Incorporation of new neurons in the olfactory bulb after paced mating in the female rat

Alvarado-Martínez, Reynaldo; Paredes, Raúl G.

doi:10.1016/j.bbr.2018.02.006

Cited by 9 publications

(14 citation statements)

References 35 publications

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“…with previous studies demonstrating that in female rats the first mating experience in pacing conditions increases the percentage of new cells that survive and differentiate into neurons in the . Additionally, when the number of paced mating sessions increased, the female rats that mated 10 times (once per week) showed an increase in the percentage of new neurons in the MOB but not in AOB (Alvarado- Martinez and Paredes, 2018). Thus, sexual activity during the first 16 days, a critical period for cell survival enhances the neuronal phenotype in the AOB but continuous sexual stimulation after this critical time appears to inhibit it.…”

Section: Discussionmentioning

confidence: 95%

“…In ungulates exposure to males for 48 h increases the number of new cells that proliferate in the dentate gyrus of the hippocampus (Hawken et al, 2009). However exposure to a male once for 1 h does not induce migration or survival into the olfactory bulbs in female rats (Arzate et al, 2013;Corona et al, 2016;Alvarado-Martinez and Paredes, 2018). In female mice chronic exposure to male pheromones (up to 21 days) increases cell survival (Larsen et al, 2008;Nunez-Parra et al, 2011;Oboti et al, 2011;Wu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…The first sexual experience in paced and non-paced conditions in female rats increases the proliferation of new cells in the RMS but does not increase the percentage of immature neurons . Paced mating increases the number of new cells that migrate to the Gr layer of the AOB but does not modify the number of new cells that survive in the MOB or AOB Alvarado-Martinez and Paredes, 2018). However, the first paced mating experience increases the percentage of new cells that survive and differentiate into mature neurons in the Gr layer of the AOB .…”

Section: Introductionmentioning

confidence: 90%

“…Brains were sliced in the sagittal plane at 30 µm and processed to detect BrdU positive cells (BrdU+ , Figures 2A,B). Immunohistochemistry was performed as previously reported (Arzate et al, 2011;Corona et al, 2011Corona et al, , 2016Unda et al, 2016;Alvarado-Martinez and Paredes, 2018). Brain sections were incubated in HCL 2N at 37 • C washed in Tris Buffer (TBS; Tris hydrochloride, Tris Base and Sodium chloride) and incubated with anti-BrdU mouse antibody (BD Bioscience; 1:2000) for 16 h at 4 • C. Later, brain sections were incubated in biotinylated anti-mouse IgG (1:500; Vector BA-2000) followed by incubation with the avidin-biotin complex (ABC, Vectastain).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…To determine if the new cells differentiate into mature neurons, brain slices were incubated in anti BrdU antibody and neuronal nuclei (NeuN) antibody to identify mature neurons. The procedure has been previously reported (Arzate et al, 2013;Corona et al, 2016;de Los Angeles et al, 2016;Unda et al, 2016;Alvarado-Martinez and Paredes, 2018). Briefly, brain sections were incubated with rat anti-BrdU (BD Bioscience, 1:800) and mouse anti-NeuN (1:250, Invitrogen) antibodies for 16 h at 4 • C. Later brain slices were incubated in rat anti-IgG Alexa Fluor 488 (1:1100, Invitrogen) and mouse Anti-IgG Alexa Fluor 568 (1:1100, Invitrogen).…”

Section: Immunofluorescencementioning

confidence: 99%

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Repeated Paced Mating Increases the Survival of New Neurons in the Accessory Olfactory Bulb

2020

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In female rats, the first sexual experience under paced mating conditions increases the number of newborn cells that migrate into the granular layer of the accessory olfactory bulb (AOB). Repeated paced mating has a potentiating effect on the number of new neurons that migrate to the AOB compared with a single session 15 days after paced mating. On the other hand, one paced mating session does no increases the survival of new cells 45 days after mating. In the present study, we evaluated if four paced mating sessions could increase the survival of new neurons in the AOB and main olfactory bulb (MOB) 45 days after females mated. Sexually naive female rats were ovariectomized, hormonally supplemented and randomly assigned to one of five groups: (1) Control, no sexual contact (C); (2) Four sessions in which females were exposed, without mating, to a sexually experience male rat (SE); (3) One session of paced mating (PM1); (4) Four sessions of paced mating (PM4); and (5) Four sessions of non-paced mating (NPM4). In the first behavioral test, females received the DNA synthesis marker 5-bromo-2 deoxyuridine and were euthanized 45 days later. Our data showed that the number of new cells that survived in the mitral cell layer of the AOB decreased when females were exposed to a sexually active male, in comparison to females that mated once pacing the sexual interaction. Repeated sexual behavior in pacing conditions did not increase the survival of new cells in other layers of the MOB and AOB. However, a significant increase in the percentage of new neurons in the granular and glomerular layers of the AOB and granular layer of the MOB was observed in females that mated in four sessions pacing the sexual interaction. In the group that paced the sexual interaction for one session, a significant increase in the percentage of neurons was observed in the glomerular layer of the AOB. Our data suggest that repeated paced mating increases the percentage of new neurons that survive in the olfactory bulb of female rats.

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Section: Discussionmentioning

confidence: 95%