Incorporation of Astragalus polysaccharides injection during concurrent chemoradiotherapy in advanced pharyngeal or laryngeal squamous cell carcinoma: preliminary experience of a phase II double-blind, randomized trial

Hsieh, Ching-Hua; Lin, Chien‐Yu; Hsu, Cheng‐Lung; Fan, Kang‐Hsing; Huang, Shiang‐Fu; Liao, Chun‐Ta; Lee, Li‐Yu; Ng, Shu-Kung; Yen, Tzu‐Chen; Chang, Joseph Tung-Chieh; Lin, Jr-Rung; Wang, Hung‐Ming

doi:10.1007/s00432-019-03033-8

Cited by 28 publications

(11 citation statements)

References 24 publications

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“…Numerous polysaccharides and glycoproteins have been demonstrated to improve glucose consumption via several molecular pathways involved in glucose metabolism [ 45 ]. Some polysaccharides have been investigated in clinical trials, including resistant starch, Astragalus polysaccharide, or ginseng polysaccharide [ 46 , 47 , 48 ]. Our preliminary study showed that aquatic plant polysaccharides can promote insulin resistance.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Anh

Yang

2021

Polymers

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Polysaccharides are key bioactive compounds in lotus plumule tea, but their anti-diabetes activities remain unclear. The purpose of this study was to investigate the prebiotic activities of a novel polysaccharide fraction from the Nelumbo nucifera lotus plumule, and to examine its regulation of glucose metabolism in insulin-resistant HepG2 cells. The N. nucifera polysaccharide (NNP) was purified after discoloration, hot water extraction, ethanol precipitation, and DEAE-cellulose chromatography to obtain purified polysaccharide fractions (NNP-2). Fourier transform infrared spectroscopy was used to analyze the main structural characteristics and functional group of NNP-2. Physicochemical characterization indicated that NNP-2 had a molecular weight of 110.47 kDa and consisted of xylose, glucose, fructose, galactose, and fucose in a molar ratio of 33.4:25.7:22.0:10.5:8.1. The prebiotic activity of NNP-2 was demonstrated in vitro using Lactobacillus and Bifidobacterium. Furthermore, NNP-2 showed bioactivity against α-glucosidase (IC50 = 97.32 µg/mL). High glucose-induced insulin-resistant HepG2 cells were used to study the effect of NNP-2 on glucose consumption, and the molecular mechanism of the insulin transduction pathway was studied using RT-qPCR. NNP-2 could improve insulin resistance by modulating the IRS1/PI3K/Akt pathway in insulin-resistant HepG2 cells. Our data demonstrated that the Nelumbo nucifera polysaccharides are potential sources for nutraceuticals, and we propose functional food developments from the bioactive polysaccharides of N. nucifera for the management of diabetes.

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Section: Discussionmentioning

confidence: 99%

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Anh

Yang

2021

Polymers

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“…Most of studies have clari ed that APS inhibit tumor growth through immunopotentiation activity and generally be considered as a powerful adjunctive strategy in combination with chemotherapy for various tumors [15,16]. There are also parts of studies suggest the direct anti-tumor activity of APS, such as the inhibition of cell proliferation, the induction of cell apoptosis and cell cycle arrest [17,18].…”

Section: Discussionmentioning

confidence: 99%

Astragalus Polysaccharide Regulates EGFR and ANXA1 Expression to Inhibit Tumor Growth

Li¹,

Hu²,

Li³

et al. 2021

Preprint

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Background: Astragalus polysaccharide (APS) has been frequently used as an adjuvant agent responsible for its immunoregulatory activity to enhance efficacy and reduce toxicity of chemotherapy used in the management of breast cancer. However, the other synergism mechanism of APS remains unclear. Methods: The expression profiles of breast cancer biological data (E-GEOD-10780), (E-GEOD-65194), (E-GEOD-10810), (E-GEOD-42568), (E-GEOD-61304), (E-GEOD-7904) and (E-GEOD-15852) were retrieved from European bioinformatics institute (EBI) database to identify differentially expressed genes (DEGs). Gene Ontology and pathway enrichment analyses were carried out. The protein–protein interaction (PPI) networks of the DEGs were constructed by STRING online database. Finally, STRING was applied to construct the PPI networks of the DEGs associated with cell proliferation, cell cycle and cell apoptosis from GO enrichment analysis. Subsequently, hub and common genes that might be the potential targets and possible mechanism behind APS in vivo direct anti-tumor activity on breast cancer were identified. Meanwhile, we evaluated the mRNA and protein expression levels of these key targets using multiple biological analyses.Results: In total, 116 down-regulated and 73 up-regulated differential expressed genes (DEGs) were examined from seven gene expression datasets. Top ten hub genes were obtained in four typical protein-protein interaction (PPI) network of DEGs involved in each specific biological process (BP, cell cycle, cell proliferation, cell apoptosis and death) that was related to inhibitory activity of APS in vitro against breast cancer cell lines. Four common DEGs (EGFR, ANXA1, KIF14 and IGF1) were further identified in the above four BP-PPI networks, among which EGFR and ANXA1 were the hub genes that were potentially linked to the progression of breast cancer. The results of biological detections indicated that APS could down-regulated EGFR and up-regulated ANXA1 expression.Conclusion: In conclusion, the present study may provide potential molecular therapeutic targets and a new insight into the mechanism of APS against breast cancer.

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“…The mice were housed in a controlled environment with a temperature of 25±1˚C, relative humidity of 40-60%, a light/dark cycle of 12/12 h and free access to a standard diet and water. The mice were subcutaneously injected with 200 µl cancer cell suspension containing 6x10 6 AMC-HN-8 or AMC-HN-8-EVs1 cells at the right forelimb. At 5 days, the tumor formation rate was 100%.…”

Section: Methodsmentioning

confidence: 99%

“…He et al (1) have reported that age-standardized incidence rates of laryngeal carcinoma by Chinese standard population and by world standard population were 1.18 and 1.19 per 100,000 individuals, respectively, and age-standardized mortality rates by Chinese standard population and by world standard population were 0.61 and 0.61 per 100,000 individuals, respectively, in China in 2015. The conventional treatment options for laryngeal cancer include surgery (2,3), radiotherapy (4,5), chemotherapy (6,7) and biotherapy (8).…”

Section: Introductionmentioning

confidence: 99%

Effects of ATP-binding cassette transporter G2 in extracellular vesicles on drug resistance of laryngeal cancer cells in in vivo and in vitro

et al. 2021

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Drug resistance is one of the main factors limiting the efficacy of chemotherapy in patients with laryngeal cancer; thus, it is important to investigate the drug resistance of laryngeal cancer. In the present study, the mechanism of the regulation of drug resistance in laryngeal cancer cells by ATP-binding transporter G2 (ABCG2) that is present in the extracellular vesicles (EVs) released by drug-resistant cells was studied in vivo and in vitro.

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Incorporation of Astragalus polysaccharides injection during concurrent chemoradiotherapy in advanced pharyngeal or laryngeal squamous cell carcinoma: preliminary experience of a phase II double-blind, randomized trial

Cited by 28 publications

References 24 publications

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Astragalus Polysaccharide Regulates EGFR and ANXA1 Expression to Inhibit Tumor Growth

Effects of ATP-binding cassette transporter G2 in extracellular vesicles on drug resistance of laryngeal cancer cells in in vivo and in vitro

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