Increase of CD8+ T-effector memory cells in peripheral blood of patients with relapsing–remitting multiple sclerosis compared to healthy controls

Haegele, K F; Stueckle, Christoph Alexander; Malin, J.‐P.; Sindern, Eckhart

doi:10.1016/j.jneuroim.2006.09.008

Cited by 38 publications

(26 citation statements)

References 17 publications

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“…54 These pathologies are also associated with an accumulation of neutrophils at the inflammatory site 55 or with an increase of IL-8 production, 56 respectively. Moreover, in WG, circulating neutrophils also express MHC II molecules.…”

Section: Discussionmentioning

confidence: 99%

Neutrophils efficiently cross-prime naive T cells in vivo

Beauvillain

Delneste

Scotet

et al. 2007

Blood

266

226

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Neutrophils are professional phagocytes that migrate early, in high number, to the infection sites. Our study has analyzed how neutrophils cross-present antigens and influence CD8 ؉ T-cell responses. By using highly purified neutrophils from peritoneal exudates and bone marrow, we have shown that neutrophils crosspresent ovalbumin to a CD8 ؉ T-cell hybridoma and to naive CD8 ؉ T cells from OT1 transgenic mice. Cross-presentation by neutrophils was TAP and proteasome dependent and was as efficient as in macrophages. Moreover, it actually occurred earlier than in professional antigenpresenting cells. Peritoneal exudate neutrophils from mice injected intraperitoneally with ovalbumin also cross-presented ovalbumin, proving that neutrophils take up and present exogenous antigens into major histocompatibility complex I (MHC I) molecules in vivo. We then evaluated the in vivo influence of antigen crosspresentation by neutrophils on CD8 ؉ Tcell response using ␤2-microglobulindeficient mice transferred with OT1 CD8 ؉ T cells and injected with ovalbuminpulsed neutrophils. Four days after neutrophil injection, OT1 cells proliferated and expressed effector functions (IFN-␥ production and cytolysis). They also responded efficiently to a rechallenge with ovalbumin-pulsed dendritic cells in CFA. These data are the first demonstration that neutrophils cross-prime CD8 ؉ T cells in vivo and suggest that they may constitute, together with professional antigenpresenting cells, an attractive target to induce cytotoxic T cells in vaccines. IntroductionAlthough it was originally thought that peptides presented into major histocompatibility complex I (MHC I) molecules to CD8 ϩ T cells derived exclusively from endogenous proteins, it is now admitted that, under certain circumstances, professional antigenpresenting cells (APCs; dendritic cells [DCs] and macrophages) can present some exogenous antigens in the MHC I molecules. 1,2 This process, called antigen cross-presentation, can occur by 2 different mechanisms. One is dependent on the transporter associated with antigen processing (TAP) that includes proteasomedependent and -independent pathways, and the other one is TAP independent. 3 Due to their ability (1) to migrate from peripheral tissues to the lymph nodes, where they encounter recirculating naive T cells, and (2) to express high levels of costimulatory molecules, DCs are usually considered as the only APC able to prime naive T cells. 4,5 This property has recently been extended to murine macrophages. 6 Activated DCs can present some exogenous proteins to naive CD8 ϩ T cells and induce their differentiation into effector cytotoxic T cells (CTLs). This process, referred to as cross-priming, allows the in vivo generation of protective cytotoxic responses against microorganisms that do not infect DCs or that infect DCs but inhibit their properties. 7 Cross-priming by DCs has opened new perspectives in vaccines based on CTL induction. 7,8 In the absence of licensing, DCs stimulate an abortive response that leads to cross-tolerance...

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Section: Discussionmentioning

confidence: 99%

Neutrophils efficiently cross-prime naive T cells in vivo

Beauvillain

Delneste

Scotet

et al. 2007

Blood

266

226

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“…The CSF cell populations in MS patients have been shown to consist of approximately 60% CD4 þ T lymphocytes (58) with a higher frequency of the regulatory phenotype (59,60) and a higher CD4/CD8 ratio (61), while the frequency of NKT lymphocytes is lower (62) than in NIND controls. Compared to blood, CSF of MS patients showed a relative depletion of CD8 þ effector memory T lymphocytes (63). In relation to disease activity, patients with active MS had higher percentages of activated CD4…”

Section: Inflammatory Neurological Diseasesmentioning

confidence: 97%

Flow cytometric characterization of cerebrospinal fluid cells

Graaf

Jongste

Kraan

et al. 2011

Cytometry Part B Clinical

120

115

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Flow cytometry facilitates the detection of a large spectrum of cellular characteristics on a per cell basis, determination of absolute cell numbers and detection of rare events with high sensitivity and specificity. White blood cell (WBC) counts in cerebrospinal fluid (CSF) are important for the diagnosis of many neurological disorders. WBC counting and differential can be performed by microscopy, hematology analyzers, or flow cytometry. Flow cytometry of CSF is increasingly being considered as the method of choice in patients suspected of leptomeningeal localization of hematological malignancies. Additionally, in several neuroinflammatory diseases such as multiple sclerosis and paraneoplastic neurological syndromes, flow cytometry is commonly performed to obtain insight into the immunopathogenesis of these diseases. Technically, the low cellularity of CSF samples, combined with the rapidly declining WBC viability, makes CSF flow cytometry challenging. Comparison of flow cytometry with microscopic and molecular techniques shows that each technique has its own advantages and is ideally combined. We expect that increasing the number of flow cytometric parameters that can be simultaneously studied within one sample, will further refine the information on CSF cell subsets in low-cellular CSF samples and enable to define cell populations more accurately. V C 2011 International Clinical Cytometry Society

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“…Lymphocyte recirculation isn't randomly but is determined by the expression of homing receptors [43][44][45]. Lymphocytes at different states of differentiation or isolated from distinct tissues reveal significant heterogeneity in homing molecule expression whereby the migratory patterns of these cells may be different [4,16].…”

Section: Within Cd8+ T Cells Similarly To Memory Cd4+ T Cells May Be mentioning

confidence: 99%

Effector and memory CD4+ and CD8+ T cells in the chronic infection process.

Ojdana

Safiejko²,

Lipska³

et al. 2009

Folia Histochem Cytobiol.

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T cell memory in comparison with B cell memory is not well understood. This review focuses on CD8+ and CD4+ memory T cells. In this article we try to define memory cells and also present models of memory T cells formation. We would also like to delineate their differentiation into distinct subsets. Long-lived memory T cells consist in two main subsets: T CM and T EM . Recent studies have shown that not all cells considered to be memory cells differentiate into T CM and T EM , but a small proportion of theses cells exhibit naive cells phenotype. Memory T cells constitute a heterogeneous population of cells. In this study we lay stress on characteristic of main memory T cells subsets and their alleged participation in immune response upon reexposure to the Ag.

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Increase of CD8+ T-effector memory cells in peripheral blood of patients with relapsing–remitting multiple sclerosis compared to healthy controls

Cited by 38 publications

References 17 publications

Neutrophils efficiently cross-prime naive T cells in vivo

Neutrophils efficiently cross-prime naive T cells in vivo

Flow cytometric characterization of cerebrospinal fluid cells

Effector and memory CD4+ and CD8+ T cells in the chronic infection process.

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