Increase in apoptotic effect of Panax ginseng by microwave processing in human prostate cancer cells: in vitro and in vivo studies

Park, Jun Yeon; Choi, Pilsoon; Kim, Ho-kyong; Kang, Ki Sung; Ham, Jungyeob

doi:10.1016/j.jgr.2015.04.007

Cited by 45 publications

(37 citation statements)

References 29 publications

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“…Extracts from traditionally-cultivated Panax ginseng plants have been found to have anti-carcinogenic effects against various human cancer cell lines (stomach, cervical, colorectal, breast adenocarcinoma, lung carcinoma) (Lee et al , 2016 with IC 50 values ranging between 0.25 and 2.84 mg mL −1 depending on the tested cell line (Lee et al , 2016; similar values were obtained in the present study for our P. quinquefolium extracts. In contrast, Park et al (2016) reported P. ginseng root extract to have much stronger anticancer activity against prostate cancer cell lines (IC 50 0.05-0.075 mg mL −1 ).…”

Section: The Effects Of Fhr and Bhr On The Viability Of Glioblastomamentioning

confidence: 90%

The content of triterpene saponins and phenolic compounds in American ginseng hairy root extracts and their antioxidant and cytotoxic properties

Kochan

Szymańska

Wielanek

et al. 2019

Plant Cell Tiss Organ Cult

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Panax quinquefolium is a perennial herb of the Araliaceae family native to North America. Its roots have been used in traditional and Chinese medicine. The aim of this study was to determine the phenolic profile of methanolic extracts of P. quinquefolium hairy roots cultivated in flasks and a bioreactor, as well as extracts from the roots of three-year-old field-grown plants. Additionally, the phenol and ginsenoside components of the tested extracts were identified by HPLC, and their antioxidant and cytotoxic properties were evaluated. The antioxidant effect was evaluated by FRAP (ferric reducing antioxidant power), and ABTS ([2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation scavenging tests, and their effect on the viability of the glioblastoma cell (T98G) line was measured using the 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The LC-MS/MS analysis revealed the presence of 16 phenolic compounds identified as phenolic acids (ten compounds) or flavonoids (six compounds). The highest phenol content was observed in the transformed roots of flask-grown P. quinquefolium (1.6 mg g −1 d.w.), followed by these grown in the bioreactor (1.1 mg g −1 d.w.). However, the highest ginsenoside content was found in the roots of the naturally-cultivated plants (67.6 mg g −1 d.w.). The methanolic extracts from hairy root culture of P. quinquefolium appear to have significant antioxidant and cytotoxic potential. Such transformed American ginseng root cultures could represent a potential source of bioactive metabolites for the food or pharmaceutical industry. Key message First time phenolic compound level, antioxidant and cytotoxic properties were estimated in hairy root cultures of Panax quinquefolium cultivated in flasks and nutrient sprinkle bioreactor. Ginsenosides content was also determined.

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Section: The Effects Of Fhr and Bhr On The Viability Of Glioblastomamentioning

confidence: 90%

The content of triterpene saponins and phenolic compounds in American ginseng hairy root extracts and their antioxidant and cytotoxic properties

Kochan

Szymańska

Wielanek

et al. 2019

Plant Cell Tiss Organ Cult

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“…Cancer stem cells (CSCs) have been regarded as the root of tumor progression and drug resistance, however, there is no effective drug targeting CSCs yet. Ginsenoside is an important active ingredient of ginseng and it has been used as an anticancer Chinese medicine in the treatment of malignant tumors such as melanoma and prostate cancer . Ginsenoside Rg3, the main component of Ginsenoside, has been shown to inhibit tumor progression and chemoresistance, for example, Ginsenoside Rg3 attenuates temozolomide resistance and epithelial‐mesenchymal transition (EMT) progression in glioblastoma; Ginsenoside Rg3 inhibits prostate cancer cell proliferation through inducing cell cycle arrest .…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rg3 attenuates the osimertinib resistance by reducing the stemness of non‐small cell lung cancer cells

Tan

Lin

Zeng

et al. 2020

Environmental Toxicology

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In the present study, we found that Ginsenoside Rg3 attenuated the stemness of non-small cell lung cancer (NSCLC) cells, evident by decreasing spheroid formation ability, ALDH1 activity and stemness marker expression. Furthermore, osimertinibresistant NSCLC cells displayed a stronger stemness than the parental cells.Ginsenoside Rg3 reduced the stemness and osimertinib resistance of osimertinibresistant cells. RNA-sequencing revealed that Hippo signaling was shown on the top of the most upregulated pathways regulated by Ginsenoside Rg3 in NSCLC cells, and YAP/TAZ expression was suppressed by Ginsenoside Rg3. Notably, the inhibitor of Hippo signaling attenuated the effects of Ginsenoside Rg3 on the stemness of NSCLC cells. Therefore, Ginsenoside Rg3 attenuates the osimertinib resistance of NSCLC cells via suppressing the stemness, which is dependent on Hippo pathway. K E Y W O R D SGinsenoside Rg3, osimertinib, non-small cell lung cancer, Hippo

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“…Ginsenoside 20(R)-Rg3, but not 20(S)-Rg3, suppressed lung cancer migration, invasion, and anoikis resistance [18]. However, ginsenoside 20(S)-Rg3, but not 20(R)-Rg3, induced apoptotic cell death in human gastric cancer AGS cells by activating caspases [19], [20].…”

Section: Resultsmentioning

confidence: 95%

Protective effect of ginsenoside Rh3 against anticancer drug-induced apoptosis in LLC-PK1 kidney cells

Lee

Kang

2017

Journal of Ginseng Research

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BackgroundGinsenosides are active components of Panax ginseng that exert various health benefits including kidney protection effect. The medicinal activity of ginsenosides can be enhanced by modulating their stereospecificity by heat processing. Ginsenosides Rk2 and Rh3 represent positional isomers of the double bond at C-20(21) or C-20(22).MethodsThe present study investigated the kidney-protective effects of ginsenosides Rk2 and Rh3 against cisplatin, a platinum based anticancer drug, induced apoptotic damage in renal proximal LLC-PK1 cells.ResultsAs a result, ginsenoside Rh3 shows a stronger protective effect than that shown by Rk2. Cisplatin-induced elevated protein levels of phosphorylated c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), p38, and cleaved caspase-3 decreased after cotreatment with ginsenoside Rh3. The increase in the percentage of apoptotic LLC-PK1 cells induced by cisplatin treatment also significantly reduced after cotreatment with ginsenoside Rh3.ConclusionThese results demonstrate that inhibition of the JNK and ERK mitogen-activated protein kinase signaling cascade plays a critical role in mediating the renoprotective effect of ginsenoside Rh3.

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Increase in apoptotic effect of Panax ginseng by microwave processing in human prostate cancer cells: in vitro and in vivo studies

Cited by 45 publications

References 29 publications

The content of triterpene saponins and phenolic compounds in American ginseng hairy root extracts and their antioxidant and cytotoxic properties

The content of triterpene saponins and phenolic compounds in American ginseng hairy root extracts and their antioxidant and cytotoxic properties

Ginsenoside Rg3 attenuates the osimertinib resistance by reducing the stemness of non‐small cell lung cancer cells

Protective effect of ginsenoside Rh3 against anticancer drug-induced apoptosis in LLC-PK1 kidney cells

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