Incoherent broad-band cavity-enhanced absorption spectroscopy

Fiedler, Sven E.; Hese, A.; Ruth, Albert A.

doi:10.1016/s0009-2614(03)00263-x

Cited by 313 publications

(272 citation statements)

References 70 publications

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“…those techniques that utilise broad-band emission sources, are increasing in popularity where it is desirable either to measure several compounds simultaneously that absorb in different spectral regions or where specificity is required for target species with extended absorption features (such as relatively large molecules or surface/liquid-borne species). Several studies have been reported that combine different broad-band sources such as arc-lamps [1][2][3][4][5][6] and light emitting diodes (LEDs) [7][8][9][10][11][12] with dispersive and interferometric detection schemes to detect gas phase species such as iodine oxides, 5 NO x compounds, 7,8,10,11 and solutions containing dyes (e.g. coumarin 334, brilliant blue-R, and rhodamine B and 6G) 9,12,13 which absorb at visible wavelengths.…”

Section: Introductionmentioning

confidence: 99%

Near-infrared broad-band cavity enhanced absorption spectroscopy using a superluminescent light emitting diode

Denzer

Hamilton

Hancock

et al. 2009

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A fibre coupled near-infrared superluminescent light emitting diode that emits $10 mW of radiation between 1.62 and 1.7 mm is employed in combination with a broad-band cavity enhanced spectrometer consisting of a linear optical cavity with mirrors of reflectivity $99.98% and either a dispersive near-infrared spectrometer or a Fourier transform interferometer. Results are presented on the absorption of 1,3-butadiene, and sensitivities are achieved of 6.1 Â 10 À8 cm À1 using the dispersive spectrometer in combination with phase-sensitive detection, and 1.5 Â 10 À8 cm À1 using the Fourier transform interferometer (expressed as a minimum detectable absorption coefficient) over several minutes of acquisition time.

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Section: Introductionmentioning

confidence: 99%

Near-infrared broad-band cavity enhanced absorption spectroscopy using a superluminescent light emitting diode

Denzer

Hamilton

Hancock

et al. 2009

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“…The BBCEAS measurements in a microtiter plate introduced some new experimental challenges. Initially, the alignment of the empty cavity was 6 optimised by iterative adjustment of the kinematic controls on the top and bottom cavity mirror mounts, such as to maximise the output reaching the detector. This procedure was repeated when an empty microtiter plate was introduced into the cavity by the adjustment of the 4 lockable mounts relative to the cavity mirrors.…”

Section: Methodsmentioning

confidence: 99%

“…This required only the measurement of the time integrated intensity exiting the cavity and so could use slower and cheaper detection setups but had the disadvantage of requiring calibration measurements to obtain the absorption coefficient of analytes. Further experimental innovations were demonstrated through broadband CEAS (BBCEAS) [6] , where a high intensity lamp was used as the light source instead of a laser and coupled with a multiplex detector to allow simultaneous measurement across a wide wavelength range. LED-BBCEAS has additionally reduced complexity and cost through the use of a high intensity LED as the light source [7] .…”

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confidence: 99%

Cavity-Enhanced Immunoassay Measurements in Microtiter Plates Using BBCEAS

et al. 2016

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We report on the first detailed use of broadband cavity enhanced absorption spectroscopy (BBCEAS) as a detection system for immunoassay. A vertical R ≥ 0.99 optical cavity was integrated with a motorized XY stage, which functioned as a receptacle for 96-well microtiter plates. The custom-built cavity enhanced microplate reader was used to make measurements on a commercially available osteocalcin sandwich ELISA kit. A 30-fold increase in path length was obtained with a minimum detectable change in the absorption coefficient, αmin(t), of 5.3 × 10–5 cm–1 Hz–1/2. This corresponded to a 39-fold increase in the sensitivity of measurement when directly compared to measurements in a conventional microplate reader. Separate measurements of a standard STREP-HRP colorimetric reaction in microtiter plates of differing optical quality produced an increase in sensitivity of up to 115-fold compared to a conventional microplate reader. The sensitivity of the developed setup compared favorably with previous liquid-phase cavity enhanced studies and approaches the sensitivity of typical fluorometric ELISAs. It could benefit any biochemical test which uses single pass absorption as a detection method, through either the label free detection of biologically important molecules at lower concentrations or the reduction in the amount of expensive biochemicals needed for a particular test, leading to cheaper tests.

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“…80 In IBBCEAS, an incoherent light source, such as a Xe-arc lamp, light emitting diode (LED) or supercontinuum laser, is continuously coupled into an optical cavity, consisting of two HR mirrors. Light transmitted through the cavity is dispersed by a grating onto a diode array or charge-coupled device (CCD) array.…”

Section: Introductionmentioning

confidence: 99%

Optomechanical Shutter Modulated Broad-Band Cavity–Enhanced Absorption Spectroscopy of Molecular Transients of Astrophysical Interest

et al. 2013

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We describe a sensitive spectroscopic instrument capable of measuring broad-band absorption spectra through supersonically expanding planar plasma pulses. The instrument utilizes incoherent broad-band cavityenhanced absorption spectroscopy (IBBCEAS) and incorporates an optomechanical shutter to modulate light from a continuous incoherent light source, enabling measurements of durations as low as ∼400 μs. The plasma expansion is used to mimic conditions in translucent interstellar clouds. The new setup is particularly applicable to test proposed carriers of the diffuse interstellar bands, as it permits swift measurements over a broad spectral range with a resolution comparable to astronomical observations. The sensitivity is estimated to be better than 10 ppm/pass, measured with an effective exposure time of only 1 s.

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Incoherent broad-band cavity-enhanced absorption spectroscopy

Cited by 313 publications

References 70 publications

Near-infrared broad-band cavity enhanced absorption spectroscopy using a superluminescent light emitting diode

Near-infrared broad-band cavity enhanced absorption spectroscopy using a superluminescent light emitting diode

Cavity-Enhanced Immunoassay Measurements in Microtiter Plates Using BBCEAS

Optomechanical Shutter Modulated Broad-Band Cavity–Enhanced Absorption Spectroscopy of Molecular Transients of Astrophysical Interest

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