Incorporation of maleic hydrazide into ribonucleic acid of Saccharomyces cerevisiae

Abstract

Food Chem. 1981, 29, 986-989 Johnson, A. W.; Rosebery, G.; Parker. C. Weed Res. 1976. 16. Cassady, J. M.; Howie, G. A. J. Chem. SOC., Chem. Commun. 1974, Cook, C. E.; Whichard, L. P.; Turner, B.; Wall, M. E. Science (Washington, D.C.) 1966, 154, 1189-1190. Cook, C. E.; Whichard, L. P.; Wall, M. E.; Egley, G. H.; Coggon, P.; Luhan, P. A.; McPhail, A. T. J. Am. Chem. SOC. 1972,94, 6198-6199.Maleic hydrazide [MH; 6-hydroxy-3(2H)-pyridazinone] was incubated with Saccharomyces cerevisiae and was found to be incorporated into the RNA of the cells where it substituted for cytosine rather than for uracil, its structural isomer. Incorporation was found to take place in the undifferentiated RNA fraction, as well as in tRNA, and may explain the mode of action of MH with regard to its ability to interfere with protein biosynthesis and cell growth.

Effect of maleic hydrazide on respiratory parameters of stored onion bulbs (Allium cepa L.)

“…A dilution of 9.10 -9 mol.L -1 inhibits the growth of a vast number of plants including Alliums. The effect of MH is to inhibit mitosis in the meristematic region, and plant metabolic studies have shown that, besides its many physiological side activities, MH acts as an antagonist of pyrimidine bases (Appleton et al, 1981). These authors noted that MH is incorporated into RNA of cells where it substituted for cytosine rather than for uracil, its structural isomer.…”

Determination of maleic hydrazide residues in garlic bulbs by HPLC

“…The biological effects of MH on plant growth and flowering were first described by Schoene and Hoffman [5]. MH acts as an antagonist of pyrimidine bases [6] by suppressing meristematic activity in the shoot apex thus reducing the associated deterioration in onion bulbs [7]. Following further absorption and translocation, the MH that becomes fixed within the plant is not completely metabolized but converted into ␤-d-glucoside as its predominant soluble metabolite [8].…”

Plant metabolic studies of the growth regulator maleic hydrazide

“…Maleic hydrazide (MH) is a plant growth regulator and herbicide applied mainly to tobacco, potatoes, and onions. Besides its many physiological side activities, it acts as an antagonist of pyrimidine bases (Appleton et al, 1981; Weed Science Society of America, 1989).…”