Incorporation of a Multi‐Valent Aptamer into Electrochemical Biosensors to Achieve an Improved Performance for Thrombin Analysis in Blood Serum

Zhu, Man; Xu, Fan; Miao, Siyuan; Xie, Chongyu; Li, Hui; Li, Shaoguang; Xia, Fan

doi:10.1002/cplu.202200325

Cited by 5 publications

(2 citation statements)

References 43 publications

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“…This sensor showed a linear detection range for thrombin in serum samples between 0.01 and 1000 nM. Zhu and co-workers took advantage of multivalent aptamers and developed an electrochemical thrombin sensor, which provides increased binding affinity and selectivity in target identification [68]. A fourfold increase in binding response was achieved for multivalent aptamers compared to aptamers consisting of a single recognition element.…”

Section: Electrochemical Sensors For Anticoagulants Using Thrombin-sp...mentioning

confidence: 99%

Electrochemical Monitoring in Anticoagulation Therapy

Mruthunjaya,

Torriero

2024

Molecules

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The process of blood coagulation, wherein circulating blood transforms into a clot in response to an internal or external injury, is a critical physiological mechanism. Monitoring this coagulation process is vital to ensure that blood clotting neither occurs too rapidly nor too slowly. Anticoagulants, a category of medications designed to prevent and treat blood clots, require meticulous monitoring to optimise dosage, enhance clinical outcomes, and minimise adverse effects. This review article delves into the various stages of blood coagulation, explores commonly used anticoagulants and their targets within the coagulation enzyme system, and emphasises the electrochemical methods employed in anticoagulant testing. Electrochemical sensors for anticoagulant monitoring are categorised into two types. The first type focuses on assays measuring thrombin activity via electrochemical techniques. The second type involves modified electrode surfaces that either directly measure the redox behaviours of anticoagulants or monitor the responses of standard redox probes in the presence of these drugs. This review comprehensively lists different electrode compositions and their detection and quantification limits. Additionally, it discusses the potential of employing a universal calibration plot to replace individual drug-specific calibrations. The presented insights are anticipated to significantly contribute to the sensor community’s efforts in this field.

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Section: Electrochemical Sensors For Anticoagulants Using Thrombin-sp...mentioning

confidence: 99%

Electrochemical Monitoring in Anticoagulation Therapy

Mruthunjaya,

Torriero

2024

Molecules

View full text Add to dashboard Cite

show abstract

“…This sensor showed a linear detection range for thrombin in serum samples between 0.01 and 1000 nM. Zhu and co-workers took advantage of multivalent aptamers, which provide increased binding affinity and selectivity in target identification and developed an electrochemical thrombin sensor [68]. A fourfold increase in binding response was achieved for multivalent aptamers compared to aptamers consisting of a single recognition element.…”

Section: Electrochemical Sensors For Anticoagulants Using Thrombin-sp...mentioning

confidence: 99%

Electrochemical Monitoring on Anticoagulation Therapy

Mruthunjaya,

Torriero

2024

Preprint

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The process of blood coagulation, wherein circulating blood transforms into a clot in response to internal or external injury, is a critical physiological mechanism. Monitoring this coagulation process is vital to ensure that blood clotting occurs neither too rapidly nor too slowly. Anticoagulants, a category of medications designed to prevent and treat blood clots, require meticulous monitoring to optimise dosage, enhance clinical outcomes, and minimise adverse effects. This review article delves into the various stages of blood coagulation, explores commonly used anticoagulants and their targets within the coagulation enzyme system, and emphasises the electrochemical methods employed in anticoagulant testing. Electrochemical sensors for anticoagulant monitoring are categorised into two types. The first type focuses on assays measuring thrombin activity via electrochemical techniques. The second type involves modified electrode surfaces that either directly measure the redox behaviour of anticoagulants or monitor the response of standard redox probes in the presence of these drugs. The review provides a comprehensive list of different electrode compositions and their detection and quantification limits. Additionally, it discusses the potential of employing a universal calibration plot to replace individual drug-specific calibrations. The insights presented are anticipated to significantly contribute to the sensor community's efforts in this field.

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Surface‐Based Multimeric Aptamer Generation and Bio‐Functionalization for Electrochemical Biosensing Applications

Hamidi,

Jahromi,

Hosseini

et al. 2024

Angewandte Chemie

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Multimeric aptamers have gained more attention than their monomeric counterparts due to providing more binding sites for target analytes, leading to increased affinity. This work attempted to engineer the surface‐based generation of multimeric aptamers by employing the room temperature rolling circle amplification (RCA) technique and chemically modified primers for developing a highly sensitive and selective electrochemical aptasensor. The multimeric aptamers, generated through surface RCA, are hybridized to modified spacer primers, facilitating the positioning of the aptamers in the proximity of sensing surfaces. These multimeric aptamers can be used as bio‐receptors for capturing specific targets. The surface amplification process was fully characterized, and the optimal amplification time for biosensing purposes was determined, using SARS‐CoV‐2 spike protein (SP). Interestingly, multimeric aptasensors produced considerably higher response signals and affinity (more than 10‐fold), as well as higher sensitivity (almost 4‐fold) compared to monomeric aptasensors. Furthermore, the impact of surface structures on the response signals was studied by utilizing both flat working electrodes (WEs) and nano‐/microislands (NMIs) WEs. The NMIs multimeric aptasensors showed significantly higher sensitivity in buffer and saliva media with the limit of detection less than 2 fg/ml. Finally, the developed NMIs multimeric aptasensors were clinically challenged with several saliva patient samples.

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Incorporation of a Multi‐Valent Aptamer into Electrochemical Biosensors to Achieve an Improved Performance for Thrombin Analysis in Blood Serum

Cited by 5 publications

References 43 publications

Electrochemical Monitoring in Anticoagulation Therapy

Electrochemical Monitoring in Anticoagulation Therapy

Electrochemical Monitoring on Anticoagulation Therapy

Surface‐Based Multimeric Aptamer Generation and Bio‐Functionalization for Electrochemical Biosensing Applications

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