Incorporation of the bacterial reaction centre into dendrimersomes

Giustini, Mauro; Bellinazzo, Cristina; Galantini, Luciano; Mallardi, Antonia; Palazzo, Gerardo; Sennato, Simona; Bordi, F.

doi:10.1016/j.colsurfa.2012.01.040

Cited by 12 publications

(17 citation statements)

References 29 publications

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“…This morphology has been previously reported in dried liposomes and it has been attributed to a structural collapse due to the evaporation of water from vesicle interior and the adsorption on the substrate [ 20 , 21 ]. This behavior was also observed in dendrimersomes, as reported by Giustini et al [ 22 ]. Considering the apparent height of the higher outline of the structures (4.30 ± 0.91 nm) that compares with the typical dendrimersome bilayer thickness (5 to 8 nm) [ 2 , 22 , 23 ], it can be deduced that this value corresponds to the membrane thickness of the assemblies.…”

Section: Discussionsupporting

confidence: 83%

“…This behavior was also observed in dendrimersomes, as reported by Giustini et al [ 22 ]. Considering the apparent height of the higher outline of the structures (4.30 ± 0.91 nm) that compares with the typical dendrimersome bilayer thickness (5 to 8 nm) [ 2 , 22 , 23 ], it can be deduced that this value corresponds to the membrane thickness of the assemblies. The collapsed structure of the assemblies was confirmed by the AFM phase images, where a phase shift was observed.…”

Section: Discussionsupporting

confidence: 83%

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Self-Assembly Behavior of Amphiphilic Janus Dendrimers in Water: A Combined Experimental and Coarse-Grained Molecular Dynamics Simulation Approach

et al. 2018

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Amphiphilic Janus dendrimers (JDs) are repetitively branched molecules with hydrophilic and hydrophobic components that self-assemble in water to form a variety of morphologies, including vesicles analogous to liposomes with potential pharmaceutical and medical application. To date, the self-assembly of JDs has not been fully investigated thus it is important to gain insight into its mechanism and dependence on JDs’ molecular structure. In this study, the aggregation behavior in water of a second-generation bis-MPA JD was evaluated using experimental and computational methods. Dispersions of JDs in water were carried out using the thin-film hydration and ethanol injection methods. Resulting assemblies were characterized by dynamic light scattering, confocal microscopy, and atomic force microscopy. Furthermore, a coarse-grained molecular dynamics (CG-MD) simulation was performed to study the mechanism of JDs aggregation. The obtaining of assemblies in water with no interdigitated bilayers was confirmed by the experimental characterization and CG-MD simulation. Assemblies with dendrimersome characteristics were obtained using the ethanol injection method. The results of this study establish a relationship between the molecular structure of the JD and the properties of its aggregates in water. Thus, our findings could be relevant for the design of novel JDs with tailored assemblies suitable for drug delivery systems.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 83%

Self-Assembly Behavior of Amphiphilic Janus Dendrimers in Water: A Combined Experimental and Coarse-Grained Molecular Dynamics Simulation Approach

et al. 2018

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“…mechanical resistance, wall thickness, chemical groups exposed on the outer surface) has driven the search for new categories of amphiphiles other than phospholipids. [10][11][12] Two examples are represented by polymersomes [13][14][15] and dendrimersomes, [16][17][18][19][20][21][22] which are obtained through the self-assembly of amphiphilic block co-polymers or JDs, respectively. Whereas the potential of polymersomes in biomedical studies has been already demonstrated, 14,15 dendrimersomes have been proposed only very recently, and therefore their behaviour in biological environments is largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

Novel stable dendrimersome formulation for safe bioimaging applications

et al. 2015

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a Dendrimersomes are nanosized vesicles constituted by amphiphilic Janus dendrimers (JDs), which have been recently proposed as innovative nanocarriers for biomedical applications. Recently, we have demonstrated that dendrimersomes self-assembled from (3,5)12G1-PE-BMPA-G2-(OH) 8 dendrimers can be successfully loaded with hydrophilic and amphiphilic imaging contrast agents. Here, we present two newly synthesized low generation isomeric JDs: JDG0G1(3,5) and JDG0G1(3,4). Though less branched than the above-cited dendrimers, they retain the ability to form self-assembled, almost monodisperse vesicular nanoparticles. This contribution reports on the characterization of such nanovesicles loaded with the clinically approved MRI probe Gadoteridol and the comparison with the related nanoparticles assembled from more branched dendrimers. Special emphasis was given to the in vitro stability test of the systems in biologically relevant media, complemented by preliminary in vivo data about blood circulation lifetime collected from healthy mice. The results point to very promising safety and stability profiles of the nanovesicles, in particular for those made of JDG0G1(3,5), whose spontaneous self-organization in water gives rise to a homogeneous suspension. Importantly, the blood lifetimes of these systems are comparable to those of standard liposomes. By virtue of the reported results, the herein presented nanovesicles augur well for future use in a variety of biomedical applications.

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“…Lower PDI values support monodispersity of the formulations. 30,38 The size of the liposome depends on the lateral packing of lipid molecules within the membrane bilayer and is mainly governed by the van der Waals interactions between the hydrocarbon chains. 4,12 d h values for DHP + DPPC liposome, higher than other liposomes (DMPG + DPPC, DPP + DPPC, and DPPEth + DPPC), are due to the fact that the fraction of the head group region of higher density decreases with increasing hydrocarbon-chain length.…”

Section: Results and Discussionmentioning

confidence: 99%

Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer–Liposome Aggregates

et al. 2018

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Interaction between negatively charged liposomes and cationic polyamidoamine dendrimers of different generations was investigated through size, zeta potential, turbidity, electron microscopy, atomic force microscopy, fluorescence spectroscopy, and calorimetric studies. Liposomes with the binary combination of 1,2-dipalmitoyl- sn -glycero-3-phosphatidylcholine (DPPC) + dihexadecyl phosphate, DPPC + 1,2-dimyristoyl- sn -glycero-3-phosphoglycerol, DPPC + 1,2-dipalmitoyl- sn -glycero-3-phosphate, and DPPC + 1,2-dipalmitoyl- sn -glycero-3-phosphoethanol were stable up to 60 days. The electrostatic nature of dendrimer–lipid bilayer interaction was evidenced through charge neutralization and subsequent reversal upon added dendrimer to liposome. Dendrimer–liposome interaction depended on its generation (5 > 4 > 3) in addition to the charge, head groups, and hydrocarbon chain length of lipids. Fluorescence anisotropy and differential scanning calorimetry studies suggest the fluidization of the bilayer, although the surface rigidity was enhanced by the added dendrimers. Thermodynamic parameters of the interaction processes were evaluated by isothermal titration and differential scanning calorimetric studies. The binding processes were exothermic in nature. The enthalpy of transition of the chain melting of lipids decreased systematically with increasing dendrimer concentration and generation. Dendrimer–liposome aggregates were nontoxic to healthy human blood cell, suggesting the potential of such aggregates as drug delivery systems.

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Incorporation of the bacterial reaction centre into dendrimersomes

Cited by 12 publications

References 29 publications

Self-Assembly Behavior of Amphiphilic Janus Dendrimers in Water: A Combined Experimental and Coarse-Grained Molecular Dynamics Simulation Approach

Self-Assembly Behavior of Amphiphilic Janus Dendrimers in Water: A Combined Experimental and Coarse-Grained Molecular Dynamics Simulation Approach

Novel stable dendrimersome formulation for safe bioimaging applications

Biophysical Correlates on the Composition, Functionality, and Structure of Dendrimer–Liposome Aggregates

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