Inclusion by β-cyclodextrin of a pyrene-labeled dipeptide photoprobe

Jones, Guilford; Zhou, Xin; Lu, Lily N.

doi:10.1016/s0040-4039(02)01280-7

Cited by 4 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…K d values were determined from CD binding curves (Figure 4D and Figure 5D) using nonlinear regression analysis for a one-site-binding model. Stoichiometry ± SD was determined as described (Jones et al, 2002). …”

Section: Figuresmentioning

confidence: 99%

See 1 more Smart Citation

A Short Nur77-Derived Peptide Converts Bcl-2 from a Protector to a Killer

et al. 2008

Self Cite

View full text Add to dashboard Cite

SUMMARY Bcl-2 can be converted into a pro-apoptotic molecule by nuclear receptor Nur77. However, the development of Bcl-2 converters as anti-cancer therapeutics has not been explored. Here we report the identification of a Nur77-derived Bcl-2 converting peptide with 9 amino acids (NuBCP-9) and its enantiomer, which induce apoptosis of cancer cells in vitro and in animals. The apoptotic effect of NuBCPs and their activation of Bax are not inhibited but rather potentiated by Bcl-2. NuBCP-9 enantiomers bind to the Bcl-2 loop, which shares the characteristics of structurally adaptable regions with many cancer-associated and signaling proteins. NuBCP-9s act as molecular switches to dislodge the Bcl-2 BH4 domain, exposing its BH3 domain, which in turn blocks the activity of anti-apoptotic Bcl-XL.

show abstract

Section: Figuresmentioning

confidence: 99%

“…GST-Bcl-2 was titrated with NuBCP-9s in PBS, pH 7.6, 25°C at 219 nm where absorption from the free peptide is <0.3% of protein. Stoichiometry was determined as described (Jones et al, 2002). Data are presented as mean ± SD.…”

Section: Figuresmentioning

confidence: 99%

A Short Nur77-Derived Peptide Converts Bcl-2 from a Protector to a Killer

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…Without appropriate guest species, the fluorophore is partially residing in the hydrophobic cavity of the CD, whereas the presence of organic analytes leads to decomplexation of the fluorophore and a concomitant decrease of its fluorescence intensity. As compared with the parent CD, the chemically modified CDs can alter not only the original molecular binding ability but also the relative molecular selectivity significantly through further ligation or stereochemical complement of the functional sidearm located in the CD cavity [21][22][23][24]. Despite of the extensive interest, there are a number of limitations associated with the synthesis and purification of the modified CDs.…”

Section: Introductionmentioning

confidence: 99%