Incomplete Simian Papovavirus Sv40

Abstract

The successful use of the fluoropyrimidines to gain information about the synthesis of viral DNA (1-3) led us to a study of their effect on the replication of vacuolating virus (SV40). This agent belongs to the papovavirus group 1 (4) which is characterized by 40 to 50 m/~ icosahedral particles possessing a shell of 42 capsomeres and a core of DNA (5, 6). As the time sequence of virus production in the one-step growth cycle had already been determined for SV40 (7), experiments could be readily designed with 5-fluorouracil (FU) and 5-fluorodeoxyuridine (FUDR) to determine whether the synthesis of infectious virus might be suppressed while the formation of viral proteins continued. Reissig and Kaplan (1) had shown that with another icosahedral DNAcontaining virus (swine herpes virus or pseudorabies) grown in the presence of FU, viral protein was assembled into empty particles having the size and shape of herpes virus but without DNA cores. In view of the well known tumorigenic activity of the papovaviruses, and the recent finding that SV40-induced tumors and transformed cells may contain complement-fixing viral antigen in the absence of infectious virus (8), information on the formation of viral antigens in cells which fail to make infectious virus is highly desirable. Materials and MethodsVirus.--The same frozen stock of virus that had been used for determining the growth cycle of SV40 (7) was employed. Its titer was 10 ~'5 plaque-forming units (PFU) per ml.

Demonstration of oncogenic potential of mammalian cells transformed by dna‐containing viruses following photodynamic inactivation

“…HSV virus-specific antigens and SV40 tumor antigen (T-antigen) were detected by the indirect immunofluorescence technique (Pope and Rowe, 1964;Melnick et al, 1964;Duff and Rapp, 1971a, b). SV40 virus antigen was detected by the direct immunofluorescence test (Melnick et al, 1964).…”

“…SV40 virus antigen was detected by the direct immunofluorescence test (Melnick et al, 1964). HSV membrane antigens were also detected by the indirect immunofluorescence test using unfixed cell preparations (Albrecht and Rapp, 1973).…”

The control of SV40 transcription during a lytic infection: late RNA synthesis in the presence of inhibitors of DNA replication

“…These late cytoplasmic RNAs probably represent the product of post-transcriptional processing (8,11). During a lytic infection in the presence of various inhibitors of viral and cellular DNA replication, the detection of only early viral RNA has generally been described (12)(13)(14)(15). More recent results supporting the observation that the control of late transcription is dependent on viral DNA replication were reported by Cowan et al (16).…”

Papovavirus SV40: Similarity of tumor antigens in transformed virus‐free hamster cells and in virus‐infected monkey cells

“…This antigen can be detected by complement fixation and by immunofluorescence techniques (Pope and Rowe, 1964;Rapp et al, 1964a), in sera from hamsters bearing tumors induced by the homologous virus. The same antigen has been found in cells of human and other species following transformation by SV40 Rapp et al, 1964a;Pope and Rowe, 1964;Sabin et al, 1964;Gilden et al, 1965;Habel et al, 1965) and has also been observed during the early phases of the cytolytic cycle of the virus in simian cells (Sabin and Koch, 1964;Rapp et al, 19646;Hoggan et al, 1965;Gilden et al, 1965). The antigen is soluble, thermolabile and serologically distinct from the SV40 capsid antigen Sabin and Koch, 1964;Gilden et al, 1965;Rapp et al, 1964a).…”