Incorporation of Heavy Water for Rapid Detection of Salmonella typhimurium by Raman Microspectroscopy

Feng, Jingjing; Yao, Weirong; Guo, Yahui; Cheng, Yuliang; Qian, He; Xie, Yunfei

doi:10.1007/s12161-018-1328-5

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…In addition to chemical pollution, biological contaminants, including microorganisms and their toxic metabolites, viruses, parasites and their eggs, and vector insects, pose food safety risks. Among these, mold and mycotoxin contamination are the most common [ 31 , 32 ]. Unlike vegetables and fruits, grains and nuts require long-term storage facilities, and are more suitable for storage because of their lower water content.…”

Section: Sers Application In “From Farm To Table” Foodsmentioning

confidence: 99%

Innovative Application of SERS in Food Quality and Safety: A Brief Review of Recent Trends

Gao

Han

et al. 2022

Foods

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Innovative application of surface-enhanced Raman scattering (SERS) for rapid and nondestructive analyses has been gaining increasing attention for food safety and quality. SERS is based on inelastic scattering enhancement from molecules located near nanostructured metallic surfaces and has many advantages, including ultrasensitive detection and simple protocols. Current SERS-based quality analysis contains composition and structural information that can be used to establish an electronic file of the food samples for subsequent reference and traceability. SERS is a promising technique for the detection of chemical, biological, and harmful metal contaminants, as well as for food poisoning, and allergen identification using label-free or label-based methods, based on metals and semiconductors as substrates. Recognition elements, including immunosensors, aptasensors, or molecularly imprinted polymers, can be linked to SERS tags to specifically identify targeted contaminants and perform authenticity analysis. Herein, we highlight recent studies on SERS-based quality and safety analysis for different foods categories spanning the whole food chain, ‘from farm to table’ and processing, genetically modified food, and novel foods. Moreover, SERS detection is a potential tool that ensures food safety in an easy, rapid, reliable, and nondestructive manner during the COVID-19 pandemic.

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Section: Sers Application In “From Farm To Table” Foodsmentioning

confidence: 99%

Innovative Application of SERS in Food Quality and Safety: A Brief Review of Recent Trends

Gao

Han

et al. 2022

Foods

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“…Because deuterium has been shown to have a growth-inhibiting effect on bacteria, 22,28 concentrations of 20−50% D 2 O (with respect to the normal water content) are typically used for bacteria growth, but reports of growth using up to 100% of D 2 O exist. 21,27,28,31,32 In 2015, Berry et al presented the first study using Raman spectroscopy to detect the antibiotic effect on bacteria using deuterium labeling. 22 Here, we leverage the sensitivity of Raman-based AST in combination with stable isotope labeling to advance this technique beyond the classical yes/no (resistant/susceptible) answer.…”

mentioning

confidence: 99%

“…Deuterium incorporation by replacing a certain amount of water (H 2 O) in the cultivation medium by heavy water (D 2 O) leads to a more random and untargeted deuterium incorporation; however, a high amount of deuterium can be introduced and detected by Raman spectroscopy. Because deuterium has been shown to have a growth-inhibiting effect on bacteria, , concentrations of 20–50% D 2 O (with respect to the normal water content) are typically used for bacteria growth, but reports of growth using up to 100% of D 2 O exist. ,,,, In 2015, Berry et al presented the first study using Raman spectroscopy to detect the antibiotic effect on bacteria using deuterium labeling …”

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confidence: 99%

Heteroresistant Bacteria Detected by an Extended Raman-Based Antibiotic Susceptibility Test

et al. 2020

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Worldwide, multiresistant bacterial strains are emerging at unprecedented rates. This development seriously threatens the ability of humanity to treat even common infections, resulting in disability and death. Furthermore, this development endangers all medical achievements including cancer therapy or organ transplantations. Therefore, the World Health Organization has endorsed antimicrobial resistance as a great threat to humanity. To still allow effective treatment of patients, rapid, automated, and reliable antibiotic susceptibility testing (AST) of bacterial pathogens is essential. Thereby, speed and sensitivity of the AST results are crucial for improving patient care. Here, Raman spectroscopy as a nondestructive technique providing chemical-specific information is employed to monitor the deuterium uptake of metabolically active bacteria during antibiotic treatment, enabling fast and reliable AST. For this purpose, a bulk sample-preparation method was developed, allowing a high-throughput analysis of a significant number of cells. A protocol was developed for Gram-positive (Enterococcus faecalis) and Gram-negative (Escherichia coli) reference strains and was tested on 51 clinical isolates with wellcharacterized resistance phenotypes against ampicillin, ciprofloxacin, meropenem, and vancomycin. Borderline resistant and heteroresistant phenotypes were observed and further investigated. This is of critical importance as the sensitive detection of lowfrequency heteroresistance in bacterial populations is a huge challenge. Such isolates seem susceptible but are resistant to treatment in vivo. Automatable analysis detects strong phenotypes within 3 h. On the basis of experimental and modeled data, heteroresistance is estimated to be detectable down to frequencies of 10 −6 and investigated on clinical isolates as a proof-of-concept study, but requiring longer incubation time.

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Ramanome technology platform for label-free screening and sorting of microbial cell factories at single-cell resolution

Wang

et al. 2019

Biotechnology Advances

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Incorporation of Heavy Water for Rapid Detection of Salmonella typhimurium by Raman Microspectroscopy

Cited by 5 publications

References 21 publications

Innovative Application of SERS in Food Quality and Safety: A Brief Review of Recent Trends

Innovative Application of SERS in Food Quality and Safety: A Brief Review of Recent Trends

Heteroresistant Bacteria Detected by an Extended Raman-Based Antibiotic Susceptibility Test

Ramanome technology platform for label-free screening and sorting of microbial cell factories at single-cell resolution

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