Increase in RANKL: OPG Ratio in Synovia of Patients with Temporomandibular Joint Disorder

Wakita, Takeshi; Mogi, Makio; Kurita, K.; Kuzushima, Masatoshi; Togari, Akifumi

doi:10.1177/154405910608500709

Cited by 34 publications

(25 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, it has been shown previously that significant amounts of sRANKL (10.9 ± 2.2 pg/ml) are produced by OPG-knockout chondrocytes in vitro, indicating that sRANKL can indeed be produced by chondrocytes in the setting of an altered RANKL/OPG ratio [23]. In human osteoarthritis of the temporomandibular joint, sRANKL levels in the synovial fluid were not increased compared to normal [24]; however, supporting our findings sRANKL is increased in the serum of patients with primary knee OA [25]. Since elevated RANKL levels were mainly in the deep cartilage it might diffuse into the underlying bone and then into the local vasculature rather than through the layer of cartilage into the synovial fluid.…”

Section: Discussionmentioning

confidence: 92%

The expression of RANKL and OPG in the various grades of osteoarthritic cartilage

et al. 2011

View full text Add to dashboard Cite

The objective of the study was to determine whether cartilage expression of the bone regulating molecules receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) varies between the different grades of osteoarthritis (OA). Cartilage samples were obtained from 30 patients undergoing total hip/knee replacement surgery. Tissue sections were stained with Safranin O and graded. Immunohistochemical staining was then performed, and levels of RANKL and OPG expression were assessed using a semi-quantitative scoring system. In addition, levels of mRNA encoding for RANKL and OPG were determined by a relative real-time reverse transcription-polymerase chain reaction technique. We found that expression of RANKL protein, mRNA expression, and the ratio of RANKL: OPG mRNA was greater in grade 2 cartilage in comparison with grade 0 cartilage (P < 0.05). Increased RANKL staining in the grade 2 cartilage was predominantly in the peri-cellular region of the middle and deep zones as well as in the matrix of the superficial zone. OPG mRNA expression was greater in grade 3 cartilage in comparison with grade 0 cartilage (P < 0.05). Cartilage and subchondral bone are in close proximity and soluble proteins produced in the cartilage are likely to move from one compartment to the other. Our finding of increased expression of RANKL in grade 2 OA cartilage might explain the increase in bone turnover reported in the subchondral bone of OA patients. The changes seen in the different grades of tissue may also indicate that this effect occurs during the early stages of OA development.

show abstract

Section: Discussionmentioning

confidence: 92%

The expression of RANKL and OPG in the various grades of osteoarthritic cartilage

et al. 2011

View full text Add to dashboard Cite

show abstract

“…[6,8,10] Since OPG decrease ratios are not so high in the GCF of gingivitis patients in comparison with those in other rheumatoid and periodontal diseases, [6][7][8] this might explain why gingivitis is a relatively mild inflammative disease and not bonedestructive disease. Previous data taken together with our current findings suggest that inflammation has a common mechanism to induce an increase in the RANKL:OPG ratio in the body fluid.…”

Section: Discussionmentioning

confidence: 94%

“…EGF appears to contribute to inflammatory responses, as well as to other physiological and pathological processes. [5] Whereas a recent study also suggests the involvement of the receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in the pathogenesis of bone-destructive disease such as rheumatoid arthritis and periodontal disease, [6][7][8][9][10][11] no one has examined the level of RANKL and OPG in the body fluid of the patients with gingivitis except for one. [12] Several investigators have examined gingival crevicular fluid (GCF) for cellular immune-response indicators, as the levels of such indicators are considered to serve as possible markers of active periodontal disease, [13][14][15][16][17][18] but, thus far, no one has looked at the level of growth factors, especially EGF and TGF-a in gingivitis.…”

Section: Drop In Transforming Growth Factor-a and Osteoprotegerin Levmentioning

confidence: 97%

Drop in Transforming Growth Factor-α and Osteoprotegerin Level in Gingival Crevicular Fluid from Patients with Gingivitis

Otogoto

Mogi

2009

Journal of Immunoassay and Immunochemistry

View full text Add to dashboard Cite

Inflammatory mediators, especially cytokine, play a central role in the pathogenesis of gingivitis. The aim of this study was to identify and quantify the various growth factors, and cytokines in the gingival crevicular fluid (GCF) of patients with gingivitis, as compared with those of control subjects. The levels of cytokine in the samples were determined by their respective ELISAs. The transforming growth factor (TGF)-alpha and osteoprotegerin (OPG) level were significantly lower in patients with gingivitis than in the controls (p < 0.05). Also, there was a positive correlation between TGF-alpha and OPG levels (r = 0.761). These results suggest that the decrease in growth factor TGF-alpha is associated with the pathophysiology and/or the progress of gingivitis.

show abstract

“…[8] In addition to the increase in the level of RANKL protein in the inflamed synovium of rheumatoid arthritis patients, [9] we demonstrated earlier that OPG concentrations in the synovial fluid were lower in patients with rheumatoid arthritis (as compared with patients with other forms of arthritis), which resulted in an increased local and systemic RANKL:OPG ratio. [10] We also demonstrated an elevation of RANKL and a decrease in OPG in gingival crevicular fluid of patients with periodontal disease [11,12] and in synovia of patients with tempomandibular joint disorder, [13] suggesting that RANKL and OPG are important factors involved in bone and joint destruction in humans, and that OPG has a protective role in bone-destructive diseases.…”

Section: Introductionmentioning

confidence: 91%

Elisa for Rankl-Opg Complex in Mouse Sera

Mogi

Kondo

2010

Journal of Immunoassay and Immunochemistry

View full text Add to dashboard Cite

A sensitive sandwich enzyme-linked immunosorbent assay (ELISA) for the mouse RANKL-OPG complex was developed by utilizing a polyclonal antibody that recognizes mouse soluble RANKL as an immobilized capture component and mouse OPG IgG labeled with peroxidase. We could quantify the RANKL-OPG complex level (detection limit: 1 pmol/L). Employing this assay system, we demonstrated that the RANKL-OPG complex was constitutively present in the serum of OPG+/- mice, but not in that of OPG-/- or wild-type C57BL/6 J mice.

show abstract

Increase in RANKL: OPG Ratio in Synovia of Patients with Temporomandibular Joint Disorder

Cited by 34 publications

References 23 publications

The expression of RANKL and OPG in the various grades of osteoarthritic cartilage

The expression of RANKL and OPG in the various grades of osteoarthritic cartilage

Drop in Transforming Growth Factor-α and Osteoprotegerin Level in Gingival Crevicular Fluid from Patients with Gingivitis

Elisa for Rankl-Opg Complex in Mouse Sera

Contact Info

Product

Resources

About