Incorporation of dimethyl sulfoxide into experimental hydrophilic and hydrophobic adhesive resins: evaluation of cytotoxic activities

Al-Ani, Anas Aaqel Salim Salim; Salim, Ikram Aqel; Seseogullari‐Dirihan, Roda; Mutluay, Mustafa Murat; Tjäderhane, Leo; Tezvergil‐Mutluay, Arzu

doi:10.1111/eos.12756

Cited by 4 publications

(2 citation statements)

References 44 publications

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“…68,69 This cell line was cultivated in DMEM High Glucose (DNAbiotech, Tehran, Iran) supplemented with 10% fetal bovine serum (FBS) (DNAbiotech, Tehran, Iran), 100 μg/mL of streptomycin, and 100 U/mL of penicillin (Biosera, Arya Tous, Tehran, Iran). A 95% humidity with a 5% CO 2 atmosphere was used to cultivate the cells at 37 C. 70…”

Section: Cell Culture Methodologymentioning

confidence: 99%

Rational peptide design for targeting cancer cell invasion

Gomari,

Arab,

Balalaie

et al. 2023

Proteins

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Cell invasion is an important process in cancer progression and recurrence. Invasion and implantation of cancer cells from their original place to other tissues, by disabling vital organs, challenges the treatment of cancer patients. Given the importance of the matter, many molecular treatments have been developed to inhibit cancer cell invasion. Because of their low production cost and ease of production, peptides are valuable therapeutic molecules for inhibiting cancer cell invasion. In recent years, advances in the field of computational biology have facilitated the design of anti‐cancer peptides. In our investigation, using computational biology approaches such as evolutionary analysis, residue scanning, protein–peptide interaction analysis, molecular dynamics, and free energy analysis, our team designed a peptide library with about 100 000 candidates based on A6 (acetyl‐KPSSPPEE‐amino) sequence which is an anti‐invasion peptide. During computational studies, two of the designed peptides that give the highest scores and showed the greatest sequence similarity to A6 were entered into the experimental analysis workflow for further analysis. In experimental analysis steps, the anti‐metastatic potency and other therapeutic effects of designed peptides were evaluated using MTT assay, RT‐qPCR, zymography analysis, and invasion assay. Our study disclosed that the IK1 (acetyl‐RPSFPPEE‐amino) peptide, like A6, has great potency to inhibit the invasion of cancer cells.

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Section: Cell Culture Methodologymentioning

confidence: 99%

Rational peptide design for targeting cancer cell invasion

Gomari,

Arab,

Balalaie

et al. 2023

Proteins

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“…Crucially, DMSO is classified as a class 3 solvent, boasting minimal toxicity and negligible risk to human health, similar to ethanol 26 . Consequently, the cytotoxicity of DMSO itself does not stand as a primary biological concern 27 . Despite its experimental applications in dentin treatment, there remains a dearth of information in the literature regarding the effectiveness of DMSO in the context of composite restoration repair.…”

Section: Introductionmentioning

confidence: 99%

Effects of different surface treatments on surface topography and bond strength in the repair of fiber‐reinforced dentin composite

Bayraktar,

Harorli

2024

J Esthet Restor Dent

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ObjectiveThis study aimed to evaluate the effects of different surface treatments on the repair bond strength between a fiber‐reinforced dentin composite and a posterior composite.MethodsForty fiber‐reinforced dentin composite resin blocks (4 mm × 4 mm × 4 mm) were separated into eight groups (n = 5) according to the surface preparation methods: (G1) negative control group, (G2) adhesive application, (G3) 50% dimethylsulfoxide (DMSO) application, (G4) 50% DMSO + adhesive application, (G5) 37% phosphoric acid etch + adhesive application, (G6) air abrasion + adhesive application, (G7) 37% phosphoric acid etch + 50% DMSO application + adhesive application, and (G8) air abrasion +50% DMSO application + adhesive application group. The composite surfaces were repaired in two layers with a posterior composite. Composite sticks were subjected to a micro tensile bond strength (μTBS) test. Fractured surfaces were evaluated using a stereomicroscope (×25). Short fiber‐reinforced composite samples' surfaces were investigated by scanning electron microscope (SEM). Shapiro Wilk, one‐way ANOVA, and Tukey HSD tests were used for statistical evaluation.ResultsThe highest average (μTBS) values were observed in G8, whereas the lowest mean μTBS values were evident in the G1 group. Statistically significant μTBS values were found in all adhesive‐applied groups when compared with the negative control group. Notably, the application of 50% DMSO without adhesive did not lead to a statistically significant increase in μTBS values. SEM images demonstrated that acid etching partially eliminated residues on the composite surface, while air abrasion had a detrimental effect on the integrity of fiber structures.ConclusionIn the repair of fiber‐reinforced dentin composite with a posterior composite, adhesive application is an effective approach. The treatment of 50% DMSO without adhesive did not confer a statistically significant advantage, and the supplemental use of acid etch or air abrasion did not show an additional benefit compared to adhesive‐only repairs.Clinical SignificanceAdhesive application emerges as a potent and effective strategy for the repair of bur‐roughened fiber‐reinforced dentin composites. With its limitations, the study highlights the efficacy of adhesive‐only repairs without the necessity for additional surface treatments.

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A critical review of the material properties guiding the clinician’s choice of root canal sealers

Cardinali

Camilleri

2023

Clin Oral Invest

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Objectives The introduction of hydraulic cement sealers has increased the popularity of single cone obturation where the chemistry and properties of hydraulic cement sealers are crucial. This article has investigated the materials present on the market by reviewing the chemistry aiming at understanding whether these materials are optimized or have been tested appropriately. Methodology A market search on materials called bioceramic and hydraulic sealers was undertaken. The safety data sheet and manufacturer details for every material were searched and the components were checked. The literature was searched for information about the properties of these materials based on their composition. Results The safety data sheets and manufacturer details were imprecise with some manufacturers providing little detail on composition. From the publications reviewed, it is apparent that the materials used clinically are not optimized, and there is little evidence that the material chemistry and presentation aid the clinical technique in any way. Conclusions There has been a rapid increase in materials identifying as bioceramics on the market. These materials have diverse chemistries, and some of the constituents are not declared. This may affect the clinical performance of these materials. Clinical significance Smart materials developed on the clinical need which are appropriately tested are necessary for a paradigm shift in root canal obturation. It is important to use reputable materials that have been adequately researched in clinical practice.

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Incorporation of dimethyl sulfoxide into experimental hydrophilic and hydrophobic adhesive resins: evaluation of cytotoxic activities

Cited by 4 publications

References 44 publications

Rational peptide design for targeting cancer cell invasion

Rational peptide design for targeting cancer cell invasion

Effects of different surface treatments on surface topography and bond strength in the repair of fiber‐reinforced dentin composite

A critical review of the material properties guiding the clinician’s choice of root canal sealers

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