The current study was conducted to demonstrate the effect of using powdered (Asperjillius niger) in the diet of common carp (Cyprinus carpio). 72 fish were used, with an average weight of 3.24 g/fish, distributed randomly into three treatments, with three replicates for each treatment (6/fish / tank). Three diets were made with identical content and different addition percentages. The percentage of 33%, 66%, and 100% were used for treatments, T1, T2, and T3, respectively in addition to the compatriot treatment, which was devoid of Asperjillius. n powder. The fish were fed for 30 days with 4% of the biomass. The results indicated that the protease enzymes (Trypsin and Chymotrypsin) were distributed along digestive tract of the studied part (Anterior, middle, and Posterior) of the intestine. The results of statistical analysis also indicated that the value of the posterior part of the intestine differed significantly (P≤0.05) from those recorded in the Anterior and middle part of intestine. The three treatments showed the highest enzymatic activity (Trypsin and Chymotrypsin) after 30 days of feeding the fish, which differed significantly with the Posterior part of the intestine for all treatments, while the highest enzymatic activity was recorded in the middle part of the intestine. This study showed that (Aspergillius niger) can be substituted for soybean meal up to 100% without there being a negative effect on the enzymatic activity of the (Trypsin and Chymotrypsin) enzymes.