Incidence of human parvovirus B19 DNA detection in blood donors

Yoto, Yuko; Kudoh, Tooru; Haseyama, Keiji; Suzuki, Nobuhiro; Oda, Takanori; Katoh, Takanori; Takahashi, Tsuneo; Sekiguchi, S.; Chiba, Shunzo

doi:10.1111/j.1365-2141.1995.tb05427.x

Cited by 80 publications

(59 citation statements)

References 11 publications

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“…Testing minipools of 10 instead of 50 to 1,200 plasma samples also affected detection. For pools of 10 plasma samples, Yoto et al found a 0.6% prevalence of erythrovirus DNA with a nested PCR assay that had a sensitivity of 100 genome equivalents (geq)/ml (42). The recent identification of at least three fairly distant human erythrovirus genotypes might also have played a role in estimations of the prevalence of circulating viral DNA (35).…”

Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Persistent Human Erythrovirus Infection in Blood Donor Samples

Candotti

Etiz²,

Parsyan

et al. 2004

J Virol

168

182

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The presence of human erythrovirus DNA in 2,440 blood donations from the United Kingdom and subSaharan Africa (Ghana, Malawi, and South Africa) was screened. Sensitive qualitative and real-time quantitative PCR assays revealed a higher prevalence of persistent infection with the simultaneous presence of immunoglobulin G (IgG) and viral DNA (0.55 to 1.3%) than previously reported. This condition was characterized by a low viral load (median, 558 IU/ml; range, 42 to 135,000 IU/ml), antibody-complexed virus, free specific IgG, and potentially infectious free virus. Human erythrovirus genotype 1 (formerly parvovirus B19) was prevalent in the United Kingdom, Malawi, and South Africa. In contrast, only human erythrovirus genotype 3 (erythrovirus variant V9) was prevalent in Ghana. Genotype 3 had considerable genetic diversity, clustering in two probable subtypes. Genotype 1-based antibody assays failed to detect 38.5% of Ghanaian samples containing antibodies to genotype 3 virus but did not fail to detect cases of persistent infection. This study indicates a potential African origin of genotype 3 human erythrovirus and considerable shortcomings in the tools currently used to diagnose erythrovirus infection. Human erythrovirus (formerly parvovirus B19) is a member of the genus Erythrovirus within the family Parvoviridae (24).Erythrovirus infection occurs frequently in humans. The prevalence of specific immunoglobulin G (IgG) antibodies is 2 to 15% in young children, 30 to 60% in adults, and more than 85% in those 70 years old or older (14). The linear singlestranded DNA genome of this small, nonenveloped virus is about 5 kb long and contains two large open reading frames (ORFs). The first ORF encodes nonstructural protein NS1, and the second one encodes both major VP2 and minor VP1 structural capsid proteins. VP1 consists of a unique sequence of 227 amino acids (VP1u) and is followed by the entire VP2 sequence (554 amino acids). Two additional ORFs encoding small proteins (7.5 and 11 kDa) with unknown functions have also been described (see reference 14 for a review).Following viral infection in immunocompetent individuals, the predominant immune response is a humoral response, which is assumed to confer protective, lifelong immunity. The early IgM response is directed against VP2, while the mature response mostly involves the production of IgG to VP1 (see reference 14 for a review). Several VP2 and VP1u regions containing neutralizing epitopes have been identified (10, 32, 43). However, neutralizing linear epitopes seem to cluster in the N terminus of VP1u and the VP1-VP2 junction regions and to elicit a more efficient response than the epitopes in the VP2 region, which are mainly conformational epitopes (21,26,28,31,36). The inability to develop an efficient neutralizing immune response, as observed mainly in immunosuppressed individuals but also in otherwise healthy individuals, may result in the failure to eliminate the virus, thus leading to persistent infection and the possible occurrence of chronic diseases, suc...

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Section: Discussionmentioning

confidence: 99%

Identification and Characterization of Persistent Human Erythrovirus Infection in Blood Donor Samples

Candotti

Etiz²,

Parsyan

et al. 2004

J Virol

168

182

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“…Esta frecuencia se encuentra entre las más altas reportadas previamente en la literatura para este tipo de poblaciones y es cercana a la descrita por Yoto y cols, quienes notifi caron una frecuencia de 1 en 167 y Candotti y cols, que encontraron un rango 0,55 a 1,3% 11,18 . No obstante, otros autores han documentado presencia de ADN de B19 en un amplio rango de frecuencias, las que fl uctúan entre 1 en 35.000 y 1 en 625 10,[19][20][21][22] .…”

Section: Discussionunclassified

“…El uso de grandes lotes de plasmas, con la consecuente dilución de las muestras, puede resultar en una subestimación de la presencia de ADN viral por pérdida en la detección de aquellos casos con niveles bajos de viremia 11 . Esto concuerda con el hecho de que los estudios que revelan frecuencias de detección más altas, coinciden en la utilización de pequeños lotes de no más de 10 plasmas, con el fi n de evitar el problema de dilución.…”

Section: Discussionunclassified

“…Por otra parte, se ha documentado la presencia de genoma viral en 27 de 16.859 donantes de sangre en Bélgica (1 en 625) 17 . En forma similar, en un estudio realizado en 9.568 unidades de sangre en Estados Unidos de Norteamérica, se demostró la presencia de ADN viral en 11 de ellas (1 en 870) 10 , al igual que en un estudio realizado en Japón, en el cual se detectó la presencia del virus en 6 de 1.000 donantes de sangre (1 en 167) 11 .…”

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Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile

et al. 2011

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“…With techniques like PCR, the frequency of a viremic blood donation ranges from 1:167 (Santagostino et al 1994, Yoto et al 1995 to 1:1420 during seasonal outbreaks (Schmidt et al 2001) and from 1:5950 to 1:30000 off-season , McOmish et al 1993, Schmidt et al 2001.…”

Section: Discussionmentioning

confidence: 99%

Viremic blood donor found by a rapid screening method in a season of high human parvovirus B19 activity in Niterói, Rio de Janeiro, Brazil

Setúbal

Cárdias

Oliveira

et al. 2004

Mem. Inst. Oswaldo Cruz

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Incidence of human parvovirus B19 DNA detection in blood donors

Cited by 80 publications

References 11 publications

Identification and Characterization of Persistent Human Erythrovirus Infection in Blood Donor Samples

Identification and Characterization of Persistent Human Erythrovirus Infection in Blood Donor Samples

Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile

Viremic blood donor found by a rapid screening method in a season of high human parvovirus B19 activity in Niterói, Rio de Janeiro, Brazil

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