Incorporation of reporter genes into mitochondrial DNA in budding yeast

Rzepka, Magdalena; Suhm, Tamara; Ott, Martin

doi:10.1016/j.xpro.2022.101359

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2023

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“…It would be interesting to have other reporters available and widely used. More recently, the laboratory of Martin Ott developed a system to incorporate reporter genes into S. cerevisiae mtDNA 70 that hopefully could improve and speed up the process of transformation.…”

Section: Challenges and Perspectivesmentioning

confidence: 99%

Biolistic transformation of the yeast Saccharomyces cerevisiae mitochondrial DNA

Franco

Barros

2023

IUBMB Life

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The insertion of genes into mitochondria by biolistic transformation is currently only possible in the yeast Saccharomyces cerevisiae and the algae Chlamydomonas reinhardtii. The fact that S. cerevisiae mitochondria can exist with partial (ρ− mutants) or complete deletions (ρ0 mutants) of mitochondrial DNA (mtDNA), without requiring a specific origin of replication, enables the propagation of exogenous sequences. Additionally, mtDNA in this organism undergoes efficient homologous recombination, making it well‐suited for genetic manipulation. In this review, we present a summarized historical overview of the development of biolistic transformation and discuss iconic applications of the technique. We also provide a detailed example on how to obtain transformants with recombined foreign DNA in their mitochondrial genome.

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Section: Challenges and Perspectivesmentioning

confidence: 99%