INCREASE OF (Ca2+-Mg2+)-ADENOSINE TRIPHOSPHATASE ACTIVITY IN RAT TESTIS UNDERGOING REGRESSION OF THE GERMINAL EPITHELIUM

Delhumeau-Ongay, G.; Trejo-Bayona, R.; Alvarez-Buylla, R; Lara-Vivas, L.

doi:10.1530/jrf.0.0340149

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…Like other mammals, alkaline phosphatase, acid phosphatase and adenosine triphosphatase were mainly demonstrated in all the elements of germinal epithelium of seminiferous tubules and Sertoli cells of the testis of the musk shrew (Singh and Mathur -1968). Delhumeau-Ongay et al (1973) had noticed that increase in testicular ATPase activity is associated with the regression of germinal epithelium. A decrease in testicular ATPase activity with advancing age is also attributed to decrease in spermatogenic activity (Ikegwuonu and Aire -1977).…”

Section: Discussionmentioning

confidence: 97%

“…Specific enzymes have been suggested as possible markers for the study of physiology of particular cell types of the testis (Hodgen -1977). The association of acid phosphatase, alkaline phosphatase and adenosine triphophatase with the physiology of the seminiferous tubules were inferred from alteration in their activities after suppression of germ cells in various experimental and functional conditions (Males and Turkington -1971;Tice and Barnett -1963;Delhumeau-Ongay et al -1973;Guha and Vanha-Perttula -1980). It has been suggested that testosterone plays an important role in the maintenance and differentiation of germ cells (Steinberger and Steinberger -1974;Vernon et al -1975;Marshall et al -1986).…”

Section: Introductionmentioning

confidence: 99%

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Testosterone Propionate Induced Changes in Testicular Phosphatases of Musk Shrew (Suncus murinus L.)/Testosteronpropionatinduzierte Veränderungen der Hoden-Phosphatasen von Bisammaus (Suncus murinus L.)

Mohanty

Chainy

2009

Andrologia

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In order to investigate the role of testosterone (TP) on the physiology of the testis of the musk shrew, activities of acid phosphatase, alkaline phosphatase and adenosine triphosphatase, and DNA, protein and phospholipid contents ot the testis were compared under high and suppressed testosterone conditions. The result indicates that testosterone propionate administration to intact shrews resulted in a significant increase in the activities of testicular enzymes. Following TP the testis phospholipid: DNA ratio increased, whilst no change was observed in the enzyme activities in response to aminoglutethimide phosphate. The role of testosterone in regulating testis function of this non-scrota1 primitive eutherian mammal is discussed.Zusammenfassung: Um die Rolle von Testosteron in der Physiologie des Hodens der Bisammaus zu untersuchen, wurde die Aktivitat der sauren, der alkalischen Phosphatase und der ATP, sowie von DNA, Protein und Phospholipidbestandteilen des Hodens unter 500 ugTP/100 g KG und unter 4 mg Aminoglutethimid/lOO g KG studiert. Die Behandlung erfolgte uber vier Tage. Es ergab sich, daR TP zu einem signifikanten Anstieg der Hodenenzyme fuhrt, das Verhaltnis Hodenphospholipid: DNA stieg signifikant an. andrologia 21, No. 4 (1989)

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Testosterone Propionate Induced Changes in Testicular Phosphatases of Musk Shrew (Suncus murinus L.)/Testosteronpropionatinduzierte Veränderungen der Hoden-Phosphatasen von Bisammaus (Suncus murinus L.)

Mohanty

Chainy

2009

Andrologia

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“…The decrease in activity of acid phosphatase in the testes at days 40 and 70 can be supposed to be brought about with the depletion of cell population of the seminiferous tubules of the treated rats. Earlier studies have shown that the alteration in the activities of testicular phosphatase can be reflected with the suppression of functional changes in the testicular cell types [8,11,23,34].…”

Section: Discussionmentioning

confidence: 99%

Histophysiological Changes of the Testicular Tissue Due to Busulphan Administration in the Wild Indian House Rat (Rattus Rattus)

Aich¹,

Manna²

2001

Acta Biologica Hungarica

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The results of the present study indicate the antispermatogenic activity of Busulphan or Myleran (1,4-dimethane-sulphonoxy butane) on the testicular tissue of adult male Indian house rat, Rattus rattus. Single oral dose of Busulphan (10 mg/Kg body weight) was administered and its activity was noticed at 10, 40, 70 and 100 days of posttreated animals. Histological observation and quantitative histological study indicates no major alteration in the relative percentages of primary spermatocytes, spermatid and Sertoli cells at 10 days of posttreatment. But there was a gradual decrease in the seminiferous tubular diameter at 40 and 70 days of post treated groups. However, the Leydig and Sertoli cells morphology and number remained normal in all the treatment groups. At 40 days, the normal cellular associations in all the tubules were disrupted. The tubules constituted only spermatogonia, Sertoli cells and some zygotene spermatocytes. At 70 days, repopulation of Type A, Type B spermatogonia, resting and zygotene spermatocytes occurred at this stage. The tubules were still devoid of pachytene spermatocytes, spermatid and spermatozoa. At 100 days, active spermatogenesis was observed in majority of the tubules. The various types of germ cell population were regaining towards normalcy. Histochemical studies clearly revealed that due to busulphan administration there was no major alteration in the intensities of some key enzymes (i.e. delta5 3beta-HSDH and 17beta-HSDH) involved in the biosynthesis of steroid hormones. Only the acid phosphatase activity was slightly depressed within the 40th and 70th days of posttreatment. Sudanophilic lipid materials increased in the interstitium of all the busulphan post treated groups. The changes which were noticed due to busulphan treatment regained normalcy at 100 days of post treated animals. The mode of action of Busulphan on the testicular tissue of adult Indian house rat (Rattus rattus) has been pointed out and discussed.

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Light and electron microscopic localization of ATPase in normal and degenerating testes of syrian hamsters

1976

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The distribution of Mg++-activated ATPase was determined with light and electron microscopy in normal and degenerating seminferous tubules. In the normal animals ATPase was localized in the interface between spermatids and Sertoli cells, in association with the cytoplasmic filaments contained within Sertoli cell processes, and in the lymphatic endothelium. ATPase activity increased in degenerating tubules as observed by light microscopy. Electron microscopic investigations of the degenerating tubules which contained only spermatogonia and Sertoli cells revealed reaction product on the outer surface of the Sertoli cell processes and within the interface between adjacent Sertoli cells. Reactaction product was also observed in the Sertoli cell processes between the cytoplasmic filaments and the cell membrane. Where filaments were absent in Sertoli cell processes, no reaction product was observed. These electron microscopic studies indicate that the increase in ATPase activity in testicular degeneration is probably a relative increase due to a loss of the germinal elements of the tubular epithelium and subsequent apposition of the Sertoli cell processes. We speculate that the ATPase activity localized within the Sertoli cell processes may be involved in providing an energy source for filament motility.

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INCREASE OF (Ca2+-Mg2+)-ADENOSINE TRIPHOSPHATASE ACTIVITY IN RAT TESTIS UNDERGOING REGRESSION OF THE GERMINAL EPITHELIUM

Abstract: The pattern of testicular energy metabolism changes when spermatogenesis is impaired. Testes

Cited by 7 publications

References 11 publications

Testosterone Propionate Induced Changes in Testicular Phosphatases of Musk Shrew (Suncus murinus L.)/Testosteronpropionatinduzierte Veränderungen der Hoden-Phosphatasen von Bisammaus (Suncus murinus L.)

Testosterone Propionate Induced Changes in Testicular Phosphatases of Musk Shrew (Suncus murinus L.)/Testosteronpropionatinduzierte Veränderungen der Hoden-Phosphatasen von Bisammaus (Suncus murinus L.)

Histophysiological Changes of the Testicular Tissue Due to Busulphan Administration in the Wild Indian House Rat (Rattus Rattus)

Light and electron microscopic localization of ATPase in normal and degenerating testes of syrian hamsters

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