Incidence of Mycobacterium Avium Subspecies paratuberculosis in 
 Mehsani and Surti Goats of Indian Origin using Multiple Diagnostic Tests

Barad, D.B.; Chandel, B.S.; Dadawala, A.I.; Chauhan, H.C.; Kher, H.S.; Shroff, S.; Bhagat, A.G.; Singh, Shoor Vir; Singh, Pravin Kumar; Singh, A. V.; Sohal, J.S.; Gupta, Saurabh; Chaubey, Kundan Kumar; Chakrabort, Sandip; Tiwari, Ruchi; Deb, Rajib; Dhama, Kuldeep

doi:10.3923/jbs.2014.124.133

Cited by 9 publications

(3 citation statements)

References 23 publications

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“…High positivity of eld animals is attributed to the fact that these animals belonged to herds/farms with history of clinical incidences of paratuberculosis. Previous workers have also reported a high prevalence of paratuberculosis in the country [17][18][19][20]. This study is in agreement with previous studies wherein cattle were the most susceptible species for paratuberculosis [20][21].…”

Section: Resultssupporting

confidence: 92%

Comparative evaluation of various in-house protocols on diagnostic performance for paratuberculosis IS900 PCR

Gandhi

Jain²,

Gupta³

et al. 2022

Mol Biol Rep

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BackgroundParatuberculosis is a worldwide endemic infectious disease of ruminants that results in high economic losses.Public health concerns are also being raised with human Crohn's disease. Therefore, control is becoming priority for governments. Control is largely dependent on "Test and Cull" or "Test and Segregate" policy. Hence, it is critical to assure the infection before making the decision. Commercial kits are costly especially in view of resource limited areas. Present study analyzed the performance various in house DNA isolation methods and PCR master mix combinations to optimize a protocol for con rmation of paratuberculosis bacilli shedding in feces. Methods and ResultsPresent study included ve protocols of fecal DNA isolation (chemical, bio-chemical, physio-chemical and physical) and three reaction mixes (based on Qiagen, Genei and Thermo 2X master mixes) in nine different combinations using additives and tested their performance for IS900 PCR. Spiked fecal samples were used to select the best combination of DNA isolation method and PCR master mix (PRM). Selected combination was used to test reference (positive and negative) fecal samples and eld samples. Findings revealed that combination physical method of DNA isolation and Genei based PRM (with additives; betaine DMSO and BSA) had lowest limit of detection. Sensitivity was 83% and speci city was 100% in comparison to fecal culture. High prevalence (23%) was reported for paratuberculosis on eld samples. ConclusionOptimized protocol has acceptable sensitivity and can easily be adopted in resource-limited laboratories. High prevalence of paratuberculosis needs immediate implementation of the control strategies.

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Section: Resultssupporting

confidence: 92%

Comparative evaluation of various in-house protocols on diagnostic performance for paratuberculosis IS900 PCR

Gandhi

Jain²,

Gupta³

et al. 2022

Mol Biol Rep

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show abstract

“…The low percentage of MAP isolation in this study was also observed in others, such as Dimareli-Malli et al (2009);Medeiros et al (2012) and Barad et al (2014), which corroborates the low sensitivity of the isolation technique to individual paratuberculosis diagnosis. Furthermore, MAP from subclinical animals due to intermittent disposal and low number of bacteria in feces and milk (Whipple et al, 1991).…”

Section: Discussionsupporting

confidence: 92%

Molecular typing of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from dairy goats in Brazil

Souza

Lima

Braga

et al. 2016

Small Ruminant Research

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a b s t r a c tIn the last decade there was a significant increase dairy goat participation in the world and Brazilian agricultural scenario. Minas Gerais state is the third largest producer of goat milk in Brazil. Paratuberculosis is a chronic intestinal disease that mostly affects ruminants and can become a public health problem due to the possible association with Crohn's disease. It is transmitted by ingesting Mycobacterium avium subsp. paratuberculosis (MAP) contaminated food or water. In recent years, with the development of molecular techniques, the disease has been identified with greater precision and more quickly. MAP strains (S-Sheep, Cattle-C and B-Bison) have also been typed. The detection of a circulating MAP strain in a herd is important to develop epidemiological studies and thus establish better strategies to control paratuberculosis. This study aimed to identify and type MAP in dairy goat farms in the Zona da Mata, the main producing region of Minas Gerais state, Brazil. Feces and milk samples of 467 animals were collected, processed, inoculated in Herrold's Egg Yolk Agar (HEYM) and submitted to PCR and REA techniques. Eleven (2.36%) animals were positive for the presence of MAP in four properties and the isolates were characterized as type C strain. It was concluded that MAP is present in dairy goats from properties in Zona da Mata, MG and that strain type C circulates in the area. This is the first report of MAP typing isolated from dairy goat in Brazil.

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“…Recent advances in diagnostics, prophylaxis/vaccination alternative therapeutic modalities (Paolicchi et al, 2003;Tripathi et al, 2006;Kumar et al, 2007Kumar et al, , 2014Munjal et al, 2007;Deb et al, 2011; , 2013a,b,c,d, 2014Mahima et al, 2012;Barad et al, 2014) need to be explored fully in the era of emerging antibiotic resistance. Along with these follow up of appropriate/effective preventive and control measures are very crucial for alleviating high financial impacts due to this important pathogen of animals having zoonotic concerns keeping in view One Health, One Medicine approach (Grant, 2005;Tripathi et al, 2002;Momotani, 2012;Sweeney et al, 2012;Dhama et al, 2013e).…”

Section: Vaccinationmentioning

confidence: 99%

Recent Approaches in Diagnosis and Control of Mycobacterial Infections with Special Reference to Mycobacterium Avium Subspecies

Singh¹,

Sohal²,

Kumar³

et al. 2014

Adv. Anim. Vet. Sci.

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Incidence of Mycobacterium Avium Subspecies paratuberculosis in Mehsani and Surti Goats of Indian Origin using Multiple Diagnostic Tests

Cited by 9 publications

References 23 publications

Comparative evaluation of various in-house protocols on diagnostic performance for paratuberculosis IS900 PCR

Comparative evaluation of various in-house protocols on diagnostic performance for paratuberculosis IS900 PCR

Molecular typing of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from dairy goats in Brazil

Recent Approaches in Diagnosis and Control of Mycobacterial Infections with Special Reference to Mycobacterium Avium Subspecies

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