Incidence of Multidrug-Resistant, Extensively Drug-Resistant, and Pandrug-Resistant Pseudomonas aeruginosa Strains Isolated from Clinical Specimens

Poudineh, Fatemeh; Azari, Ania Ahani; Fozouni, Leila

doi:10.29252/iem.6.3.211

Cited by 6 publications

(4 citation statements)

References 16 publications

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“…Different concentrations of the extract (25, 12.5, 6.25, 3.12 mg/ mL) were prepared by serial dilution method (14). The antibacterial effect of different concentrations of each extract against MDR P. aeruginosa isolates from our previous study (15) was determined by agar well diffusion and broth microdilution methods. In both methods, P. aeruginosa ATCC 27853 strain was used as a control.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva cylvestris, and Capsella bursa-pastoris against Multidrug-Resistant Pseudomonas aeruginosa Strains

Poudineh

Azari

Fozouni

2021

Avicenna J Clin Microbiol Infect

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Background: This study aimed to determine antibacterial activity of ethanolic extract of Matricaria chamomilla (chamomile), Malva sylvestris, and Capsella bursa-pastoris against multidrug-resistant (MDR) clinical isolates of Pseudomonas aeruginosa. Methods: The plants were collected from Ziarat village of Gorgan, Iran in April 2019. The required parts of the plants were separated and completely dried in the shade. After grinding, extraction was performed by maceration method. The extract was dried at 37°C for 24 hours. To obtain a concentration of 50 mg/mL of each extract, 500 mg of the dried plant extract was dissolved in 10 mL 5% dimethyl sulfoxide and sterilized by filtration through a 0.45 µm membrane filter. For the antibacterial assay, agar well diffusion and broth microdilution methods were used. Results: Based on the results, ethanolic extracts of M. sylvestris and Capsella bursa-pastoris did not show any antibacterial activity against MDR P. aeruginosa isolates in both antibacterial assays. No inhibitory effect was observed for ethanolic extract of chamomile against P. aeruginosa isolates in agar well diffusion method as well. In broth microdilution method, the extract of chamomile leaves showed inhibitory effect and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined as 12.5 and 25 mg/mL, respectively. Conclusions: In this study, the extract of ethanolic chamomile leaves showed antibacterial activity against the MDR P. aeruginosa isolates. Thus, it can be used in the production of antibacterial agents, and it is a good option for protection against pathogenic microorganisms, as well as P. aeruginosa.

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Section: Methodsmentioning

confidence: 99%

Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva cylvestris, and Capsella bursa-pastoris against Multidrug-Resistant Pseudomonas aeruginosa Strains

Poudineh

Azari

Fozouni

2021

Avicenna J Clin Microbiol Infect

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“…Pseudomonas aeruginosa belongs to the class of Gammaproteobacteria. XDR P. aeruginosa (XDR-PA) infections can have a high fatality rate, be extremely dangerous, and highly challenging to treat ( Ahani Azari and Fozouni, 2020 ). Mendes Pedro et al (2024) carried out a retrospective study on the patient records.…”

Section: Case Reportsmentioning

confidence: 99%

Extensively and multidrug-resistant bacterial strains: case studies of antibiotics resistance

Almutairy

2024

Front. Microbiol.

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The development of antibiotic resistance compromises the effectiveness of our most effective defenses against bacterial infections, presenting a threat to global health. To date, a large number of research articles exist in the literature describing the case reports associated with extensively drug-resistant (XDR) and multidrug-resistant (MDR) bacterial strains. However, these findings are scattered, making it time-consuming for researchers to locate promising results and there remains a need for a comparative study to compile these case reports from various geographical regions including the Kingdom of Saudi Arabia. Additionally, no study has yet been published that compares the genetic variations and case reports of MDR and XDR strains identified from Saudi Arabia, the Middle East, Central Europe, and Asian countries. This study attempts to provide a comparative analysis of several MDR and XDR case reports from Saudi Arabia alongside other countries. Furthermore, the purpose of this work is to demonstrate the genetic variations in the genes underlying the resistance mechanisms seen in MDR and XDR bacterial strains that have been reported in Saudi Arabia and other countries. To cover the gap, this comprehensive review explores the complex trends in antibiotic resistance and the growing risk posed by superbugs. We provide context on the concerning spread of drug-resistant bacteria by analyzing the fundamental mechanisms of antibiotic resistance and looking into individual case reports. In this article, we compiled various cases and stories associated with XDR and MDR strains from Saudi Arabia and various other countries including China, Egypt, India, Poland, Pakistan, and Taiwan. This review will serve as basis for highlighting the growing threat of MDR, XDR bacterial strains in Saudi Arabia, and poses the urgent need for national action plans, stewardship programs, preventive measures, and novel antibiotics research in the Kingdom.

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“…Different concentrations of the extract (25, 12.5, 6.25, 3.12 mg/mL) were prepared by serial dilution method [10] . Microorganisms: The antibacterial effect of each extract at different concentrations was assessed against MRSA (n=24) and MDR P. aeruginosa (n=16) isolates from our previous study [11][12] . In previous study, methicillin resistance was evaluated using 30 μg cefoxitin disk (≤21 mm indicated MRSA) and 1 μg oxacillin disk (≤10 mm indicated MRSA) [13] .…”

Section: Plant Materials and Preparation Of Extractsmentioning

confidence: 99%

“…A review of the bioactivity and potential ... [9] Izadi Z, Modarres Sanavi SAM, Sorooshzadeh A, Esna-Ashari M, Davoodi P. Antimicrobial activity of chamomile (Matricaria chamomilla L.) and feverfew (Tanacetum parthenium L.). Armaghane-Danesh J Yasuj .... [10] Imeni N, Qayami Rad M. In vitro antibacterial effect of ethanolic extract of Calendual officinalis L. against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas ... [11] Poudineh F, Ahani Azari A, Fozouni L. Incidence of multidrug-resistant, extensively ... [12] Sadat SS, Ahani Azari A. Frequency of multidrugresistant, extensively ...…”

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confidence: 99%

Antibacterial Effect of Matricaria chamomilla Alcoholic Extract against Drug-Resistant Isolates of Staphylococcus aureus and Pseudomonas aeruginosa

Azari

Danesh

2021

IEM

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Incidence of Multidrug-Resistant, Extensively Drug-Resistant, and Pandrug-Resistant Pseudomonas aeruginosa Strains Isolated from Clinical Specimens

Cited by 6 publications

References 16 publications

Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva cylvestris, and Capsella bursa-pastoris against Multidrug-Resistant Pseudomonas aeruginosa Strains

Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva cylvestris, and Capsella bursa-pastoris against Multidrug-Resistant Pseudomonas aeruginosa Strains

Extensively and multidrug-resistant bacterial strains: case studies of antibiotics resistance

Antibacterial Effect of Matricaria chamomilla Alcoholic Extract against Drug-Resistant Isolates of Staphylococcus aureus and Pseudomonas aeruginosa

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