Increase in ploidy in yeasts as a response to stressing media

Talbot, Nicholas J.; Wayman, Morris

doi:10.1007/bf00165882

Cited by 11 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This indicates that ploidy plays a role in the handling of environmental stress by P. anomala . A connection between stress tolerance and ploidy has been observed in xylose fermenting yeasts, which raised their ploidy level after cultivation in wood hydrolysate [21]. We have currently no explanation on how ploidy influences stress tolerance of yeasts.…”

Section: Resultsmentioning

confidence: 97%

Influence of ethyl acetate production and ploidy on the anti-mould activity ofPichia anomala

Fredlund¹,

Druvefors²,

Olstorpe³

et al. 2004

FEMS Microbiology Letters

View full text Add to dashboard Cite

A diploid and a haploid strain of Pichia anomala were tested for their biocontrol ability against the spoilage mould Penicillium roqueforti in glass tubes filled with grain at two water activities (aw). At aw 0.98, the two yeast strains grew and inhibited mould growth equally well and showed similar patterns of ethyl acetate production, reaching maximum values of 10-14 microg ml(-1) headspace. At aw 0.95, both growth and biocontrol performance of the haploid strain were reduced. Ethyl acetate formation was also substantially reduced, with maximum headspace concentrations of 4 microg ml(-1). We conclude that ethyl acetate is a major component of the anti-mould activity. The inhibitory effect of ethyl acetate was confirmed in a bioassay where the pure compound reduced biomass production of P. roqueforti.

show abstract

Section: Resultsmentioning

confidence: 97%

Influence of ethyl acetate production and ploidy on the anti-mould activity ofPichia anomala

Fredlund¹,

Druvefors²,

Olstorpe³

et al. 2004

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…It is conceivable that stress might increase the frequency of polyploidization. For example, the ploidy of Candida shehatae and Pichia stipitis increases during growth on the poor medium of wood hydrolysates, presumably enabling improved efficiency in xylose fermentation (Talbot and Wayman, ). Similarly, treatment of C. albicans with fluconazole‐based drugs interferes with cytokinesis, thus triggering genome duplication, which in turn increases genome instability that might accelerate the appearance of an aneuploidy karyotype that confers resistance (Harrison et al ., ).…”

Section: Polyploidy Aneuploidy and Survival Of Stressmentioning

confidence: 99%

Ploidy changes and genome stability in yeast

Storchová

2014

Yeast

View full text Add to dashboard Cite

Eukaryotic organisms maintain karyotypes with constant chromosome number, but polyploid cells that contain more than two sets of chromosomes can frequently be found. On the one hand, polyploidization is likely to provide some beneficial effects, as naturally occurring polyploid cells can be readily found. On the other hand, polyploidization profoundly affects cell physiology, which may be detrimental to cells. Additionally, polyploidy leads often to aneuploidy and diversification of genetic information; therefore, it has always been considered a prominent driving force in evolution. Recently tetraploid-derived aneuploidy was suggested as a possible mechanism for resistance to fungicides. Another prominent example of the effects of tetraploid-derived aneuploidy is cancer, in which up to one-third of tumours likely originate through tetraploid intermediates. Studying the cellular consequences of polyploidization in human cells is challenging. In contrast, polyploid and aneuploid cells can be easily generated and analysed in the budding yeast Saccharomyces cerevisiae as well as in other yeast species. This, together with the naturally occurring yeast polyploids and aneuploids, provides a valuable model to study the effects of abnormal chromosome numbers on cellular physiology. Thus, the yeast model may provide novel insights into the general mechanisms of genomic instability in eukaryotes and improve our understanding of the consequences of ploidy changes and their relevance for disease.

show abstract

“…It is not known if these characters are a consequence of the strong selection imposed on these strains. Maintenance of aneuploidy or polyploidy could confer a greater protection against spontaneous lethal recessive mutations (Tavares et al, 1988) or could enhance dosage of important genes improving fermentation (Adams et al, 1992), such as the MAL genes (Mowshowitz, 1979;Stewart et al, 1981), the genes implicated in the xylose fermentation pathway (Talbot and Wayman, 1989), or in ethanol production, such as ADH1 (Dilorio et al, 1987). On the other hand, although it was presumed that polyploidy prevents evolutionary changes, Paquin and Adams (1983) provide direct evidence that the speed of evolution of diploid strains is greater than that of haploid strains.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Relative Ploidy in DifferentSaccharomyces cerevisiae Strains used for Fermentation and ‘Flor’ Film Ageing of Dry Sherry-type Wines

Guijo

Mauricio

Salmon

et al. 1997

Yeast

View full text Add to dashboard Cite

The full chromosomal karyotype of six enological Saccharomyces cerevisiae strains used for fermentation and biological ageing of sherry‐type wines was studied. A genetic method based on the analysis of segregation frequencies of auxotrophic markers, among random spore progeny of hybrids, constructed between laboratory and industrial wine strains (Bakalinsky and Snow, 1990) was used. This method was combined with the analysis of strains by pulsed‐field gel electrophoresis. The results obtained clearly indicate the presence of two, three or four copies of a chromosome in the industrial strains examined, and thus confirm that aneuploidy/polyploidy is not uncommon in these strains. In all strains examined, chromosome XIII polysomy is observed. This chromosome contains the ADH2 and ADH3 loci, that code for the ADHII and ADHIII isoenzymes of alcohol dehydrogenase, which are involved in ethanol oxidative utilization during biological ageing of wines. Tetrad analysis for the ‘flor formation’ character suggests two possibilities: this character is either regulated by at least a digenic system, or by only one gene present on a chromosome which is, at least, disomic.© 1997 John Wiley & Sons, Ltd.

show abstract

Increase in ploidy in yeasts as a response to stressing media

Cited by 11 publications

References 13 publications

Influence of ethyl acetate production and ploidy on the anti-mould activity ofPichia anomala

Influence of ethyl acetate production and ploidy on the anti-mould activity ofPichia anomala

Ploidy changes and genome stability in yeast

Determination of the Relative Ploidy in DifferentSaccharomyces cerevisiae Strains used for Fermentation and ‘Flor’ Film Ageing of Dry Sherry-type Wines

Contact Info

Product

Resources

About