“…The tissular lipids were extracted according to Bligh & Dyer (1959), and the solvent soluble lipids were washed as already described (Carreau et al, 1977;Raulin & Grundt, 1980). Gasliquid chromatography was used to determine fatty acid composition of the total lipids extracted from the liver and brain, and to determine fatty acid composition of adipose-tissue phospholipids, after acetonic precipitation.…”