Incidence of
            <i>Klebsiella</i>
            Species in Surface Waters and Their Expression of Virulence Factors

Podschun, Rainer; Pietsch, S.; Höller, Christiane; Ullmann, U.

doi:10.1128/aem.67.7.3325-3327.2001

Cited by 168 publications

(134 citation statements)

References 23 publications

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“…The environment can contain a large variety of K. pneumoniae strains with pathogenic potential (26,30), and mastitis cases within a dairy herd are usually caused by many different strains (3,(23)(24)(25). Predominance of a single K. pneumoniae strain, such as RAPD type A in the first outbreak of CM described here, is unusual.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of TwoKlebsiella pneumoniaeMastitis Outbreaks on a Dairy Farm in New York State

et al. 2007

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Klebsiella spp. have become an important cause of clinical mastitis in dairy cows in New York State. We describe the occurrence of two Klebsiella mastitis outbreaks on a single dairy farm. Klebsiella isolates from milk, feces, and environmental sources were compared using random amplified polymorphic DNA (RAPD)-PCR typing. The first mastitis outbreak was caused by a single strain of Klebsiella pneumoniae, RAPD type A, which was detected in milk from eight cows. RAPD type A was also isolated from the rubber liners of milking machine units after milking of infected cows and from bedding in the outbreak pen. Predominance of a single strain could indicate contagious transmission of the organism or exposure of multiple cows to an environmental point source. No new cases with RAPD type A were observed after implementation of intervention measures that targeted the prevention of transmission via the milking machine as well as improvement of environmental hygiene. A second outbreak of Klebsiella mastitis that occurred several weeks later was caused by multiple RAPD types, which rules out contagious transmission and indicates opportunistic infections originating from the environment. The diversity of Klebsiella strains as quantified with Simpson's index of discrimination was significantly higher for isolates from fecal, feed, and water samples than for isolates from milk samples. Several isolates from bedding material that had the phenotypic appearance of Klebsiella spp. were identified as being Raoultella planticola and Raoultella terrigena based on rpoB sequencing.

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Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology of TwoKlebsiella pneumoniaeMastitis Outbreaks on a Dairy Farm in New York State

et al. 2007

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“…The accurate identification of K. pneumoniae strains is increasingly necessary, not only because the systematics of Klebsiella has changed (Brisse et al 2014;Martínez et al 2004), but also because these strains differ in their virulence and antibiotic susceptibility (Podschun et al 2001). Accurate and rapid identification can facilitate the successful early resolution of infections and the subsequent reduction of morbidity and mortality.…”

Section: Introductionmentioning

confidence: 99%

Comparative analyses of phenotypic methods and 16S rRNA, khe, rpoB genes sequencing for identification of clinical isolates of Klebsiella pneumoniae

Guo

Shifei

et al. 2016

Antonie van Leeuwenhoek

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The present work aimed to evaluate 16S rRNA, khe and rpoB gene sequencing for the identification of Klebsiella pneumoniae in comparison with phenotypic methods. Fifteen clinical isolates were examined, which were initially identified as K. pneumoniae subsp. pneumoniae using the automated VITEK 32 system in two hospitals in Enshi City, China. Their identity was further supported by conventional phenotypic methods on the basis of morphological and biochemical characteristics. Using Bayesian phylogenetic analyses and haplotypes network reconstruction, 13 isolates were identified as K.pneumoniae, whereas the other two isolates (K19, K24) were classified as Shigella sp. and Enterobacter sp., respectively. Of the three genes, 16S rRNA and khe gene could discriminate the clinical isolates at the genus level, whereas rpoB could discriminate Klebsiella at the species and even subspecies level. Overall, the gene tree based on rpoB is more compatible with the currently accepted classification of Klebsiella than those based on 16S rRNA and khe genes, showing that rpoB can be a powerful tool for identification of K. pneumoniae isolates. Above all, our study challenges the utility of khe as a species-specific marker for identification of K. pneumoniae.Electronic supplementary material The online version of this article

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“…Klebsiella was highest in the Mooi and Vaal followed by Harts catchments with 19% and 11%, respectively. Podschun et al (2001) also reported a high percentage (53%) distribution of Klebsiella spp. from surface water samples, the most common species being K. pneumoniae.…”

Section: Resultsmentioning

confidence: 99%

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

Kinge¹,

Mbewe²,

Sithebe³

2012

Polymerase Chain Reaction

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Incidence of Klebsiella Species in Surface Waters and Their Expression of Virulence Factors

Cited by 168 publications

References 23 publications

Molecular Epidemiology of TwoKlebsiella pneumoniaeMastitis Outbreaks on a Dairy Farm in New York State

Molecular Epidemiology of TwoKlebsiella pneumoniaeMastitis Outbreaks on a Dairy Farm in New York State

Comparative analyses of phenotypic methods and 16S rRNA, khe, rpoB genes sequencing for identification of clinical isolates of Klebsiella pneumoniae

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

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