Incidence of Male-Killing<i>Rickettsia</i>spp. (α-Proteobacteria) in the Ten-Spot Ladybird Beetle<i>Adalia decempunctata</i>L. (Coleoptera: Coccinellidae)

Schulenburg, J. Hinrich Graf von der; Habig, Michael; Sloggett, John J.; Webberley, K. Mary; Bertrand, Dominique; Hurst, Gregory D. D.; Majerus, Michael E. N.

doi:10.1128/aem.67.1.270-277.2001

Cited by 95 publications

(80 citation statements)

References 45 publications

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“…To guard against this possibility we also sequenced mtDNA from A. decempunctata, which is the sibling species of A. bipunctata and is also infected with a malekilling Rickettsia (Lus 1947;Iablokoff-Khnzorian 1982;von der Schulenburg et al 2001). Mitochondrial sequences from the two species are known to form two monophyletic groups (von der Schulenburg et al 2002).…”

Section: Methodsmentioning

confidence: 99%

An Ancient Mitochondrial Polymorphism in Adalia bipunctata Linked to a Sex-Ratio-Distorting Bacterium

Jiggins

Tinsley

2005

Genetics

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Sex-ratio-distorting microbes are common parasites of arthropods. Although the reasons they have invaded and spread though populations are well understood, their subsequent dynamics within those populations are virtually unknown. We have found that different strains of a male-killing Rickettsia bacterium infecting the beetle Adalia bipunctata are associated with distinct mitochondrial haplotypes, which is expected as both the mitochondria and the bacteria are maternally transmitted. These mitochondrial haplotypes shared a common ancestor .2 million years ago, and their overall diversity is significantly greater than expected under neutrality from comparisons with a nuclear gene. Furthermore, a variety of statistical tests show strong deviations from neutrality in mitochondrial but not in nuclear genes. We therefore conclude that natural selection is probably maintaining a polymorphism of different Rickettsia strains in this species. Despite the age of the different mitochondrial haplotypes, there is very little genetic diversity within them. Furthermore, there is considerable variation in mitochondrial haplotype and bacterial strain frequency between populations, despite it being thought that this species has fairly low levels of population structure. We conclude that the fitness of these male killers may be negatively frequency dependent or different strains may be favored in different populations. These hypotheses await experimental confirmation.

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Section: Methodsmentioning

confidence: 99%

An Ancient Mitochondrial Polymorphism in Adalia bipunctata Linked to a Sex-Ratio-Distorting Bacterium

Jiggins

Tinsley

2005

Genetics

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“…In these cases, the Rickettsia bacterium is inherited by ovarial transmission through host generations with a high fidelity, is detected in host populations at a considerable frequency, and can be regarded as a facultative endosymbiotic associate (9,44). Thus far, such Rickettsia symbionts have been identified from a wide variety of invertebrates such as ladybird beetles (19,43,46), an aphid (6), a leafhopper (10), a bruchid beetle (13), ticks (2,30), leeches (21), and others.…”

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confidence: 99%

Rickettsia Symbiont in the Pea Aphid Acyrthosiphon pisum : Novel Cellular Tropism, Effect on Host Fitness, and Interaction with the Essential Symbiont Buchnera

Sakurai

Koga

Tsuchida

et al. 2005

Appl Environ Microbiol

225

248

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In natural populations of the pea aphid Acyrthosiphon pisum, a facultative bacterial symbiont of the genus Rickettsia has been detected at considerable infection frequencies worldwide. We investigated the effects of the Rickettsia symbiont on the host aphid and also on the coexisting essential symbiont Buchnera. In situ hybridization revealed that the Rickettsia symbiont was specifically localized in two types of host cells specialized for endosymbiosis: secondary mycetocytes and sheath cells. Electron microscopy identified bacterial rods, about 2 m long and 0.5 m thick, in sheath cells of Rickettsia-infected aphids. Virus-like particles were sometimes observed in association with the bacterial cells. By an antibiotic treatment, we generated Rickettsia-infected and Rickettsia-eliminated aphid strains with an identical genetic background. Comparison of these strains revealed that Rickettsia infection negatively affected some components of the host fitness. Quantitative PCR analysis of the bacterial population dynamics identified a remarkable interaction between the coexisting symbionts: Buchnera population was significantly suppressed in the presence of Rickettsia, particularly at the young adult stage, when the aphid most actively reproduces. On the basis of these results, we discussed the possible mechanisms that enable the prevalence of Rickettsia infection in natural host populations in spite of the negative fitness effects observed in the laboratory.

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“…The first report of molecular polymorphism in A. bipunctata is that of Schulenburg et al (2002). It is known that at least two species (A. bipunctata and A. decempunctata) are infected with symbiotic bacteria (Hurst et al, 1999;Von der Schulenburg et al, 2001). Under the term symbiosis are included any type of persistent biological interaction (ie mutualistic, commensalistic or parasitic).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of mtDNA in species of the genus Adalia (Coleoptera: Cocinellidae) and origin of ancient mitochondrial haplotypes in the gene pool of Adalia bipunctata

Захаров¹,

Шайкевич²

2013

Eur. J. Entomol.

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Abstract. Fifteen different mitochondrial haplotypes of the mtDNA gene COI encoding cytochrome C oxidase subunit I were identified in the 127 individuals of Adalia bipunctata studied. Two mitochondrial haplotypes, H9 and H10, differed greatly from the others. The mitochondrial polymorphism in A. bipunctata is ancient, though its age remains to be evaluated. It is shown that mitochondrial haplotypes H9 and H10 and others coexisted in the original population of A. bipunctata before it spread throughout Eurasia from Western Europe to the Baikal Area, and before the differentiation of the subspecies A. bipunctata fasciatopunctata, which differs from the European form in its elytral pattern. In order to evaluate the possible origin of the ancient mitochondrial haplotypes in the gene pool of A. bipunctata sequences of the mtDNA gene COI and of the rRNA second internal transcribed spacer of the four species of Adalia: A. bipunctata, A. decempunctata, A. frigida and A. tetraspilota, were compared. It is suggested that infection with Rickettsia had an important role in the preservation of the mitochondrial haplotypes H9 and H10 during the evolution of Adalia.

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Incidence of Male-KillingRickettsiaspp. (α-Proteobacteria) in the Ten-Spot Ladybird BeetleAdalia decempunctataL. (Coleoptera: Coccinellidae)

Cited by 95 publications

References 45 publications

An Ancient Mitochondrial Polymorphism in Adalia bipunctata Linked to a Sex-Ratio-Distorting Bacterium

An Ancient Mitochondrial Polymorphism in Adalia bipunctata Linked to a Sex-Ratio-Distorting Bacterium

Rickettsia Symbiont in the Pea Aphid Acyrthosiphon pisum : Novel Cellular Tropism, Effect on Host Fitness, and Interaction with the Essential Symbiont Buchnera

Comparative study of mtDNA in species of the genus Adalia (Coleoptera: Cocinellidae) and origin of ancient mitochondrial haplotypes in the gene pool of Adalia bipunctata

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