Incorporation of 14C-leucine into acid-insoluble material and tRNAleu from light stimulated rat brain

Abstract

Rats kept in the dark (i.e., less than 0.1 lux) from birth until 15 days were exposed to light. In vivo incorporation of 14C-leucine into acid insoluble material and in vitro aminoacylation of tRNAleu were studied in visual, somaesthetic and motor brain cortices. A significant increase of both 14C-leucine incorporation into acid insoluble material and tRNAleu aminoacylation with respect to the controls were found only in the visual cortex of light stimulated animals.

In vivo Cerebral Protein Synthesis Rates with Leucyl-Transfer RNA Used as a Precursor Pool: Determination of Biochemical Parameters to Structure Tracer Kinetic Models for Positron Emission Tomography

“…Structuring and assumptions applied to the tracer kinetic mod els (Hawkins et al, 1987(Hawkins et al, , 1989 from biochemical data presented here (Keen et al, 1987) will allow us to make accurate in vivo estimates of local brain reaction rates and kinetic rate constants when using noninvasive tracer techniques (Huang and Phelps, 1986;Sorenson and Phelps, 1987). Accurate mea surement of in vivo protein synthesis using tracer techniques and PET can provide a method for as sessing alterations in local protein synthesis rates associated with such processes as development (Barra et aI., 1972;Chou and Johnson, 1972;Harris and Maas, 1974;Srivastava et aI., 1984), regenera tion and repair in response to injury or disease (Yap and Spector, 1965;Bodsch et aI., 1986;Kiessling et aI., 1986), plasticity (Sayavedra, 1977), changes in duced by drugs and hormones (Carenzi and Casacci, 1977), degenerative disorders, neoplastic lesions (Barrio et aI., 1985), learning, and memory (Barondes, 1970;Robertson et aI., 1978;Schliebs et aI., 1985;Shashoua, 1985).…”