“…[54][55][56][57][58] The initial aim of these studies was examination of the translation process. Cell-free systems with ribosomes isolated from various protozoan organisms( Tetrahymena pyriformis, [55] Crithidia oncopelti, [54,56] Paramecium aurelia, [57] Entamoeba histolytica [58] )w ere investigated to characterize the incorporation efficiency of amino acids depending on buffer supplements,p H, energy sources, cation concentrations, and supplementation with spermidine or other compounds. [54] More recently,acell-free system based on the protozoa Leishmania tarentolae [59] was established, in order to understand and characterizep arasitic proteins and their influence on parasitic biogenesis.…”