Incorporation of Label into Pollen Tube Walls from Myoinositol-labeled <i>Lilium longiflorum</i> Pistils

Kroh, M.; Miki-Hirosige, H.; Rosen, Walter G.; Loewus, Frank A.

doi:10.1104/pp.45.1.92

Cited by 72 publications

(34 citation statements)

References 2 publications

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“…The decrease is probably due to the uptake of the canal exudate by growing pollen tubes. This is supported by the findings that 14C was incorporated into pollen tubes growing in the canal exudate when the pistil was administered 14C-myo-inositol or 14C-glucose (Kroh et al, 1970;Labarca and Loewus, 1973), and that canal exudate injected into stylar canal promoted the growth of incompatible pollen tubes proportionate to the increase in carbohydrate concentration (Fig. 2).…”

Section: Discussionsupporting

confidence: 73%

Changes in the Amount and Composition of Stylar Canal Exudate after Self- or Cross-pollination in Self-incompatible Lilium longiflorum Thunb.

Ichimura

Yamamoto

1992

Engei Gakkai zasshi

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Section: Discussionsupporting

confidence: 73%

Changes in the Amount and Composition of Stylar Canal Exudate after Self- or Cross-pollination in Self-incompatible Lilium longiflorum Thunb.

Ichimura

Yamamoto

1992

Engei Gakkai zasshi

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“…At the height of canal exudate production, 5 days after anthesis, 2% of the "C was present in that exudate. In experiment 2, 4 days after anthesis, 3.4% of the "C was in stigmatic exudate and 1.6% in canal exudate.…”

Section: Methodsmentioning

confidence: 99%

The Nutritional Role of Pistil Exudate in Pollen Tube Wall Formation in Lilium longiflorum

Labarca¹,

Loewus²

1973

Plant Physiol.

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117

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Detached pistils of Lilium longiflorum were labeled with D-glucose-U-'4C 24 hours after anthesis and then sampled for the next 6 days to determine the appearance of label into exudate from the stylar canal and the stigmatic surface of the pistil. Results were obtained with unpollinated cv. Ace pistils and pollinated cv. Ace pistils, selfed or crossed (cv. Croft pollen). Limited data were also obtained on cv. Croft pistils, selfed or crossed (cv. Ace pollen).Exudate appeared in the canal and on the stigmatic surface soon after anthesis. In unpollinated pistils it continued to accumulate for about 5 days in the canal and for the full term of the experiment, 7 days, on the stigmatic surface. Canal exudate eventually mixed with stigmatic exudate in unpollinated pistils. Pollination interrupted the flow of exudate, and a portion of the pistil secretion product was diverted toward the synthetic requirements of the developing pollen tubes. Two days after pollination, the specific radioactivity of pollen tube cytoplasm had reached a level comparable to canal exudate. Twenty-four hours later, the specific radioactivity of pollen tube wall substance was about 80% of the value found in canal exudate. Similar patterns of 14C incorporation and similar carbohydrate contents were obtained from self-and crosspollinated pistils, although the former contained pollen tubes of less than one-half the length of the latter.Evidence for a nutritional dependence of pollen tube wall formation on pistil secretion product has been obtained by injecting polysaccharide-rich, high molecular weight component, G-100-I, of stigmatic exudate from Lilium longiflorum into the stylar canal of freshly detached lily pistils and then crosspollinating the injected pistils (5). About one-fourth of the tube wall carbohydrate was traced to oligomeric and monomeric residues of injected G-100-I. In that study, many fac-

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“…When myoinositol-U-14C or -2-3H was given to detached pistils of L. Iongiflorwn, a portion of the label accumulated in stigmatic exudate (14). When these pistils were self-or cross-pollinated, the pollen tubes that developed and penetrated the labeled exudate were found to contain radioactive pectin (15). More recently, Kroh et al (12) have succeeded in demonstrating direct utilization of carbohydrate components of stigmatic exudate for pollen-tube wall formation.…”

mentioning

confidence: 95%

The Composition of Stigmatic Exudate from Lilium longiflorum

Labarca¹,

Kroh²,

Loewus³

1970

Plant Physiol.

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Stigmatic exudate, a secretion product recovered from the upper surface of Lilium longiflorum pistils, has been examined. Over 99% of the exudate is accounted for as water, carbohydrate, and protein. Exclusive of water, 95% is a high molecular weight, protein-containing polysaccharide composed of galactose, arabinose, rhamnose, glucuronic acid, and galacturonic acid.Detached pistils supplied with myo-inositol-U-14C, myoinositol-2-3H, D-glucose-1-14C, or L-proline-U-14C produce labeled stigmatic exudate. When myo-inositol is supplied, the exudate is rich in labeled arabinose and uronic acids, but some label also recycles through the hexose phosphate pool of secreting cells, causing label to appear in galactose and rhamnose residues. When glucose is provided, galactose is the major constituent labeled but all of the other carbohydrate constituents are also labeled. Proline produces a pattern very similar to that obtained with glucose.

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Incorporation of Label into Pollen Tube Walls from Myoinositol-labeled Lilium longiflorum Pistils

Cited by 72 publications

References 2 publications

Changes in the Amount and Composition of Stylar Canal Exudate after Self- or Cross-pollination in Self-incompatible Lilium longiflorum Thunb.

Changes in the Amount and Composition of Stylar Canal Exudate after Self- or Cross-pollination in Self-incompatible Lilium longiflorum Thunb.

The Nutritional Role of Pistil Exudate in Pollen Tube Wall Formation in Lilium longiflorum

The Composition of Stigmatic Exudate from Lilium longiflorum

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