Incorporation of isotope from specifically labeled glucose into alginates of Pseudomonas aeruginosa and Azotobacter vinelandii

Lynn, A R; Sokatch, John R.

doi:10.1128/jb.158.3.1161-1162.1984

Cited by 40 publications

(19 citation statements)

References 14 publications

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“…Several Gram-negative, aerobic microorganisms that are related to the pseudomonads appear to utilize the Entner-Doudoroff pathway in the cyclic mode. It has been shown that incorporation of labelled glucose into alginate by Azotobacter vinelandii involves recycling of triose phosphates [60,61]. In Alcaligenes eutrophus, phosphoglycerate mutase mutants lose the ability to grow on succinate, but are still able to grow on fructose, a pattern similar to that observed for Pseudomonas mutants of the triose phosphate pathway [62].…”

Section: 'Cycfic' Pathwaymentioning

confidence: 73%

The Entner-Doudoroff pathway: history, physiology and molecular biology

Conway¹

1992

FEMS Microbiology Letters

321

194

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The Entner‐Doudoroff pathway is now known to be very widely distributed in nature. Biochemical and physiological studies show that the Entner‐Doudoroff pathway can operate in a linear and catabolic mode, in a ‘cyclic’ mode, in a modified mode involving non‐phosphorylated intermediates, or in alternative modes involving C1 metabolism and anabolism. Molecular and genetic analyses of the Entner‐Doudoroff pathway in Zymomonas mobilis, Escherichia coli and Pseudomonas aeruginosa have led to an improved understanding of some fundamental aspects of metabolic controls. It can be argued that the Entner‐Doudoroff pathway is more primitive than Embden‐Meyerhof‐Parnas glycolysis.

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Section: 'Cycfic' Pathwaymentioning

confidence: 73%

The Entner-Doudoroff pathway: history, physiology and molecular biology

Conway¹

1992

FEMS Microbiology Letters

321

194

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“…During atginate synthesis. GDP-D-mannose is formed following enzymatic conversion starting with fnjctose-6-phosphate, derived from the Entner-Doudoroff pathway (Lynn and Sokatch. 1984).…”

Section: Discussionmentioning

confidence: 99%

Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: a role for guanosine diphospho (GDP)‐D‐mannose

Lightfoot

Lam

1993

Molecular Microbiology

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Pseudomonas aeruginosa can express two distinct forms of lipopolysaccharide (LPS), called A-band and B-band. As an attempt to understand the molecular biology of the synthesis and regulation of these LPS antigens, a recombinant plasmid, pFV3, containing genes for A-band expression was isolated previously. In the present study, P. aeruginosa strain PAO1 was mutagenized with transposon Tn5-751 and yielded a B-band-deficient mutant, called ge6. This mutant was mated with a PAO1 genomic library, and transconjugants were screened for complementation of B-band using B-band-specific monoclonal antibody MF15-4. Recombinant plasmid pFV100 was subsequently isolated by its ability to complement B-band expression in ge6. SDS-PAGE analysis of LPS from ge6 and ge6(pFV100) revealed that ge6 was deficient in expression of B-band, while ge6(pFV100) had an LPS profile similar to that of the parent strain PAO1. With A-band and B-band genes cloned in separate plasmids, pFV3 and pFV100 respectively, we were able to determine the map location of these LPS genes on the P. aeruginosa PAO1 chromosome using pulsed-field gel electrophoresis. A-band genes mapped at 5.75 to 5.89 Mbp (SpeI fragment SpK; DpnI fragment DpF2), while genes involved with expression of B-band LPS mapped at 1.9 Mbp (SpeI fragments SpC, SpI and SpAI; DpnI fragment DpD) on the 5.9 Mbp chromosome. We also performed initial characterization of a gene involved with synthesis of A-band present on pFV3. We previously reported that recombinant plasmid pFV3 and subcloned plasmid pFV36 complemented A-band synthesis in rd7513, an A- mutant derived from A+ strain AK1401. pFV36 was mutagenized with transposon Tn1000 to reveal a one-kilobase region capable of complementing the expression of A-band in the A- strain rd7513. This region was subcloned as a 1.6 kb KpnI fragment into plasmid vector pAK1900 and the resulting clone named pFV39. Labelling of proteins encoded by pAK1900 and pFV39 in Escherichia coli maxicells revealed a single unique polypeptide of approximately 37 kDa expressed by pFV39. Supernatants from disrupted cells of rd7513(pFV39) and AK1401 converted 14C-labelled-guanosine diphospho (GDP)-D-mannose to GDP-rhamnose, while supernatants from rd7513 did not show synthesis of GDP-rhamnose. The data therefore suggest that conversion of GDP-D-mannose to GDP-rhamnose is required for synthesis of A-band LPS, and that a 37 kDa protein is involved in this conversion.

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“…Synthesis starts with the entry of six carbon substrates into the Entner–Doudoroff pathway, resulting in pyruvate, which is channelled towards the tricarboxylic acid cycle. Subsequently, oxaloacetate is converted to fructose‐6‐phosphate via gluconeogenesis (Lynn and Sokatch, ; Narbad et al ., ). Three well‐characterized enzymes, AlgA, AlgC and AlgD, catalyse the four next biosynthesis steps to convert fructose‐6‐phosphate to GDP‐mannuronic acid and these enzymes have been.…”

Section: Microbial Biosynthesis Of Alginatementioning

confidence: 99%

Microbial alginate production, modification and its applications

Hay

Rehman

Moradali

et al. 2013

Microbial Biotechnology

271

173

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Alginate is an important polysaccharide used widely in the food, textile, printing and pharmaceutical industries for its viscosifying, and gelling properties. All commercially produced alginates are isolated from farmed brown seaweeds. These algal alginates suffer from heterogeneity in composition and material properties. Here, we will discuss alginates produced by bacteria; the molecular mechanisms involved in their biosynthesis; and the potential to utilize these bacterially produced or modified alginates for high-value applications where defined material properties are required.

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Incorporation of isotope from specifically labeled glucose into alginates of Pseudomonas aeruginosa and Azotobacter vinelandii

Cited by 40 publications

References 14 publications

The Entner-Doudoroff pathway: history, physiology and molecular biology

The Entner-Doudoroff pathway: history, physiology and molecular biology

Chromosomal mapping, expression and synthesis of lipopolysaccharide in Pseudomonas aeruginosa: a role for guanosine diphospho (GDP)‐D‐mannose

Microbial alginate production, modification and its applications

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