Incidence of Multiple and Distinct Species of Caulimoviruses in Dahlia (Dahlia variabilis)

Abstract

<jats:p>Dahlia mosaic is a serious disease affecting dahlias. In addition to the <jats:italic>Dahlia mosaic virus</jats:italic> (DMV) reported previously, we characterized two putative new caulimoviruses, tentatively designated as DMV-D10 and Dahlia common mosaic virus (DCMV), from dahlia. To better understand their relative incidence in dahlia, a total of 213 samples were collected during 2007 and 2008 from several varieties of cultivated dahlia (<jats:italic>D. variabilis</jats:italic>) in the United States. Samples were tested for the three caulimoviruses using virus-specific primers in a polymerase chain reaction. Amplicons were cloned and sequenced to confirm the infection of dahlia with these viruses. Results showed that DMV-D10 was the most prevalent (94%) followed by DCMV (48.5%) and DMV (23%). Mixed infections were common and viruses were detected irrespective of symptom expression at the time of sampling. Two percent of the samples were not infected by any of the three tested caulimoviruses. Results suggest that caulimovirus infections are widespread in dahlia and highlight the need for testing and production of virus-free material to reduce their spread.</jats:p>

Identification and Genome Characterization of a Dahlia Common Mosaic Virus Isolate from China

“…Due to the fact that dahlia is usually propagated asexually by vegetative tuberous roots in cultivation, viral infections become a significant threat to dahlias [3]. Dahlia mosaic disease is a widespread and detrimental disease in dahlia with a variety of symptoms such as distortion, mosaic and chlorosis of leaves and overall stunting of plant [4,5]. Three caulimoviruses including dahlia mosaic virus (DMV), dahlia common mosaic virus (DCMV) and DMV-D10 have been reported to be associated with dahlia mosaic disease [6][7][8].…”

Plant pararetroviral sequences in wild Dahlia species in their natural habitats in Mexican mountain ranges

“…Tentatively referred to as Dahlia common mosaic virus (DCMV) and DMV-D10 (Pahalawatta et al, 2007;Pappu et al, 2008), these two new caulimoviruses showed significant sequence divergence compared to that of DMV (Pahalawatta et al, 2008b;Pappu et al, 2008). Both DCMV and DMV-D10 were found to be widespread based on surveys conducted in the USA and Europe (Pappu et al, 2005;Pahalawatta et al, 2007;Eid et al, 2009).…”