Incorporation of fixed oils into spearmint oil-loaded nanoemulsions and their influence on characteristic and cytotoxic properties against human oral cancer cells

Tubtimsri, Sukannika; Limmatvapirat, Chutima; Limsirichaikul, Siripan; Akkaramongkolporn, Prasert; Piriyaprasarth, Suchada; Patomchaiviwat, Vipaluk; Limmatvapirat, Sontaya

doi:10.1016/j.jddst.2021.102443

Cited by 16 publications

(34 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, silica nanoparticles (SNPs) loaded with linalool and α-pinene contributed to the life-extension of both terpenes when incubated at 45 °C for 180 days 56 . Likewise, Tubtimsri et al (2021) showed that the nanoemulsified spearmint oil presented a carvone (main compound in this essential oil) retention over 90% when kept at 40 °C for 180 days 57 .…”

Section: Resultsmentioning

confidence: 90%

Comprehensive study of α-terpineol-loaded oil-in-water (O/W) nanoemulsion: interfacial property, formulation, physical and chemical stability

et al. 2021

View full text Add to dashboard Cite

In this study, the interfacial ability of α-terpineol (α-TOH) was reported, followed by its trapping into oil-in-water (O/W) nanoemulsion as active-ingredient and the long-term observation of this nanosystem influenced by the storage-time (410-days) and temperature (5, 25, 50 °C). The results indicated that the α-TOH can reduce the interfacial tension on the liquid-liquid interface (ΔG°m = −1.81 KJ mol−1; surface density = 8.19 × 10−6 mol m−2; polar head group area = 20.29 Å2), in the absence or presence of surfactant. The O/W nanoemulsion loaded with a high amount of α-TOH (90 mg mL−1; 9α-TOH-NE) into the oil phase was successfully formulated. Among the physical parameters, the mean droplet diameter (MDD) showed a great thermal dependence influenced by the storage-temperature, where the Ostwald ripening (OR) was identified as the main destabilizing phenomena that was taking place on 9α-TOH-NE at 5 and 25 °C along with time. Despite of the physical instability, the integrity of both nanoemulsion at 5 °C and 25 °C was fully preserved up to 410th day, displaying a homogeneous and comparable appearance by visual observation. On contrary, a non-thermal dependence was found for chemical stability, where over 88% of the initial amount of the α-TOH nanoemulsified remained in both 9α-TOH-NE at 5 and 25 °C, up to 410th day. Beyond the key data reported for α-TOH, the importance of this research relies on the long-term tracking of a nanostructured system which can be useful for scientific community as a model for a robust evaluation of nanoemulsion loaded with flavor oils.

show abstract

Section: Resultsmentioning

confidence: 90%

Comprehensive study of α-terpineol-loaded oil-in-water (O/W) nanoemulsion: interfacial property, formulation, physical and chemical stability

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The primer sequences for Bax were 5′-GAGCAGATCATGAAGACAGG-3′ (forward) and 5′-GGCGGCAATCATCCTCTG-3′ (reverse); for Bcl-2, 5′-GACTTCCCGCCGCTACC-3′ (forward) and 5′-CTTCACTTGTGGCCCAGATAGG-3′ (reverse); and for 18 s rRNA, 5′-AATCAGGGTTCGATTCCGGA-3′ (forward) and 5′-CCAAGATCCAACTACGAGCT-3′ (reverse). The relative mRNA level was calculated as follows ( n = 3) [ 17 ]: ΔCt = Ct target − Ct ref ΔΔCt = ΔCt treatment − ΔCt untreated Relative gene expression level (fold change) = 2 − ΔΔCt …”

Section: Methodsmentioning

confidence: 99%

“…Crosslinking proteins, such as sodium caseinate and transglutaminase, at the oil–water interface prior to the emulsification process significantly reduced the ripening rate of alkane nanoemulsions during extended storage periods. Moreover, the incorporation of hydrophobic compounds with poor water solubility, such as Aerosil 200 [ 15 ], fixed oil [ 17 , 18 ], or rosin gum [ 15 ], in the internal phase delays Ostwald ripening [ 11 , 19 ]. Nonetheless, few studies have demonstrated the efficacy of Ostwald ripening inhibitors, such as fixed oil and polyethylene glycol (PEG 4000), on the formation and properties of nanoemulsions, particularly AMO nanoemulsions, using a PIT method.…”

Section: Introductionmentioning

confidence: 99%

Impact of Fixed Oil on Ostwald Ripening of Anti-Oral Cancer Nanoemulsions Loaded with Amomum kravanh Essential Oil

et al. 2022

Self Cite

View full text Add to dashboard Cite

Recently, essential oil from Amomum kravanh (AMO) was reported to exert anti-oral cancer effects. Although it was more effective after being loaded into nanoemulsions, AMO without an Ostwald ripening inhibitor was unable to form stable nanoemulsions because of the Ostwald ripening phenomenon. In this study, we examined the influence of Ostwald ripening inhibitors, such as fixed oils and polyethylene glycol 4000 (PEG 4000), on nanoemulsion properties prepared by a phase inversion temperature method. Several fixed oils, including virgin coconut oil (VCO), palm oil (PMO), olive oil (OLO), and PEG 4000, were evaluated, and their Ostwald ripening inhibitory effects were compared. The results suggest that the type and ratio of AMO:fixed oils influence the formation and characteristics of nanoemulsions. PEG 4000 was unable to produce nanoemulsions; however, stable nanoemulsions with small droplet sizes were observed in preparations containing OLO and VCO at an AMO:fixed oil ratio of 80:20, which may be the result of specific molecular interactions among the components. Using an MTT assay, we demonstrated that the AMO:OLO (80:20) nanoemulsion produced the most significant cytotoxic effect on oral cancer cells with a percentage of 99.68 ± 0.56%. Furthermore, the AMO:OLO 80:20 nanoemulsion inhibits metastasis and induces oral cancer cell death through the intrinsic apoptosis pathway. In conclusion, AMO nanoemulsion with anti-oral cancer activity was successfully produced by varying the amount and type of fixed oils. In the future, this discovery may lead to the development of stable nanoemulsions employing additional volatile oils.

show abstract

“…This meant that the Massoia lactone contained in the NEs still kept high antifungal activity. In contrast, the activity of spearmint oil that inhibits oral cancer cells can be increased when it is incorporated into the NEs [30]. Indeed, the oil in water NEs containing cinnamaldehyde, eugenol and carvacrol have significant antifungal activity against P. digitatum spore germination, mycelial morphology and membrane permeability and can be used for prolonging the storage period of citrus fruits [16].…”

Section: Anti-fungal Activity Of the Massoia Lactone-loaded Nesmentioning

confidence: 99%

Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast

Zhang

Chi

et al. 2022

JMSE

View full text Add to dashboard Cite

Nanoemulsions (NEs) have been made for improving the delivery and disperse of bioactive compounds. In this study, it was found that the best ingredients for the stable Massoia lactone-loaded and food-grade NEs making were 560.0 µL of Tween-80, 240.0 µL of Span-80 and 200.0 µL of Massoia lactone. Then, 9.0 mL of distilled water was titrated into the mixture under continuous magnetic stirring (750 rotations min−1) with about 2 drops per second for 20 min. Finally, the system was treated by ultrasonication using an ultrasonic generator (180 W and 22 KHz) for 5 min. All the prepared particles with a mean droplet diameter of 43 nm were spherical, had uniform size distribution and were equally distributed in the Massoia lactone-loaded NEs. The obtained Massoia lactone-loaded nanoemulsions (NEs) were very stable without changes of the mean droplet diameter and polydispersity indexes (PDI) for over two months under different conditions. As with free Massoia lactone, Massoia lactone loaded in the NEs had high anti-fungal activity against Metschnikowia bicuspidate LIAO, a pathogenic yeast causing milky disease in the Chinese mitten crab by damaging its cell membrane and causing cellular necrosis. Massoia lactone loaded in the NEs also had the DPPH radical scavenging activity and the hydroxyl radical scavenging activity.

show abstract

Incorporation of fixed oils into spearmint oil-loaded nanoemulsions and their influence on characteristic and cytotoxic properties against human oral cancer cells

Cited by 16 publications

References 36 publications

Comprehensive study of α-terpineol-loaded oil-in-water (O/W) nanoemulsion: interfacial property, formulation, physical and chemical stability

Comprehensive study of α-terpineol-loaded oil-in-water (O/W) nanoemulsion: interfacial property, formulation, physical and chemical stability

Impact of Fixed Oil on Ostwald Ripening of Anti-Oral Cancer Nanoemulsions Loaded with Amomum kravanh Essential Oil

Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast

Contact Info

Product

Resources

About