Incorporation of exudates of human platelet‐rich fibrin gel in biodegradable fibrin scaffolds for tissue engineering of cartilage

Chien, Chi-Sheng; Ho, Hsiu O.; Liang, Yu Chih; Ko, Pai Hung; Sheu, Ming Thau; Chen, Chien Ho

doi:10.1002/jbm.b.32657

Cited by 54 publications

(36 citation statements)

References 58 publications

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“…Akeda and colleagues [29] reported that 10% PRP treatment significantly increased proteoglycan and Col II synthesis compared to treatments with 10% PPP or 10% FBS, with the major profiles of proteoglycan and collagens being similar to those seen in cells cultured with FBS, indicating maintenance of a stable chondrocyte phenotype with PRP exposure. Similar effects were also noted in human osteoarthritic chondrocytes [30]. The supernatant from platelet-rich fibrin (PRF) up-regulated the mRNA expression of Col II and aggrecan and increased synthesis of glycosaminoglycan and proteoglycan by chondrocytes cultured either on the two-dimensional surface of fibrin scaffolds or in three-dimensional scaffolds compared to controls without addition of exudate.…”

Section: Effect Of Platelet-rich Plasma On Cellsmentioning

confidence: 61%

“…When adult porcine chondrocytes were cultured in alginate beads in the presence of 10% PRP releasate (PRPr), 10% platelet-poor plasma (PPP) releasate or 10% fetal bovine serum (FBS) for 72 hours, the increase in DNA content in the PRP group was significantly higher than that in the PPP group and that in the FBS group [29]. The stimulatory effect of PRP on chondrocyte proliferation was also observed after 4, 8 and 12 days of in vitro culture, in either a monolayer or three-dimensional environment [30]. Additional studies revealed a dose- and time-dependent enhancement of chondrocyte proliferation by PRPr, even during the 20 day monolayer culture [31,32].…”

Section: Effect Of Platelet-rich Plasma On Cellsmentioning

confidence: 99%

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Biology of platelet-rich plasma and its clinical application in cartilage repair

2014

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Platelet-rich plasma (PRP) is an autologous concentrated cocktail of growth factors and inflammatory mediators, and has been considered to be potentially effective for cartilage repair. In addition, the fibrinogen in PRP may be activated to form a fibrin matrix to fill cartilage lesions, fulfilling the initial requirements of physiological wound healing. The anabolic, anti-inflammatory and scaffolding effects of PRP based on laboratory investigations, animal studies, and clinical trials are reviewed here. In vitro, PRP is found to stimulate cell proliferation and cartilaginous matrix production by chondrocytes and adult mesenchymal stem cells (MSCs), enhance matrix secretion by synoviocytes, mitigate IL-1β-induced inflammation, and provide a favorable substrate for MSCs. In preclinical studies, PRP has been used either as a gel to fill cartilage defects with variable results, or to slow the progression of arthritis in animal models with positive outcomes. Findings from current clinical trials suggest that PRP may have the potential to fill cartilage defects to enhance cartilage repair, attenuate symptoms of osteoarthritis and improve joint function, with an acceptable safety profile. Although current evidence appears to favor PRP over hyaluronan for the treatment of osteoarthritis, the efficacy of PRP therapy remains unpredictable owing to the highly heterogeneous nature of reported studies and the variable composition of the PRP preparations. Future studies are critical to elucidate the functional activity of individual PRP components in modulating specific pathogenic mechanisms.

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Section: Effect Of Platelet-rich Plasma On Cellsmentioning

confidence: 61%

Section: Effect Of Platelet-rich Plasma On Cellsmentioning

confidence: 99%

Biology of platelet-rich plasma and its clinical application in cartilage repair

2014

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“…In fact, there are studies where fibrin and agarose scaffolds are used for cartilage regeneration. 29 Fibrin and FA hydrogels could also be used for the regeneration of damaged skin or as subcutaneous adipose tissue, even though the value of the viscoelastic moduli of skin tissues (G 0 2 MPa) or subcutaneous adipose tissue (G 0 104 Pa; G 00 103 Pa) are higher than the ones measured for FA. 20 Again, the viscoelastic moduli of FA hydrogels could probably be increased above the values reported in this work by increasing the agarose content.…”

Section: Effect Of Agarose Contentmentioning

confidence: 99%

Effect of the hydration on the biomechanical properties in a fibrin‐agarose tissue‐like model

Scionti

Moral

Toledano

et al. 2013

J Biomedical Materials Res

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The effect of hydration on the biomechanical properties of fibrin and fibrin-agarose (FA) tissue-like hydrogels is reported. Native hydrogels with approximately 99.5% of water content and hydrogels with water content reduced until 90% and 80% by means of plastic compression (nanostructuration) were generated. The biomechanical properties of the hydrogels were investigated by tensile, compressive, and shear tests. Experimental results indicate that nanostructuration enhances the biomechanical properties of the hydrogels. This improvement is due to the partial draining of the water that fills the porous network of fibers that the plastic compression generates, which produces a denser material, as confirmed by scanning electron microscopy. Results also indicate that the characteristic compressive and shear parameters increase with agarose concentration, very likely due to the high water holding capacity of agarose, which reduces the compressibility and gives consistency to the hydrogels. However, results of tensile tests indicate a weakening of the hydrogels as agarose concentration increases, which evidences the anisotropic nature of these biomaterials. Interestingly, we found that by adjusting the water and agarose contents it is possible to tune the biomechanical properties of FA hydrogels for a broad range, within which the properties of many native tissues fall.

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“…It has been shown that the concentrations of growth factors, proteins and lipids in PRFr are higher than those in supernatant serum . In addition, the PRFr up‐regulated the mRNA expressions of type II collagen aggrecan and increased synthesis of glycosaminoglycan and proteoglycan in human OA chondrocytes . Therefore, we hypothesized that PRFr may serve as a mixture of growth factors and improve injury repair.…”

Section: Introductionmentioning

confidence: 98%

“…25 In addition, the PRFr up-regulated the mRNA expressions of type II collagen aggrecan and increased synthesis of glycosaminoglycan and proteoglycan in human OA chondrocytes. 26 Therefore, we hypothesized that PRFr may serve as a mixture of growth factors and improve injury repair. In this study, we investigated the therapeutic effects of PRFr in combination with MSCs for articular cartilage regeneration in a rabbit model.…”

Section: Introductionmentioning

confidence: 99%

Intra‐articular Injection of platelet‐rich fibrin releasates in combination with bone marrow‐derived mesenchymal stem cells in the treatment of articular cartilage defects: An in vivo study in rabbits

Sheu

Hsu

et al. 2016

J Biomed Mater Res

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The use of mesenchymal stem cells (MSCs), which can be differentiated into chondrocytes under specific conditions, has been proposed for the treatment of cartilage defects. Blood-derived platelet-rich fibrin releasate (PRFr), which is rich in growth factors and cytokines, may improve cartilage regeneration. In this study, the therapeutic effects of PRFr in combination with bone marrow-derived MSCs for articular cartilage regeneration were evaluated in a rabbit model. Critical osteochondral defects were surgically created in the femoral condyle of the rabbits, and 3 × 10 of MSCs, 0.8 mL of PRFr, or a combination of MSCs and PRFr were injected intra-articularly and one week after first administration. The animals were sacrificed 12 weeks postoperatively, and the regenerated cartilages were assessed by gross inspection and histological examination. No treatment-related adverse events were noted in any of the rabbits. The size of the defect decreased and the volume of regenerated cartilage increased in the medial femoral condyles of the MSCs + PRFr group. Relative to the MSCs or PRFr group, histological examination demonstrated that the MSCs + PRFr group had thicker hyaline-like cartilaginous tissue with normal glycosaminoglycan production. Grading scores revealed that MSCs + PRFr injection had better matrix, cell distribution, and surface indices than other groups. The results showed that intra-articular injections of MSCs + PRFr into the knee can reduce cartilage defects by regenerating hyaline-like cartilage without adverse events. This approach may provide an alternative method of autologous chondrocyte implantation to repair cartilage defects with an unlimited source of cells and releasate. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1536-1543, 2017.

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Incorporation of exudates of human platelet‐rich fibrin gel in biodegradable fibrin scaffolds for tissue engineering of cartilage

Cited by 54 publications

References 58 publications

Biology of platelet-rich plasma and its clinical application in cartilage repair

Biology of platelet-rich plasma and its clinical application in cartilage repair

Effect of the hydration on the biomechanical properties in a fibrin‐agarose tissue‐like model

Intra‐articular Injection of platelet‐rich fibrin releasates in combination with bone marrow‐derived mesenchymal stem cells in the treatment of articular cartilage defects: An in vivo study in rabbits

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