Incorporation of Label from 13C-, 2H-, and 15N-Labeled Methionine Molecules during the Biosynthesis of 2[prime]-Deoxymugineic Acid in Roots of Wheat

Identification, expression analysis and molecular modeling of Iron deficiency specific clone 3 (Ids3) like gene in hexaploid wheat

“…In the present study, we reported that Ids3 gene, which encodes a dioxygenase that converts DMA to MA or MA to epiHMA, is present in hexaploid wheat. These results are contrary to a number of earlier reports showing absence of the Ids3 gene(s) or presence of a mere pseudo gene in hexaploid wheat and its wild relatives (Nakanishi et al 2000; Mori et al 1990; Ma and Nomoto 1994) The bioinformatics analysis indicated that the functional wheat ortholog of Ids3 gene might be present at chromosome 7AS, which is supported by the results obtained from physical mapping of the wheat ortholog in the telomeric region of chromosome 7AS. In the constructed phylogenetic tree, the close evolutionary relationship of TaIds3_7AS with HvIds3 also supports the above results (Fig.…”

“…Our results are also supported by the location of most of the QTLs of high grain iron and zinc contents on chromosome 7A of different Triticum species (Tiwari et al 2009; Peleg et al 2009). The Ids3 gene has been mapped on long arm of chromosome 4H of barley and long arm of chromosome 5R of rye using wheat-barley addition lines and wheat-rye addition lines respectively (Ma and Nomoto 1994; Mori and Nishizawa 1989; Ma et al 1999).…”

Incorporation of14C into methionine as an intermediate for phytosiderophore biosynthesis in barley

“…The l4C distribution into the other amino acids, Glu + Asn and Ala, did not decrease. Therefore, it is suggested that l4C may be incorporated into MAs via Met, because all the 3 amino-butanoic-acid units of the compounds in MAs were derived from the carbon skeleton of Met (Kawai et al 1988;Ma and Nomoto 1994). It is also suggested that the Met "pool" for the formation of MAs could be mixed with external Met and may not be isolated in the root cells.…”

“…Information on the interconversion (Mori and Nishizawa 1987;Kawai et al 1988;Ma and Nomoto 1994) is well documented. Based on our knowledge, data about the metabolic rate of Met in iron-deficient roots are necessary to confirm the precursory role of Met.…”

“…Several authors (Mori and Nishizawa 1987;Kawai et al 1993;Mori 1994;Ma and Nomoto 1994;Ma et al 1995) carried out extensive studies on the biosynthesis of MAs and reported that Met fed externally is converted to MAs. Higuchi et al (1996) and Higuchi et al (1999) investigated the pathway between methionine (Met) and MAs, and the related enzymes and genes.…”