Water resources have become a barrier hampering socioeconomic development. Changes in the spatial distribution of water use profile have direct impacts to regional water use efficiency. However, the related research is inadequate, and a more suitable way to analyze regional water use profile is necessary. The Gini coefficient and location quotient, two reliable methods commonly used in discussing the spatial distribution of elements, were introduced and calculated. Taking Gansu Province as the study area, the concentration effect of industrial water use was the strongest, followed by domestic, environmental, and agricultural water use. However, a balanced distribution of agricultural water use failed to be coordinated with the production conditions, the center of Gansu’s grain production should be established to enhance the scale effect of agricultural production. The industrial agglomeration effect remained weak, and each region’s dominant industries need to be developed vigorously. Gansu’s domestic water use was consistent with its population distribution, but it is necessary to reduce the water use gap between urban and rural areas. With a growing awareness of environmental protection, environmental water use exhibited a more balanced distribution. Overall, the methods are simple and have good applicability, and the results can provide a reference in water use optimization.