Inclusion of β-1,3/1,6-glucan in the ornamental fish, Jewel tetra (Hyphessobrycon eques), and its effects on growth, blood glucose, and intestinal histology

Furlan-Murari, Pâmela Juliana; Lima, Ed Christian Suzuki de; Souza, Felipe Pinheiro de; Urrea-Rojas, Angela Maria; Pupim, Andréia Carla Eugenio; Araújo, Eduardo José de Almeida; Meletti, Paulo César; Leal, Cindy Namie Seino; Fernandes, Letícia Lima; Lopera-Barrero, Nelson Maurício

doi:10.1007/s10499-021-00815-1

Cited by 4 publications

(4 citation statements)

References 51 publications

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“…Analysis of the number of goblet cells per intestinal crypt of the distal colon: The density of goblet cells (number of goblet cells per intestinal crypt) was determined by counting goblet cells in 40 intestinal crypts of the distal colon via periodic acid Schiff staining. Four histological sections per animal were analyzed with a Nikon 80i microscope with a 40 × objective[ 70 - 73 ]. Sections separated by an interval of 10 µm were analyzed, and crypts were chosen randomly.…”

Section: Methodsmentioning

confidence: 99%

Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

Magalhães¹,

Machado²,

Souza³

et al. 2023

World J Gastroenterol

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BACKGROUND The literature indicates that the enteric nervous system is affected in inflammatory bowel diseases (IBDs) and that the P2X7 receptor triggers neuronal death. However, the mechanism by which enteric neurons are lost in IBDs is unknown. AIM To study the role of the caspase-3 and nuclear factor kappa B (NF-κB) pathways in myenteric neurons in a P2X7 receptor knockout (KO) mouse model of IBDs. METHODS Forty male wild-type (WT) C57BL/6 and P2X7 receptor KO mice were euthanized 24 h or 4 d after colitis induction by 2,4,6-trinitrobenzene sulfonic acid (colitis group). Mice in the sham groups were injected with vehicle. The mice were divided into eight groups ( n = 5): The WT sham 24 h and 4 d groups, the WT colitis 24 h and 4 d groups, the KO sham 24 h and 4 d groups, and the KO colitis 24 h and 4 d groups. The disease activity index (DAI) was analyzed, the distal colon was collected for immunohistochemistry analyses, and immunofluorescence was performed to identify neurons immunoreactive (ir) for calretinin, P2X7 receptor, cleaved caspase-3, total caspase-3, phospho-NF-κB, and total NF-κB. We analyzed the number of calretinin-ir and P2X7 receptor-ir neurons per ganglion, the neuronal profile area (µm²), and corrected total cell fluorescence (CTCF). RESULTS Cells double labeled for calretinin and P2X7 receptor, cleaved caspase-3, total caspase-3, phospho-NF-κB, or total NF-κB were observed in the WT colitis 24 h and 4 d groups. The number of calretinin-ir neurons per ganglion was decreased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d groups, respectively (2.10 ± 0.13 vs 3.33 ± 0.17, P < 0.001; 2.92 ± 0.12 vs 3.70 ± 0.11, P < 0.05), but was not significantly different between the KO groups. The calretinin-ir neuronal profile area was increased in the WT colitis 24 h group compared to the WT sham 24 h group (312.60 ± 7.85 vs 278.41 ± 6.65, P < 0.05), and the nuclear profile area was decreased in the WT colitis 4 d group compared to the WT sham 4 d group (104.63 ± 2.49 vs 117.41 ± 1.14, P < 0.01). The number of P2X7 receptor-ir neurons per ganglion was decreased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d groups, respectively (19.49 ± 0.35 vs 22.21 ± 0.18, P < 0.001; 20.35 ± 0.14 vs 22.75 ± 0.51, P < 0.001), and no P2X7 receptor-ir neurons were observed in the KO groups. Myenteric neurons showed ultrastructural changes in the WT colitis 24 h and 4 d groups and in the KO colitis 24 h group. The cleaved caspase-3 CTCF was increased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d...

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Section: Methodsmentioning

confidence: 99%

Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

Magalhães¹,

Machado²,

Souza³

et al. 2023

World J Gastroenterol

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“…The concentrations of MacroGard® used were chosen because research using these concentrations has already obtained positive effects on productive performance, hematological, immunological, blood biochemical, and intestinal microbiota parameters (Aramli et al 2015, Do-Huu et al 2016, Ghaedi et al 2015, Harris et al 2020, Talpur et al 2014. The fixation of the additive in the feed was carried out based on the methodologies proposed by Furlan-Murari et al (2022) and Siwicki et al (2015).…”

Section: Diet Preparationmentioning

confidence: 99%

Effects of dietary β-glucans on the productive performance, blood parameters, and intestinal microbiota of angelfish (Pterophyllum scalare) juveniles

LIMA,

SOUZA,

FURLAN-MURARI

et al. 2024

An. Acad. Bras. Ciênc.

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Among the potential feed additives, β-glucans are known to positively affect the growth performance, blood parameters, and intestinal microbiota of fish, even the ornamental species. Therefore, the present study evaluated the effects of the dietary supplementation of different Saccharomyces cerevisiae β-glucans concentrations (0, 0.05, 0.1, and 0.2%) in juvenile angelfish (Pterophyllum scalare) over a 42-day period.Regarding growth performance, no effects were observed on most parameters. However, 0.2% β-glucans supplementation produced higher condition factor values, indicating a better nutritional status. Furthermore, β-glucans supplementation did not affect blood parameters. Regarding intestinal microbiota, β-glucans supplementation increased the abundance of the potentially beneficial bacterial genus Phascolarctobacterium.The high abundance of bacteria from the phylum Bacteroidetes, which can degrade β-glucans, may be attributed to the increased abundance of Phascolarctobacterium spp.In addition, 0.2% β-glucans supplementation produced more operational taxonomic units and higher Sobs (observed species richness), indicating effects on the overall bacterial community structure. These results demonstrate the potential application of β-glucans as a dietary supplement to improve the performance and modulate the intestinal microbiota of angelfish.

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“…In recent years, there has been a growing interest in determining the effects produced by prebiotic supplements in global fish aquaculture. The main prebiotics used include inulin [47,[49][50][51][52][53][56][57][58]61], β-glucans [30][31][32]34,36,37,40,69], FOS [50,61,70], MOS [52,61,65,69,70], GOS [43,61,[69][70][71], XOS [68,70], AXOS [29], IMO [45], and GroBiotic ® -A (International Ingredient Corporation, Lake City, MN, USA) [69] (Table 1).…”

Section: Use Of Prebiotics In Global Fish Aquaculturementioning

confidence: 99%

Prebiotics in Global and Mexican Fish Aquaculture: A Review

Amillano-Cisneros,

Fuentes-Valencia,

Leyva-Morales

et al. 2023

Animals

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Continued human population growth has resulted in increased demand for products, including those derived from aquaculture. The main challenge in aquaculture is producing more every year. In recent years, environmentally friendly supplements that provide the necessary pathways for optimal production have been emphasized. One of them is prebiotics, selectively utilized substrates by host microorganisms conferring a health benefit. Interest in applying prebiotics in global fish farming has increased in recent years as it has been shown to improve growth, boost the immune system, resist stress conditions, and cause the modulation of digestive enzymes. These effects reflect reduced production and disease costs. However, in Latin American countries such as Mexico, large-scale use of these food supplements is needed as a sustainable alternative to improve fish production. This paper gives a review of the current advances obtained with the application of prebiotics in commercially farmed fish worldwide, mentions the prebiotics to use in the aquaculture industry, and updates the status of studies about the used prebiotics in global commercial fish cultivated in Mexico, as well as freshwater and marine endemic fish in this country. Also, the limitations of prebiotics application in terms of their use and legislation are analyzed.

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Inclusion of β-1,3/1,6-glucan in the ornamental fish, Jewel tetra (Hyphessobrycon eques), and its effects on growth, blood glucose, and intestinal histology

Cited by 4 publications

References 51 publications

Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis

Effects of dietary β-glucans on the productive performance, blood parameters, and intestinal microbiota of angelfish (Pterophyllum scalare) juveniles

Prebiotics in Global and Mexican Fish Aquaculture: A Review

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