Incorporation of nanocapsules into gellan gum films: A strategy to improve the stability and prolong the cutaneous release of silibinin

Gehrcke, Mailine; Brum, Taíne de Bastos; Rosa, Lucas Saldanha da; Ilha, Bruna Dias; Soares, Fábio Zovico Maxnuck; Cruz, Letícia

doi:10.1016/j.msec.2020.111624

Cited by 26 publications

(32 citation statements)

References 38 publications

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“…It has been reported that a nanogel composed of hyaluronic acid and β-glucan efficiently activates the dendritic cells when incorporating an immunomodulator such as ovalbumin (OVA) [ 99 ]. Additionally, silibinin, a flavonoid that presents antioxidant and anti-inflammatory actions, has been delivered into the dermis using nanocapsules supported in a polymeric matrix to treat dermatitis [ 79 ]. Furthermore, nanoparticles of silica functionalized with PDMA (poly(2-(dimethylamine) ethyl methacrylate) were used to scavenge the cellular free DNA present in psoriasis, improving the symptoms in a murine model [ 100 ].…”

Section: Dermis As a Target For Nanotechnology-based Treatmentsmentioning

confidence: 99%

The Human Dermis as a Target of Nanoparticles for Treating Skin Conditions

et al. 2022

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Skin has a preventive role against any damage raised by harmful microorganisms and physical and chemical assaults from the external environment that could affect the body’s internal organs. Dermis represents the main section of the skin, and its contribution to skin physiology is critical due to its diverse cellularity, vasculature, and release of molecular mediators involved in the extracellular matrix maintenance and modulation of the immune response. Skin structure and complexity limit the transport of substances, promoting the study of different types of nanoparticles that penetrate the skin layers under different mechanisms intended for skin illness treatments and dermo-cosmetic applications. In this work, we present a detailed morphological description of the dermis in terms of its structures and resident cells. Furthermore, we analyze the role of the dermis in regulating skin homeostasis and its alterations in pathophysiological conditions, highlighting its potential as a therapeutic target. Additionally, we describe the use of nanoparticles for skin illness treatments focused on dermis release and promote the use of metal-organic frameworks (MOFs) as an integrative strategy for skin treatments.

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Section: Dermis As a Target For Nanotechnology-based Treatmentsmentioning

confidence: 99%

The Human Dermis as a Target of Nanoparticles for Treating Skin Conditions

et al. 2022

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“…Commonly, topical films are characterised in terms of their morphology and mechanical properties. For example, surface texture (smooth or rough) of the film can be observed using light microscopy, scanning electron microscopy (SEM) [15][16][17][18] and atomic force microscopy (AFM) [19][20][21]. Analytical control to confirm the concentration of ingredients in films can be performed with gold standard techniques such as highperformance liquid chromatography (HPLC) [17].…”

Section: Introductionmentioning

confidence: 99%

“…For example, surface texture (smooth or rough) of the film can be observed using light microscopy, scanning electron microscopy (SEM) [15][16][17][18] and atomic force microscopy (AFM) [19][20][21]. Analytical control to confirm the concentration of ingredients in films can be performed with gold standard techniques such as highperformance liquid chromatography (HPLC) [17]. However, to determine the content of an AI, fastidious extraction protocols need to be applied prior to analysis.…”

Section: Introductionmentioning

confidence: 99%

Confocal Raman Spectroscopic Imaging for Evaluation of Distribution of Nano-Formulated Hydrophobic Active Cosmetic Ingredients in Hydrophilic Films

et al. 2021

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Film-forming systems are highly relevant to the topical administration of active ingredients (AI) to the body. Enhanced contact with the skin can increase the efficacy of delivery and penetration during prolonged exposure. However, after the evaporation of volatile solvents to form a thin film, the distribution of the ingredient should remain homogenous in order to ensure the effectiveness of the formula. This is especially critical for the use of hydrophobic molecules that have poor solubility in hydrophilic films. In order to address this concern, hydroxyphenethyl esters (PHE) of Punica granatum seed oil were prepared as a nanosuspension stabilised by poloxamers (NanoPHE). NanoPHE was then added to a formulation containing polyvinyl alcohol (PVA) as a film forming agent, Glycerol as a plasticiser and an antimicrobial agent, SepicideTM HB. Despite their reliability, reference methods such as high-performance liquid chromatography are increasingly challenged due to the need for consumables and solvents, which is contrary to current concerns about green industry in the cosmetics field. Moreover, such methods fail to provide spatially resolved chemical information. In order to investigate the distribution of ingredients in the dried film, Confocal Raman imaging (CRI) coupled to Non-negatively Constrained Least Squares (NCLS) analysis was used. The reconstructed heat maps from a range of films containing systematically varying PHE concentrations highlighted the changes in spectral contribution from each of the ingredients. First, using NCLS scores it was demonstrated that the distributions of PVA, Glycerol, SepicideTM HB and PHE were homogenous, with respective relative standard deviations (RSD) of 3.33%, 2.48%, 2.72% and 6.27%. Second, the respective relationships between ingredient concentrations in the films and their Raman responses, and the spectral abundance were established. Finally, a model for absolute quantification for PHE was be constructed using the percentage of spectral abundance. The prepared %w/w concentrations regressed against predicted %w/w concentrations, displaying high correlation (R2 = 0.995), while the Root Mean Squared Error (0.0869% w/w PHE) confirmed the precision of the analysis. The mean percent relative error of 3.75% indicates the accuracy to which the concentration in dried films could be determined, further supporting the suitability of CRI for analysis of composite solid film matrix. Ultimately, it was demonstrated that nanoformulation of hydrophobic PHE provides homogenous distribution in PVA based film-forming systems independent of the concentration of NanoPHE used in the formula.

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“…The main advantages of these colloidal suspensions are improvement in the protection of the loaded active ingredient against physicochemical degradation as well as controlled release of drugs for a homogenous release [ 13 , 14 ]. Nanocapsules are specific polymeric nanoparticles with a core-shell organization in which the polymeric wall surrounds an oily core [ 15 ]. These nanocapsules have important advantages, such as higher drug loading capacity and improved ability to protect the encapsulated drug from degradation [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nanocapsules are specific polymeric nanoparticles with a core-shell organization in which the polymeric wall surrounds an oily core [ 15 ]. These nanocapsules have important advantages, such as higher drug loading capacity and improved ability to protect the encapsulated drug from degradation [ 15 , 16 ]. Moreover, they exhibit slow and sustained release of incorporated drugs which makes them suitable for cutaneous applications [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Design and Optimization of Cationic Nanocapsules for Topical Delivery of Tretinoin: Application of the Box-Behnken Design, In Vitro Evaluation, and Ex Vivo Skin Deposition Study

Ebrahimi

Mahjub

Haddadi

et al. 2021

BioMed Research International

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Cationic nanocapsules represent a promising approach for topical delivery purposes. We elaborated on a novel formulation based on the cationic nanocapsules to enhance the pharmacodynamic efficacy, user compliance, and photostability of tretinoin (TTN). To achieve this goal, TTN nanocapsules were prepared by the nanoprecipitation method. In order to statistically optimize formulation variables, a Box-Behnken design, using Design-Expert software, was employed. Three independent variables were evaluated: total weight of the cationic acrylic polymer ( X 1 ), oil volume ( X 2 ), and TTN amount ( X 3 ). The particle size and encapsulation efficiency percent (EE%) were selected as dependent variables. The optimal formulation demonstrated spherical morphology under scanning electron microscopy (SEM), optimum particle size of 116.3 nm, and high EE% of 83.2%. TTN-loaded nanocapsules improved photostability compared to its methanolic solution. The in vitro release study data showed that tretinoin was released in a sustained manner compared to the free drug. The ex vivo skin permeation study demonstrated that greater drug deposition into the epidermal region rather than the deep skin was observed with a gel containing TTN-loaded nanocapsules than that of drug solution, respectively. The skin irritation test revealed that the nanoencapsulation of the drug decreased its irritancy compared to the free drug. These results revealed the promising potential of cationic nanocapsules for topical delivery of tretinoin

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Incorporation of nanocapsules into gellan gum films: A strategy to improve the stability and prolong the cutaneous release of silibinin

Cited by 26 publications

References 38 publications

The Human Dermis as a Target of Nanoparticles for Treating Skin Conditions

The Human Dermis as a Target of Nanoparticles for Treating Skin Conditions

Confocal Raman Spectroscopic Imaging for Evaluation of Distribution of Nano-Formulated Hydrophobic Active Cosmetic Ingredients in Hydrophilic Films

Design and Optimization of Cationic Nanocapsules for Topical Delivery of Tretinoin: Application of the Box-Behnken Design, In Vitro Evaluation, and Ex Vivo Skin Deposition Study

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