

et al. 2021

Preprint

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Purpose: We performed and analyzed the DFI test and semen test based on the WHO criteria and compared the two using semen factors. Methods: DFI tests and general semen tests were conducted in accordance with the WHO criteria, and correlations between the two tests were investigated. The WHO criteria were set as the cutoff value for each of the following factors: fluid volume, concentration, motility, and normal morphology, and compared with the DFI results.Results: The subjects had a mean DFI of 15.6±11.5% and the DFI increased with age. In contrast, the concentration, motility, and normal morphology decreased as the DFI increased. Patients who satisfied the WHO criteria in terms of sperm concentration, motility, and normal morphology had a significantly lower DFI than the group that did not satisfy the criteria. Therefore, evaluation with a general semen test based on the WHO criteria should be regarded as a qualitative evaluation of all factors other than fluid volume.Conclusions: Male infertility due to sperm DNA fragmentation should be suspected when in vitro fertilization (IVF) results are poor despite normal semen findings based on the WHO criteria. The results of this study suggest that the DFI test may be an important evaluation in follow-up semen analyses.

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Incidence of high sperm DNA fragmentation in a targeted population of subfertile men

Cited by 19 publications

References 54 publications

Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer

Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer

The effects of age onDNAfragmentation, the condensation of chromatin and conventional semen parameters in healthy nonsmoking men exposed to traffic air pollution

Correlation Between Semen Examination Based on the WHO Criteria and Sperm DNA Fragmentation Test

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