Incidence of methicillin resistant Staphylococcus aureus (MRSA) from septicemia suspected children

Saravanan, Muthupandian

doi:10.17485/ijst/2009/v2i12.5

Cited by 9 publications

(3 citation statements)

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“…In this study, all clinical MRSA strains (100%) were resistant to penicillin, rifampicin, ampicillin, gentamicin, and amoxacillin. A similar result was noted for gentamicin and penicillin in South India [15,16] and Tridad and Tobago (Southern Caribbean) [17].…”

Section: Discussionsupporting

confidence: 82%

Antibiotic Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus from Septicemia Suspected Children in Tertiary Hospital in Hosur, South India

Saravanan¹,

Nanda²,

Tesfaye³

2013

AJMR

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Septicemia is an important cause of morbidity and mortality among children in India. A wide variety of bacterial and fungal pathogen can cause septicemia. The objective of the present study is to determine the antibiotic susceptibility pattern of methicillin resistance Staphylococcus aureus from septicemia suspected children. A crosssectional study was undertaken from June, 2011 to August, 2012. A total of 300 children were screened. Study subjects were conveniently selected until the required sample size is full filled; blood samples were collected, transported and microbiologically processed using standard procedures; and data was cleaned and entered into a computer and statistical analysis was performed using SPSS for windows version 16. 88 (29.3%) were culture positive. S. aureus was the prominent isolate 26 (29.54 %). Of which 8(30.76% were methicillin resistant (MRSA). The present study reveals MRSA isolates were resistant to mostly all antibiotics and were sensitive to oxacillin and vancomycin.

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Section: Discussionsupporting

confidence: 82%

Antibiotic Susceptibility Pattern of Methicillin Resistant Staphylococcus aureus from Septicemia Suspected Children in Tertiary Hospital in Hosur, South India

Saravanan¹,

Nanda²,

Tesfaye³

2013

AJMR

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“…Similar resistance and sensitivity pattern has been demonstrated by many other studies also. [ 12 14 16 18 19 20 ] and 100% sensitivity has been reported to linezolid by yet another study. [ 18 ] However, least resistance of 4.8% has been reported in a study done among fishermen along the coastal area of Tamil Nadu in 2012.…”

Section: Discussionmentioning

confidence: 97%

Antimicrobial resistance pattern in a tertiary care hospital: An observational study

Saravanan

Raveendaran

2013

J Basic Clin Pharma

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Context:The number of organisms developing resistance to commonly used antibiotics is increasing among the various generations. The exact national scenario of antimicrobial resistance (AMR) is not known in India owing to the absence of a central monitoring agency.Aims:The aim of this study is to identify the group of organisms developing resistance, to know the classes of drugs against, which resistance has emerged and to assess the possible factors that can favor the development of AMR so that antibiotic policy can be formulated for the proper and effective use of antibiotics.Settings and Design:An observational study was conducted for a period of 1 year from August 2011 to July 2012 in a tertiary care hospital in Pondicherry.Subjects and Methods:Data regarding culture and sensitivity of the organisms isolated from different sources such as urine, blood, wound swab/pus, stool, sputum and tracheal aspirations were collected from the records of the Microbiology Department. Sample processing, identification of organisms to the genus and/or species level and antimicrobial sensitivity were carried out as per the Clinical and Laboratory Standards Institute guidelines on the 999 samples received.Results:Out of 999 samples, 125 (12.5%) showed significant growth of organisms exhibiting resistance to either single or multiple drugs. Out of 84 (67.2%) in-patients and 41 (32.8%) out-patient samples, Escherichia was the most common organism isolated with a total of 41 (32.8%), followed by Methicillin sensitive Staphylococcus aureus, 26 (20.8%), Klebsiella 25 (20%), Methicillin resistant Staphylococcus aureus 17 (13.6%), Pseudomonas 10 (8%), Proteus 2 (1.6%), 1 (0.8%) each of Citrobacter and Enterococci. Maximum resistance was observed with commonly used first line antimicrobials such as co-trimoxazole, ampicillin, amoxicillin, amoxyclav, fluoroquinolones, third generation cephalosporins and nalidixic acid. Least resistant or highly sensitive were amikacin, nitrofurantoin, gentamycin and doxycycline among the gram-negative bacteria. Macrolides, clindamycin, gentamycin, nitrofurantoin, vancomycin were the most sensitive antimicrobials against the gram-positive bacteria. Lack of knowledge on the consequences of inappropriate use of antibiotics was exhibited by 63% of subjects in our study.Conclusions:AMR was more with hospital acquired organisms and against commonly used antibiotics that are available since long period. Variation of resistance and sensitivity pattern with time and geographical location is identified. Periodic AMR monitoring and rotation of antibiotics are suggested to restrict further emergence of resistance.

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“…Biofilm formation also contributes to antibiotic resistance and tolerance to immune cells [ 7 ]. Methicillin-resistant S. aureus (MRSA), in particular, poses a greater risk, which causes septicemia in clinical settings [ 8 ]. The antibiotic-resistant isolates are hard to treat and a common cause of S. aureus -related mortalities.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Antimicrobial and Anti-Biofilm Activity of Natural Compounds and Their Analogues against Staphylococcus aureus Isolates

et al. 2022

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(1) Background: Staphylococcus aureus (S. aureus) is one of the most frequent causes of biofilm-associated infections. With the emergence of antibiotic-resistant, especially methicillin-resistant S. aureus (MRSA), there is an urgent need to discover novel inhibitory compounds against this clinically important pathogen. In this study, we evaluated the antimicrobial and anti-biofilm activity of 11 compounds, including phenyl propenes and phenolic aldehydes, eugenol, ferulic acid, sinapic acid, salicylaldehyde, vanillin, cinnamoyl acid, and aldehydes, against drug-resistant S. aureus isolates. (2) Methods: Thirty-two clinical S. aureus isolates were obtained from Alkhidmat Diagnostic Center and Blood Bank, Karachi, Pakistan, and screened for biofilm-forming potential, and susceptibility/resistance against ciprofloxacin, chloramphenicol, ampicillin, amikacin, cephalothin, clindamycin, streptomycin, and gentamicin using the Kirby-Bauer disk diffusion method. Subsequently, 5 representative clinical isolates were selected and used to test the antimicrobial and anti-biofilm potential of 11 compounds using both qualitative and quantitative assays, followed by qPCR analysis to examine the differences in the expression levels of biofilm-forming genes (ica-A, fnb-B, clf-A and cna) in treated (with natural compounds and their derivatives) and untreated isolates. (3) Results: All isolates were found to be multi-drug resistant and dominant biofilm formers. The individual Minimum Inhibitory Concentration (MIC) of natural compounds and their analogues ranged from 0.75–160 mg/mL. Furthermore, the compounds, Salicylaldehyde (SALI), Vanillin (VAN), α-methyl-trans-cinnamaldehyde (A-MT), and trans-4-nitrocinnamic acid (T4N) exhibited significant (15–92%) biofilm inhibition/reduction percentage capacity at the concentration of 1–10 mg/mL. Gene expression analysis showed that salicylaldehyde, α-methyl-trans-cinnamaldehyde, and α-bromo-trans-cinnamaldehyde resulted in a significant (p < 0.05) downregulation of the expression of ica-A, clf-A, and fnb-A genes compared to the untreated resistant isolate. (4) Conclusions: The natural compounds and their analogues used in this study exhibited significant antimicrobial and anti-biofilm activity against S. aureus. Biofilms persist as the main concern in clinical settings. These compounds may serve as potential candidate drug molecules against biofilm forming S. aureus.

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Incidence of methicillin resistant Staphylococcus aureus (MRSA) from septicemia suspected children

Cited by 9 publications

References 10 publications

Antibiotic Susceptibility Pattern of Methicillin Resistant <i>Staphylococcus </i><i>a</i><i>ureus</i> from Septicemia Suspected Children in Tertiary Hospital in Hosur, South India

Antibiotic Susceptibility Pattern of Methicillin Resistant <i>Staphylococcus </i><i>a</i><i>ureus</i> from Septicemia Suspected Children in Tertiary Hospital in Hosur, South India

Antimicrobial resistance pattern in a tertiary care hospital: An observational study

Analysis of the Antimicrobial and Anti-Biofilm Activity of Natural Compounds and Their Analogues against Staphylococcus aureus Isolates

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