Increase EGFR Mutations Detection Rate in Lung Adenocarcinoma by Real-Time PCR Screening Followed by Direct Sequencing

Er, Tze‐Kiong; Lin, Chin-Wen; Liu, Ta-Chih; Chen, Chih-Chieh; Wang, Li‐Hsuan; Hsieh, Li-Ling; Tsai, Wen‐Chan

doi:10.1097/pdm.0000000000000037

Cited by 7 publications

(6 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A recent study indicated the rate of EGFR mutations was 39.6% among the 10,687 Asia patients [ 20 ]. Our result suggested that most of the EGFR mutations were located at exons 19 and 21 (accounting for 44.8% and 41.4%, respectively), which was similar toprevious studies [ 21 – 22 ]. Our result showed the mutation rate of EGFR in female patients was 47.8%, which was significantly higher than that in male patients (29.4%).…”

Section: Discussionsupporting

confidence: 90%

Detection of EGFR and KRAS gene mutations using suspension liquid-based cytology specimens in metastatic lung adenocarcinoma

Zhao

Qiu

et al. 2017

Oncotarget

View full text Add to dashboard Cite

BackgroundThe detection of EGFR and KRAS mutations of metastatic lung adenocarcinoma using liquid-based cytology suspension routine specimens from fine-needle aspiration remains controversial.ResultsThe DNA of all specimens was extracted and real time PCR was performed successfully. The rate of EGFR and KARS mutations was 37.7% (58/154) and 5.8% (9/154), respectively. EGFR mutation rate was significantly higher in females than that in males (47.8% vs. 29.4%, P = 0.019). There were no significant differences among different age groups or different tumor sites. These results of EGFR and KRAS mutations using LBC specimens were consistant with the tissue samples. In 30 patients treated with tyrosine kinase inhibitors, complete response, partial response, stable disease and progress disease was observed in 2, 10, 13 and 5 patients, respectively.ConclusionsLiquid-based cytology specimen is reliable and can be an alternative source for the detection of EGFR and KRAS mutations.Methods154 fine-needle aspiration cytologic samples were obtained from patients with metastatic lung adenocarcinoma. The specimens included 21 cases of mediastinal lymph node 123 cases of neck nodules and 10 cases of subcutaneous nodules. After the diagnosis and count of tumor cells performed by cytopathologists, liquid-based cytology specimens with sufficient tumor cells were used for EGFR and KRAS testing using real-time PCR.

show abstract

Section: Discussionsupporting

confidence: 90%

Detection of EGFR and KRAS gene mutations using suspension liquid-based cytology specimens in metastatic lung adenocarcinoma

Zhao

Qiu

et al. 2017

Oncotarget

View full text Add to dashboard Cite

show abstract

“…Both previous studies and our experience suggested that a real-time PCR-based method would detect the mutations of interest with the same efficiency as direct sequencing27282930. Only 13.95% of the patients screened were found to be free from any mutations in the genes analyzed in the present study.…”

Section: Discussionmentioning

confidence: 46%

Phenotype-genotype correlation in multiple primary lung cancer patients in China

Yang

Jiang

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Due to recent advances in high-resolution detection technology, multiple primary lung cancer (MPLC) is becoming an increasingly common diagnosis. However, the genotype-phenotype correlations in MPLC patients have not yet been assessed. In this study, we analyzed the clinical and pathological data for 129 consecutive MPLC patients who received curative surgery at the Tongji University Shanghai Pulmonary Hospital, China. We have screened 129 patients in the present study and found mutations in EGFR, BRAF, ROS1 and KRAS genes, as well as the rearrangement of the EML4-ALK gene in 113 patients. The mean patient age was 59.9 (25–78) years old and 41 patients were males (31.8%). Among the total patients, 123 (95.4%) had two primary lesions, 5 (3.9%) had three primary lesions, and 1 (0.8%) had four primary lesions. In 38.8% of the patients, all lesions were located on only one side of the body. Most of the detected mutations (98 patients) were in the EGFR gene. The patients exhibited significant differences in the EGFR mutation, age at diagnosis, and foci location.

show abstract

“…Pušenje se smatra najznačajnijim faktorom rizika za pojavu ove bolesti kod muškaraca ali ne i kod žena. Bazična, translaciona i klinička istraživanja tokom poslednjih godina dovela su do značajnih otkrića koja su uticala na promenu medicinskih stavova počevši od patogeneze, preko klasifikacije i dijagnostike pa sve do terapije ove bolesti (8)(9)(10)(11).…”

Section: Karcinom Plućaunclassified

“…Najbolje ispitane su mutacije receptora za epidermni faktor rasta (EGFR -eng. epidermal growth factor receptor) koje se javljaju u adenokarcinomu pluća (9,10). Kod pripadnika bele rase ova mutaci-ja se javlja u oko 10%-15% slučajeva, a u azijskim populacijama u oko 30%.…”

Section: Karcinom Plućaunclassified

Genetic factors in the development and course of pulmonary diseases

Pešut¹,

Nagorni-Obradović²,

Jovanović³

et al. 2014

Medicinska istraživanja

View full text Add to dashboard Cite

The year 2013 denoted the 50th anniversary of the discovery of a1-antitrypsin deficiency. Apart from genetic changes in cystic fibrosis, discovered in 1989, the condition was the only recognized genetic disturbance in pulmonary diseases for years. In the meantime, many studies have been undertaken, especially in the diseases of unknown causes such as sarcoidosis, but also in those of well-known infectious cause like tuberculosis. Genetic studies in tuberculosis were especially COM-plex and directed both towards host susceptibility and genetics of Mycobacterium tuberculosis with special reference to the mechanisms of drug resistance development. Apart from animal studies at the beginning of the second half of the 20th century, twins and major histocompatibility system studies, population-genetic and pedigree analysis were performed, then, biomarkers such as ABO system blood types analysis and many others. Availability of molecular techniques allowed great step forward and brought new knowledge on the genetics of pulmonary diseases, including epigenetics. The purpose of the review is to present new findings and highlight importance of current opinion on genetic factors influence in the development, course and prognosis of the most prevalent chronic non-infectious and rare pulmonary diseases.

show abstract

Increase EGFR Mutations Detection Rate in Lung Adenocarcinoma by Real-Time PCR Screening Followed by Direct Sequencing

Cited by 7 publications

References 46 publications

Detection of EGFR and KRAS gene mutations using suspension liquid-based cytology specimens in metastatic lung adenocarcinoma

Detection of EGFR and KRAS gene mutations using suspension liquid-based cytology specimens in metastatic lung adenocarcinoma

Phenotype-genotype correlation in multiple primary lung cancer patients in China

Genetic factors in the development and course of pulmonary diseases

Contact Info

Product

Resources

About