Incorporation of triclosan and acridine orange into liposomes for evaluating the susceptibility of Candida albicans

Romio, Karla B.; Santos, Kevin F. dos; Silva, Romário J. da; Pedro, Maria F. Costa; Kalck, Alessandro S.; Sousa, Marcos da Silva; Possamai, Leandro M.; Souto, Paula C. S.; Silva, Josmary R.; Souza, Nara C. de

doi:10.1016/j.jphotobiol.2017.06.034

Cited by 8 publications

(3 citation statements)

References 27 publications

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“…The obtained IC 50 value was 0.4 µM. These results are in agreement with the AO spectrum, which exhibits characteristic peaks in the UV region around 230 nm, 270 nm and 290 nm [ 50 ]; in the visible region at 490 nm (maximum absorbance) due to the AO molecule (monomer); and a shoulder at 470 nm that can turn into a peak with an increase in concentration, possibly due to AO dimers [ 51 ]. Like AO, several molecules with intense absorption peaks in the blue wavelength region (Soret peak) are described in the literature as very efficient PS [ 7 ].…”

Section: Resultssupporting

confidence: 77%

Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy: In Vitro Screening

Pivetta

Vieira

Silva

et al. 2023

IJMS

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Photodynamic therapy is a minimally invasive procedure used in the treatment of several diseases, including some types of cancer. It is based on photosensitizer molecules, which, in the presence of oxygen and light, lead to the formation of reactive oxygen species (ROS) and consequent cell death. The selection of the photosensitizer molecule is important for the therapy efficiency; therefore, many molecules such as dyes, natural products and metallic complexes have been investigated regarding their photosensitizing potential. In this work, the phototoxic potential of the DNA-intercalating molecules—the dyes methylene blue (MB), acridine orange (AO) and gentian violet (GV); the natural products curcumin (CUR), quercetin (QT) and epigallocatechin gallate (EGCG); and the chelating compounds neocuproine (NEO), 1,10-phenanthroline (PHE) and 2,2′-bipyridyl (BIPY)—were analyzed. The cytotoxicity of these chemicals was tested in vitro in non-cancer keratinocytes (HaCaT) and squamous cell carcinoma (MET1) cell lines. A phototoxicity assay and the detection of intracellular ROS were performed in MET1 cells. Results revealed that the IC50 values of the dyes and curcumin in MET1 cells were lower than 30 µM, while the values for the natural products QT and EGCG and the chelating agents BIPY and PHE were higher than 100 µM. The IC50 of MB and AO was greatly affected by irradiation when submitted to 640 nm and 457 nm light sources, respectively. ROS detection was more evident for cells treated with AO at low concentrations. In studies with the melanoma cell line WM983b, cells were more resistant to MB and AO and presented slightly higher IC50 values, in line with the results of the phototoxicity assays. This study reveals that many molecules can act as photosensitizers, but the effect depends on the cell line and the concentration of the chemical. Finally, significant photosensitizing activity of acridine orange at low concentrations and moderate light doses was demonstrated.

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Section: Resultssupporting

confidence: 77%

Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy: In Vitro Screening

Pivetta

Vieira

Silva

et al. 2023

IJMS

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“…4b) associated with appropriate markers can be used to evaluate cell viability. The vital dye, acridine orange, enters cells, mixes with intact DNA, and fluoresces green; if damaged, the cells appear orange-red [24]. The images confirm the susceptibility results presented in Fig.…”

Section: Antifungal Susceptibilitysupporting

confidence: 86%

“…This work used a viability assay based on the exclusion of the methylene blue dye [18][19][20][21][22], which acts as an indicator of cell viability. The counting in the Neubauer chamber was done with the help of the optical microscope, which considered the cells that were stained blue to be dead and the transparent ones to be viable [23].For morphological analysis, films with the dispersion of fungi were prepared by the casting technique [24], which involves the spreading of the material on a substrate. The entire procedure was performed in a laminar flow hood.…”

Section: Methodsmentioning

confidence: 99%

Latex membranes with methylene blue dye for antimicrobial photodynamic therapy

Sousa

Faria

Ribeiro

et al. 2021

Photochem Photobiol Sci

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The search for new materials that can be applied in the treatment of injured human tissues has led to the development of new dressings. Membranes have potential as dressing materials because they can be fitted to and interact with the tissue surface. In this study, we analyze the morphological properties and wettability of latex membranes, along with the incorporation of the photosensitizer methylene blue, in the context of the utility of the membranes in curative applications involving photodynamic therapy (PDT). It was observed that deposition of the photosensitizer into latex membranes increased both the surface roughness and wettability. Antifungal testing indicated that antimicrobial PDT assisted by the latex membranes incorporating methylene blue effectively inactivated Candida albicans.

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Mathematical models and fractal analysis for the investigation of the photodynamic inactivation in phytopathogenic microorganisms

Kalck

Pedro

Santos

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Incorporation of triclosan and acridine orange into liposomes for evaluating the susceptibility of Candida albicans

Cited by 8 publications

References 27 publications

Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy: In Vitro Screening

Phototoxic Potential of Different DNA Intercalators for Skin Cancer Therapy: In Vitro Screening

Latex membranes with methylene blue dye for antimicrobial photodynamic therapy

Mathematical models and fractal analysis for the investigation of the photodynamic inactivation in phytopathogenic microorganisms

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