Incidence of virus diseases in maize fields in the Trakya region of Turkey

İlbağı, Havva; Rabenstein, Frank; Habekuß, Antje; Ordon, Frank; Çıtır, Ahmet

doi:10.7202/015853ar

Cited by 10 publications

(5 citation statements)

References 18 publications

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“…BYDVs adversely affect grain crops by reducing yield and quality all over the world (Perry et al, 2000;Rastgou et al, 2005), and infection by BYDV naturally can lead to 11-13% yield loss (Miller and Rasochová, 1997). Depending on the period of infection and the wheat variety, BYDVs produce noticeable common symptoms such as chlorosis, late heading, leaf reddening, and stunting in cereal crops (Yount et al, 1985;İlbağı et al, 2006). Similar to those previously reported, we observed symptoms evoke of BYDVs disease such as wheat plants with reddening at the leaf tips, dwarfed plants, yellowish lines on the leaves, and yellowed or dried patches in the fields surveyed in Şırnak Province in 2020 (Fig.…”

Section: Symptoms In Suspicious Wheat Plantssupporting

confidence: 86%

Current status and molecular phylogeny of some economically important viruses infecting wheat crops of Şırnak province, Turkey

Kapan,

Usta,

Güller

2023

Emir J Food Agric

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A survey of yellow dwarf viruses (YDVs) -associated diseases of wheat plants was performed for the first time in the Şırnak Province, Turkey. In plants wheat cultivated areas, symptoms such as diminished leaf size, dwarfing, yellowing, and reddening of leaves wheat plants were observed in cultivated areas. A total of 441 specimens were collected regardless of showing symptoms and assayed for Barley/Cereal Yellow Dwarf Viruses [B/CYDV] association by PCR tests using virus coat protein gene (CPG)-specific primer pairs. These assays demonstrated that 13.38% (55 samples) were positive to the occurrence of BYDV-PAV (9.73%) and CYDV-RPV (0.73%) for wheat viruses, but no BYDV-MAV, -SGV, and -RMV infections of BYDVs were found in PCR assays of collected samples. In addition, double infection of BYDV-PAV and CYDV-RPV was detected in 12 samples with a 2.91% infection rate. The CPG sequences of randomly selected two isolates were revealed using bacterial cloning and sequencing. Sequences obtained were deposited in GenBank under Accession numbers OL685734 and OL685736 for BYDV-PAV; and OL685735 and OL685737 for CYDV-RPV. BLASTn analysis of CPG sequences of four isolates shared high nucleotide homology with their intraspecies isolates, with 97.35-98.67% for two BYDV-PAV and 97.72-97.89 for two CYDV-RPV. The consensus tree constructed classified B/CYDV-associated wheat viruses with their phylogenetically closest individuals from Turkey and the world. This survey is the first report of BYDV-PAV and CYDV-RPV associated with wheat plants in Şırnak Province of Turkey. Keywords: BYDVs; Coat protein gene; Incidence; Sequencing; Şırnak

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Section: Symptoms In Suspicious Wheat Plantssupporting

confidence: 86%

Current status and molecular phylogeny of some economically important viruses infecting wheat crops of Şırnak province, Turkey

Kapan,

Usta,

Güller

2023

Emir J Food Agric

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“…Thus far, MDMV complete genome sequences have been reported in Bulgaria (Kong & Steinbiss, 1998), Spain (Achon et al, 2007), Hungary (Gell et al, 2010), Iran (Neishaburi et al, 2015), and the USA (Stewart et al, 2012; Wijayasekara & Ali, 2021). Also, MDMV was determined to be a widespread virus in multiple maize production regions in Turkey (Bremer & Raatikainen, 1975; Ilbağı et al, 2006). However, no sequence of an MDMV genome isolated from common reed has been determined.…”

Section: Figurementioning

confidence: 99%

High‐throughput sequencing of maize dwarf mosaic virus from common reed in a wetland

Ilbağı,

Masonbrink,

Miller

2023

Journal of Phytopathology

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Common reed is a widespread aquatic grass in wetland areas of Turkey. In reed samples, RNAs with the sequence of the Maize dwarf mosaic virus (MDMV) were found using high‐throughput sequencing. The nearly complete genome sequence comprised 9514 nucleotides. It contains a 5′ UTR of at least 145 nucleotides, a 3′ UTR of at least 237 nucleotides, and an ORF encoding a single polyprotein of 3041 amino acid residues. Two partial sequences were 7709 nt and 7858 nt long, respectively. Three reed sequences contained 22 nt, 25 nt, and 80 nt insertions in the 5′UTR, respectively. The coding region of MDMV TR‐34 (49%) was more conserved than UTRs (34.3%) compared to Johnsongrass mosaic virus (JGMV), Sugarcane mosaic virus (SCMV), and Sorghum mosaic virus (SrMV). MDMV‐TR34 reed isolate shares 91%–53.1% nucleotide identity and 98.2%–49% amino acid sequence similarity with other MDMV and three potyvirus sequences, respectively. Recombination analysis indicated six putative recombination events. In 2020–2022, the survey studies showed that 25 of 260 reed samples were infected with MDMV at the infection rate of 9.6% in Tekirdag province. This result is the first report of the complete nucleotidesequence of MDMV in the common reed, a reservoir host for cereals growing areas in Turkey.

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“…(1987) tarafından rapor edilmiştir. Ancak 2000 yılından itibaren Trakya Bölgesinde devam eden çalışmalar sonucunda başta buğday olmak üzere arpa, yulaf, tritikale, kuşyeminde YDVs'nden BYDV-PAV, BYDV-MAV, BYDV-SGV, BYDV-RMV ve CYDV-RPV saptanmıştır (İlbağı 2003(İlbağı , İlbağı et al 2003(İlbağı , Pocsai et al 2003(İlbağı , İlbağı ve Çıtır 2004a(İlbağı , 2004b(İlbağı , İlbağı et al 2005(İlbağı , İlbağı 2006(İlbağı , İlbağı et al 2006(İlbağı , İlbağı et al 2008. Ayrıca YDVs'nin yabancı ot konukçularından Phragmites communis'in özellikle BYDV-PAV için inokulum kaynağı olduğu bildirilmiştir (İlbağı 2006).…”

Section: Sonuçlar Ve Tartişmaunclassified

“…Tarla kenarlarındaki sarılık ve kızarıklık belirtisi sergileyen yabancı otların ise virüslerin inokulum kaynağı olarak varlığını sürdürdüğü YDVs'nin konukçusu yabancı ot türlerinden kamışlarda da aynı belirtiler gözlenmiştir (Şekil 2). 2000'li yıllardan itibaren başlayıp periyodik olarak günümüze kadar devam eden, Türkiye'nin 18 ilinde ve özellikle Trakya Bölgesindeki tahıl üretim alanlarında yaptığımız çalışmalarda YDVs'nin zaman zaman epidemilere neden olduğu belirlenmiştir (İlbağı 2003, İlbağı ve Çıtır 2004a, 2004b, İlbağı et al 2005, İlbağı 2006, İlbağı et al 2006, İlbağı et al 2008, İlbağı et al 2011, İlbağı ve Çıtır 2012, İlbağı 2013, İlbağı ve ark. 2013a, İlbağı et al 2013b, Hamamcı ve ark.…”

Section: Sonuçlar Ve Tartişmaunclassified

“…Türkiye ile aynı enlemler arasında ve ılıman iklim kuşağında yer alan Fransa'da, küresel iklim değişikliğinin özellikle Rhopalosiphum padi L. popülasyonlarında artışa neden olduğu; bu vektör türünün taşıdığı BYDV-PAV'ın epidemiler yaptığı; yaprakbiti tuzaklarında yakalanan vektör sayılarının BYDV-PAV virüs hastalık bulunuş oranları ile korelasyon içerisinde olduğu Fabre et al (2005) tarafından bildirilmiştir. 2000-2003 yıllarından itibaren Trakya Bölgesi tahıl üretim alanlarında periyodik olarak yapılan çalışmalarda YDVs hastalıklarının zaman zaman epidemilere neden olduğu belirlenmiştir (İlbağı 2003(İlbağı , İlbağı et al 2003(İlbağı , Pocsai et al 2003(İlbağı , İlbağı ve Çıtır 2004a(İlbağı , 2004b(İlbağı , İlbağı et al 2005(İlbağı , İlbağı 2006(İlbağı , İlbağı et al 2006(İlbağı , İlbağı et al 2008(İlbağı , İlbağı et al 2011(İlbağı , İlbağı ve Çıtır 2012(İlbağı , İlbağı 2013(İlbağı , İlbağı ve ark. 2013a(İlbağı , İlbağı et al 2013b(İlbağı , İlbağı ve ark.…”

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Tahıl üretim alanlarında sarı cücelik virüs hastalıkları (Yellow dwarf virus diseases) epidemisi ve mücadelesi

İlbağı¹

2017

Bitki Koruma Bülteni

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Epidemic of yellow dwarf virus diseases in cereal growing areas and their controlYellow dwarf virus (YDVs) diseases are one of the most important diseases which have reduced crop yield and quality in the cereal growing areas by causing epidemics from time to time on all over the world as well as in Turkey. Just as prevailing in some potential cereal producer provinces of Turkey as well as in Edirne, Kırklareli and Tekirdağ provinces of Trakya Region during the year of 2016, yellow dwarf virus epidemic diseases were taken place. Those virus diseases in cereal fields have become widespread especially in wheat fields which have reduced the yield and quality and caused economic losses. Survey studies were conducted and observation on YDVs disease epidemics in cereal fields especially in Edirne, Kırklareli and Tekirdağ provinces. At least 187 plant leaf samples were collected from the symptomatic winter bread wheat, barley and oat plants exhibiting yellowing, dwarfing, reddish symptoms and signs in the cereal growing areas. So, beside 138 bread wheat (Triticum aestivum L.), 19 barley (Hordeum vulgare L.) and 10 oat (Avena sativa L.) leaf samples, 20 samples were obtained from the perennial Poaceae weed host common reed (Phragmites austrialis (Cav.) Trin ex. Steudel). Barley yellow dwarf virus-PAV (BYDV-PAV), Barley yellow dwarf virus-MAV (BYDV-MAV) and Cereal yellow dwarf virus-RPV (CYDV-RPV) from YDVs were searched by employing double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) test. Reverse transcription polymerase chain reaction (RT-PCR) test was implemented for suspected leaf samples. As a result of DAS-ELISA and RT-PCR tests, 89 out of 187 leaf samples at the rate of 47.59% were found infected with BYDV-PAV. As 30 samples at the rate of 16.04% had CYDV-RPV and 4 of 187 leaf samples at rate of 2.14% were infected with BYDV-MAV viruses individually. Merely 14 out of 187 leaf samples at the rate of 48% were found infected with these tested YDVs (BYDV-PAV, BYDV-MAV and CYDV-RPV) as mixed infections. During the year of 2016, YDVs infections were determined at the highest level of incidence rate being 36.89% in the cereal growing areas of Edirne province. As in Kırklareli, YDVs incidence

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Incidence of virus diseases in maize fields in the Trakya region of Turkey

Cited by 10 publications

References 18 publications

Current status and molecular phylogeny of some economically important viruses infecting wheat crops of Şırnak province, Turkey

Current status and molecular phylogeny of some economically important viruses infecting wheat crops of Şırnak province, Turkey

High‐throughput sequencing of maize dwarf mosaic virus from common reed in a wetland

Tahıl üretim alanlarında sarı cücelik virüs hastalıkları (Yellow dwarf virus diseases) epidemisi ve mücadelesi

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