Incidence of Yersinia Enterocolitica in Farm Animals at Dakahlia and Kafr El-Sheikh Governorates

Abstract

Up to date little is known about incidence of Yersinia enterocolitica in farm animals in Egypt therefore this study was carried out. In this study, 200 samples were collected from diseased animals suffered from diarrhea including cows, buffaloes, sheep and goats were obtained from private and governorate farms at Dakahlia and Kafr El-Sheikh governorates. A total of 23 Y. enterocolitica strains were recovered. The prevalence rates were 14%, 14%, 10% and 8% in cows, sheep, Buffaloes and goats respectively. Y. enterocolitica isolates were biotyped, 21 isolates out of 23 were belonging to biotype 2 while,only 2 stains were belonging to biotype 1. Serotyping by using the available monovalent antisera, Y.enterocolitica were 19, 2, 2 strains belonging to O: 3, O: 5 and O: 9 serovars. Serotyping and biotypiny among different isolates revealed that 12 isolates O: 3/2, 4 isolates O: 5/2, 2 isolates O: 9/1 while 2 isolatesO: 9/2. As regard to the virulence tests, 13 out of tested strains were stable toxin strains, and 17 out of 23 strains were CRMOX producer positive but guinea pig conjunctivitis (Sereny test) was found to be negative for all examined serotypes. The PCR product was visualized after agarose gel electrophoresis. One amplification band of 591 bpwas observed when Y. enterocolitica colonies were used as template for the PCR. The serobiovars of Y. enterocolitica strains showing positive for virF gene were O: 3/2(2) and O: 9/2(1).