The objective of this study was to investigate the effects of a simulated futsal match protocol on isokinetic knee torque production. Twenty-one young futsal players participated in this study and performed a futsal-specific protocol comprising two blocks of 20-minute activities to simulate the match demands. At pre-protocol, half-time, and post-protocol, the concentric and eccentric isokinetic peak torque of the knee flexor and extensor muscles, the angle of peak torque, and the conventional and functional torque ratios were assessed. ANOVA was used to compare the variables (significance level p < 0.05). A decrease of knee flexor and extensor eccentric torque and knee flexor concentric torque was found, in which the pre-protocol levels were higher than those at half-time, with both being larger than those at post-protocol. The knee extensor concentric torque reduced at half-time. The angle of eccentric torque of knee flexors increased, and the conventional and functional torque ratios decreased at post-protocol. In conclusion, the protocol produced a time-dependent reduction of knee flexor and extensor torque in both concentric and eccentric actions. These findings suggested a possible impairment of performance and the emergence of risk factors for hamstring strains during a futsal match.