Increase and Redistribution of Cardiac Mast Cells in Auricular Thrombosis

Radaszkiewicz, Thaddäus; Klappacher, G; Glogar, Dietmar; Sperr, Wolfgang R.; Grossschmidt, Karl; Bankl, Hans; Lechner, Klaus; Valent, Peter

doi:10.1161/01.cir.91.2.275

Cited by 51 publications

(51 citation statements)

References 39 publications

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“…SCF is a key factor influencing the survival, maturation, migration, and function of mast cells (15,19,20). In this study, SCF was shown to both initiate NF-B activation and enhance activation induced by TNF-␣ and anti-IgE.…”

Section: Discussionmentioning

confidence: 77%

NF-κB and TNF-α: A Positive Autocrine Loop in Human Lung Mast Cells?

Coward

Okayama

Sagara

et al. 2002

The Journal of Immunology

123

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The generation of cytokines, particularly TNF-α, by mast cells is crucial for the initiation of the allergic response. A key transcription factor involved in the synthesis of TNF-α is NF-κB. Using a mAb specific for the activated form of NF-κB, immunocytochemistry, confocal microscopy, and gel shift assays have been used in conjunction to localize this transcription factor to human lung mast cells and to study its activation. Activation of mast cells with stem cell factor (10 ng/ml) and anti-IgE (1 μg/ml) induced maximal activation of NF-κB at 4 and 2 h, respectively. In contrast, with TNF-α (5 ng/ml) maximal activation occurred within 15 min. Parallel falls in IκB were demonstrated. Confocal microscopy demonstrated the localization of the activated form of NF-κB to the nuclei of activated mast cells. NF-κB activation was verified using a gel shift assay. A supershift assay showed mast cell NF-κB to be composed primarily of p50 with smaller amounts of p65. No interaction with Abs for Rel-A, c-Rel, Rel-B, and p52 was seen. Immunocytochemistry and ELISAs showed TNF-α to be stored within mast cells and released into the extracellular environment following activation. The possible participation of TNF-α generated by mast cells in NF-κB activation by anti-IgE was investigated using a blocking Ab for TNF-α. The blocking Ab reduced NF-κB activation by anti-IgE by >50%, suggesting that the release of preformed mast cell-associated TNF-α acts as a positive autocrine feedback signal to augment NF-κB activation and production of further cytokine, including GM-CSF and IL-8.

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Section: Discussionmentioning

confidence: 77%

NF-κB and TNF-α: A Positive Autocrine Loop in Human Lung Mast Cells?

Coward

Okayama

Sagara

et al. 2002

The Journal of Immunology

123

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“…Mast cells play a fundamental role in the pathophysiology of inflammatory diseases through the elaboration and release of a myriad of proinflammatory (13,41,45) and immunoregulatory molecules (5,17,64), and they express a wide spectrum of surface receptors for cytokines and chemokines (8,53). Mast cells are present in the human heart (2,46), around coronary arteries (15,29), and in atherosclerotic plaques (27,28). Human heart mast cells (HHMC) have been implicated in the pathophysiology of coronary artery diseases (9,29), eosinophil myocarditis (14,47), and dilated cardiomyopathy (48).…”

mentioning

confidence: 99%

“…Mast cells are widely distributed in all vascularized tissues, including the heart (2,18,46). The strategic location of these cells within and around blood vessels and their ability to release inflammatory mediators (18,40,41), cytokines (5,17,19), and chemokines (64) suggest that they might be implicated in bacterial infections.…”

mentioning

confidence: 99%

Bacterial Immunoglobulin Superantigen Proteins A and L Activate Human Heart Mast Cells by Interacting with Immunoglobulin E

et al. 2000

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“…ise kar di yak MH'nin at ri yal trombüs olu şu mu ile il gi li ola bi le ceğini de öne sür müş -ler dir. 30 Ay rı ca, kalp trans plan tas yo nu son ra sı ge li şen re jek si yon lar da, MH'nin "fib rob last growth fac tor (FGF)" sal gı la ma sı nın ne den ol du ğu pe ri mi yo si tik ve in ters tisi yel fib ro zi sin önem li rol oy na dı ğı, ya pı lan bi yop si ler le gös te ril miş tir. 31,32 Yük sek asi dik FGF salgı lan ma sı, kar di yak al log reft vas kü lo pa ti si nin ge liş -me siy le an lam lı ola rak iliş ki li bu lun muş tur.…”

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The Role of Mast Cells in Cardiac Diseases and Surgery: Invited Commentary

Akgül¹,

Ünal²,

Kervan³

2011

Turkiye Klinikleri J Med Sci

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DAVETLİ YORUMÖ ÖZ ZE ET T Mast hücreleri, allerjik inflamatuar yanıtta etkili hücreler olarak bilinmektedir. Mast hücrelerinin histamin, triptaz, TNF-α, bFGF ve TGF-β gibi fibroblast fenotipi karakterinde ekstrasellüler matriks sentezi yapan mediyatörler ile özellikle fibrotik süreçte rol aldığı çalışmalar tarafından desteklenmektedir. Mast hücreleri ve diğer mediyatörleri, insan kalbini de içeren çeşitli hastalıklardan sorumlu tutulmuştur. Dilate kardiyomiyopatili insan kalbinde ve hayvan deneylerinde oluşturulmuş hipertansiyon, kalp krizi ve aortokaval fistül ile mitral regürjitasyona sekonder volüm yüklenmesinde mast hücre sayısında artış olduğu rapor edilmiştir. Aterosklerozun gelişimi özellikle granülosit, B ve T lenfositleri, mast hücreleri, dendritik hücreler ve progenitör hücreler gibi çeşitli tipteki immün sistem ile ilişkilidir. Koroner kan damarları çevresinde ve içinde mast hücrelerinin varlığı; kardiyak mast hücrelerinin aktivasyonu ve vazoaktif mediyatörlerin salınımı yoluyla iskemik kalp hastalığının patofizyolojisine katkıda bulunabileceğine işaret etmektedir. Bu çalışmada, mast hücrelerin kardiyak hastalıklardaki rolü incelenmeye çalışılmıştır. MAST HÜC RE LE Rİ hr lich 1878 yılında ani lin bo ya sı ile me tak ro ma tik bo ya nan ve da ha ön ce plaz ma hüc re le ri ola rak ta nım lan mış olan hüc re le rin fa go si toz yap tık la rı nı ve si top laz ma la rın da ki be lir gin gra nül le rin fa go si toz so-

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Increase and Redistribution of Cardiac Mast Cells in Auricular Thrombosis

Abstract: This study provides evidence that AUTHR is associated with MC increase and redistribution and MGF overexpression. The role of redistributed MC and their mediators in the pathophysiology of atrial thrombosis requires further investigation.

Cited by 51 publications

References 39 publications

NF-κB and TNF-α: A Positive Autocrine Loop in Human Lung Mast Cells?

NF-κB and TNF-α: A Positive Autocrine Loop in Human Lung Mast Cells?

Bacterial Immunoglobulin Superantigen Proteins A and L Activate Human Heart Mast Cells by Interacting with Immunoglobulin E

The Role of Mast Cells in Cardiac Diseases and Surgery: Invited Commentary

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