Increase of multidrug efflux pump expression in fluoroquinolone-resistant Salmonella mutants induced by ciprofloxacin selective pressure

Kang, Hyun Wol; Woo, Gun Jo

doi:10.1016/j.rvsc.2014.07.019

Cited by 9 publications

(4 citation statements)

References 42 publications

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“…Additionally, the alternative efflux pump candidates emrB and acrF, reported to be overexpressed in quinolone-resistant Salmonella Typhimurium isolates, 40 were also studied and revealed down-regulation only in this mutant. A similar observation has also been reported by Kang and Woo, 55 where expression levels of efflux pump-related genes were tested in ciprofloxacin-resistant S. enterica serovar Istanbul mutants. In all of the mutants, not only soxS, but also acrF, were down-regulated compared with the parental strain whereas acrB, ramA and/or marA were overexpressed.…”

Section: Q17supporting

confidence: 84%

Attenuation ofin vitrohost–pathogen interactions in quinolone-resistantSalmonellaTyphi mutants

Ballesté-Delpierre

Fàbrega

Ferrer-Navarro

et al. 2015

J. Antimicrob. Chemother.

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In this study, we provide an in-depth characterization of the molecular mechanisms of antibiotic resistance in the Salmonella Typhi serovar and evidence that acquisition of antimicrobial resistance is concomitantly detected with a loss of virulence (epithelial cell invasion, macrophage phagocytosis and cytokine production). We suggest that the low prevalence of clinical isolates of Salmonella Typhi highly resistant to ciprofloxacin is due to poor immunogenicity and impaired dissemination ability of these isolates.

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Section: Q17supporting

confidence: 84%

Attenuation ofin vitrohost–pathogen interactions in quinolone-resistantSalmonellaTyphi mutants

Ballesté-Delpierre

Fàbrega

Ferrer-Navarro

et al. 2015

J. Antimicrob. Chemother.

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“…Our results revealed that AcrEF and MdtK efflux may have little contribution to ENR resistance at early stage, while AcrAB, EmrAB and MdfABC may play an important role in ENR resistance, since the expression level of acrB, emrB, mdfA was increased with increased level of FQs resistance and acrB gene was significantly increased, while the expression of the acrF and mdtK gene down-regulated as the susceptibility reduced (Figure 2 and Table 4). Different performance of efflux pumps towards FQ pressure was also reported in the previous study that the expression level of acrB was increased and acrF decreased in CIP-resistant Salmonella with the MIC value ≥2 µg/mL [28].…”

Section: Discussionsupporting

confidence: 61%

“…In addition, mechanisms affecting the cell envelope by increased/decreased expression of OMPs and/or efflux of FQs also contributed to the intracellular accumulation of FQs [21,28]. In our study, the relative expression of outer membrane-related genes (ompC, ompD and ompF) were all down-regulated in the mutants with resistance level less than 8MIC, and the amount of down-regulation decreased with the increase in resistance level.…”

Section: Discussionsupporting

confidence: 51%

The Evolution of Fluoroquinolone Resistance in Salmonella under Exposure to Sub-Inhibitory Concentration of Enrofloxacin

Huang

et al. 2021

IJMS

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The evolution of resistance in Salmonella to fluoroquinolones (FQs) under a broad range of sub-inhibitory concentrations (sub-MICs) has not been systematically studied. This study investigated the mechanism of resistance development in Salmonella enterica serovar Enteritidis (S. Enteritidis) under sub-MICs of 1/128×MIC to 1/2×MIC of enrofloxacin (ENR), a widely used veterinary FQ. It was shown that the resistance rate and resistance level of S. Enteritidis varied with the increase in ENR concentration and duration of selection. qRT-PCR results demonstrated that the expression of outer membrane porin (OMP) genes, ompC, ompD and ompF, were down-regulated first to rapidly adapt and develop the resistance of 4×MIC, and as the resistance level increased (≥8×MIC), the up-regulated expression of efflux pump genes, acrB, emrB amd mdfA, along with mutations in quinolone resistance-determining region (QRDR) gradually played a decisive role. Cytohubba analysis based on transcriptomic profiles demonstrated that purB, purC, purD, purF, purH, purK, purL, purM, purN and purT were the hub genes for the FQs resistance. The ‘de novo’ IMP biosynthetic process, purine ribonucleoside monophosphate biosynthetic process and purine ribonucleotide biosynthetic process were the top three biological processes screened by MCODE. This study first described the dynamics of FQ resistance evolution in Salmonella under a long-term selection of sub-MICs of ENR in vitro. In addition, this work offers greater insight into the transcriptome changes of S. Enteritidis under the selection of ENR and provides a framework for FQs resistance of Salmonella for further studies.

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“…The susceptibility of CI-ST to cefoxitin, ceftriaxone, chloramphenicol, meropenem, ciprofloxacin, and levofloxacin was decreased to 4-, 8-, 4-, 8-, 128-, and 32-fold when compared to WT-ST. CI-ST was resistant to cefoxitin (8 lg/mL), ciprofloxacin (2 lg/mL), and tobramycin (8 lg/mL), and CA-ST was highly resistant to ampicillin ([ 512 lg/mL). The results imply that the ciprofloxacin selective pressure induced MDR CI-ST in association with efflux pumps [16].…”

Section: Resultsmentioning

confidence: 83%

Characterization of β-lactamase- and efflux pump-mediated multiple antibiotic resistance in Salmonella Typhimurium

Uddin

Ahn

2018

Food Sci Biotechnol

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This study aimed to assess the β-lactamase- and efflux pump-mediated antibiotic resistance in Typhimurium (WT-ST), ciprofloxacin-induced antibiotic-resistant. Typhimurium (CI-ST), and clinically-acquired antibiotic-resistant . Typhimurium (CA-ST). The β-lactamase activities were significantly increased up to 63 μmol/min/mL in CA-ST and 24 μmol/min/mL in CI-ST when compared to WT-ST (13 μmol/min/mL). The highest efflux pump activity was observed in CI-ST and CA-ST, showing more than 45%. The antibiotic susceptibilities of WT-ST, CI-ST, and CA-ST were increased in the presence of β-lactamase and efflux pump inhibitors. CA-ST showed the highest activity in AcrD, MdtABC, EmrAB, MdtK, and MacAB efflux pumps. The repressed were responsible for the decreased susceptibility of CA-ST to ampicillin (MIC > 512 μg/mL). This study would provide useful information for better understating of the development of multidrug resistance in association with β-lactamase and efflux pump activities and designing new antibiotic chemotherapy in combination with inhibitors.

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Increase of multidrug efflux pump expression in fluoroquinolone-resistant Salmonella mutants induced by ciprofloxacin selective pressure

Cited by 9 publications

References 42 publications

Attenuation ofin vitrohost–pathogen interactions in quinolone-resistantSalmonellaTyphi mutants

Attenuation ofin vitrohost–pathogen interactions in quinolone-resistantSalmonellaTyphi mutants

The Evolution of Fluoroquinolone Resistance in Salmonella under Exposure to Sub-Inhibitory Concentration of Enrofloxacin

Characterization of β-lactamase- and efflux pump-mediated multiple antibiotic resistance in Salmonella Typhimurium

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